scholarly journals Denervation atrophy is independent from Akt and mTOR activation and is not rescued by myostatin inhibition

2014 ◽  
Vol 7 (4) ◽  
pp. 471-481 ◽  
Author(s):  
E. M. MacDonald ◽  
E. Andres-Mateos ◽  
R. Mejias ◽  
J. L. Simmers ◽  
R. Mi ◽  
...  
Keyword(s):  
Denervation Atrophy ◽  
Myostatin Inhibition

scholarly journals Myostatin Deficiency Protects C2C12 Cells from Oxidative Stress by Inhibiting Intrinsic Activation of Apoptosis

Cells ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1680
Author(s):  
Marius Drysch ◽  
Sonja Verena Schmidt ◽  
Mustafa Becerikli ◽  
Felix Reinkemeier ◽  
Stephanie Dittfeld ◽  
...  
Keyword(s):  
Intracellular Signaling ◽  
Ischemia Reperfusion ◽  
C2c12 Cell ◽  
C2c12 Cells ◽  
Ros Generation ◽  
Protective Effects ◽  
Activity Increase ◽  
Mitogen Activated Protein ◽  
Myostatin Inhibition ◽  
Hypoxia Reoxygenation

Ischemia reperfusion (IR) injury remains an important topic in clinical medicine. While a multitude of prophylactic and therapeutic strategies have been proposed, recent studies have illuminated protective effects of myostatin inhibition. This study aims to elaborate on the intracellular pathways involved in myostatin signaling and to explore key proteins that convey protective effects in IR injury. We used CRISPR/Cas9 gene editing to introduce a Myostatin (Mstn) deletion into a C2C12 cell line. In subsequent experiments, we evaluated overall cell death, activation of apoptotic pathways, ROS generation, lipid peroxidation, intracellular signaling via mitogen-activated protein kinases (MAPKs), cell migration, and cell proliferation under hypoxic conditions followed by reoxygenation to simulate an IR situation in vitro (hypoxia reoxygenation). It was found that mitogen-activated protein kinase kinase 3/6, also known as MAPK/ERK Kinase 3/6 (MEK3/6), and subsequent p38 MAPK activation were blunted in C2C12-Mstn−/− cells in response to hypoxia reoxygenation (HR). Similarly, c-Jun N-terminal kinase (JNK) activation was negated. We also found the intrinsic activation of apoptosis to be more important in comparison with the extrinsic activation. Additionally, intercepting myostatin signaling mitigated apoptosis activation. Ultimately, this research validated protective effects of myostatin inhibition in HR and identified potential mediators worth further investigation. Intercepting myostatin signaling did not inhibit ROS generation overall but mitigated cellular injury. In particular, intrinsic activation of apoptosis origination from mitochondria was alleviated. This was presumably mediated by decreased activation of p38 caused by the diminished kinase activity increase of MEK3/6. Overall, this work provides important insights into HR signaling in C2C12-Mstn−/− cells and could serve as basis for further research.

scholarly journals MYOSTATIN INHIBITION IMPROVES CARDIAC GLUCOSE METABOLISM IN A MURINE MODEL OF OBESITY

2015 ◽  
Vol 65 (10) ◽  
pp. A1009
Author(s):  
Estibaliz Castillero ◽  
Hirokazu Akashi ◽  
Ruiping Ji ◽  
Catherine Wang ◽  
Ziad Ali ◽  
...  
Keyword(s):  
Glucose Metabolism ◽  
Murine Model ◽  
Myostatin Inhibition

Denervation Atrophy of the Tongue after Hypoglossal-Nerve Injury

2012 ◽  
Vol 367 (2) ◽  
pp. 156-156 ◽  
Author(s):  
Luis Junquera ◽  
Lorena Gallego
Keyword(s):  
Nerve Injury ◽  
Hypoglossal Nerve ◽  
Denervation Atrophy

Prevention of Denervation atrophy by Nerve Implantation

2004 ◽  
Vol 20 (07) ◽  
pp. 545-553 ◽  
Author(s):  
Kazuki Ueda ◽  
Kiyonori Harii
Keyword(s):  
Denervation Atrophy ◽  
Nerve Implantation

scholarly journals Myostatin inhibition improves hyperglycemia and hyperphagia in lipodystrophic mice

2012 ◽  
Vol 6 (Suppl 3) ◽  
pp. P7
Author(s):  
Tingqing Guo ◽  
Nichole Bond ◽  
William Jou ◽  
Oksana Gavrilova ◽  
Alexandra C McPherron
Keyword(s):  
Myostatin Inhibition

scholarly journals Combinatory effects of siRNA-induced myostatin inhibition and exercise on skeletal muscle homeostasis and body composition

2014 ◽  
Vol 2 (3) ◽  
pp. e00262 ◽  
Author(s):  
Stephanie Mosler ◽  
Karima Relizani ◽  
Etienne Mouisel ◽  
Helge Amthor ◽  
Patrick Diel
Keyword(s):  
Skeletal Muscle ◽  
Body Composition ◽  
Myostatin Inhibition ◽  
Muscle Homeostasis

Continuous Muscle Fiber Activity Associated with Denervation Atrophy

1982 ◽  
Vol 21 (6) ◽  
pp. 375-379 ◽  
Author(s):  
R. Cotrufo ◽  
G. Di Iorio ◽  
A. Ammendola ◽  
F. Bravaccio
Keyword(s):  
Muscle Fiber ◽  
Denervation Atrophy

Modulation of IGF mRNA abundance during muscle denervation atrophy

1993 ◽  
Vol 25 (9) ◽  
pp. 1005???1008 ◽  
Author(s):  
SUSAN M. CZERWINSKI ◽  
JAN NOVAKOFSKI ◽  
PETER J. BECHTEL
Keyword(s):  
Mrna Abundance ◽  
Denervation Atrophy ◽  
Muscle Denervation
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