The cellular origin of fibronectin in the basement membrane zone of developing tooth

Development ◽  
1986 ◽  
Vol 95 (1) ◽  
pp. 73-80
Author(s):  
Kirsti Hurmerinta ◽  
Pentti Kuusela ◽  
Irma Thesleff
Keyword(s):  
Basement Membrane ◽  
Mesenchymal Cell ◽  
Mesenchymal Cells ◽  
Basement Membrane Zone ◽  
Enamel Organ ◽  
Immunofluorescent Localization ◽  
Cellular Origin ◽  
Chicken Tissue ◽  
Cellular Source ◽  
Species Specific

The cellular source of fibronectin in the dental epitheliomesenchymal interface was studied in interspecies combinations of mouse and quail tissue. Species-specific fibronectin antibodies were produced by immunizing rabbits with purified mouse or chicken fibronectin and by absorbing both antisera with purified heterologous fibronectin and insoluble tissue extract. The absorbed antisera to mouse and chicken fibronectin showed fluorescent staining only in mouse and chicken tissue sections, respectively, but not vice versa. When the mouse mesenchymal dental papilla was combined and cultured either with the mouse enamel organ or with the quail pharyngeal epithelium, mesenchymal cell differentiation was initiated and typical alignment of mesenchymal cells along the basement membrane was seen. Examination with transmission electron microscope revealed a typical bilaminar basal lamina with adherent fibrillar matrix on its mesenchymal aspect. Immunofluorescent localization of fibronectin with the mouse-specific fibronectin antiserum showed a brilliant staining in the mesenchymal tissue and in the basement membrane zone. When the chicken-specific fibronectin antiserum was used, no staining was detected in either tissue recombinations. We have suggested earlier that fibronectin in the dental basement membrane plays an important role during the differentiation of mesenchymal cells into odontoblasts. The present study demonstrates that fibronectin in the basement membrane of the developing tooth is produced exclusively by the differentiating mesenchymal cells.

Epithelial-mesenchymal interactions in the production of basement membrane components in the gut

Development ◽  
1988 ◽  
Vol 102 (2) ◽  
pp. 339-347 ◽  
Author(s):  
P. Simon-Assmann ◽  
F. Bouziges ◽  
C. Arnold ◽  
K. Haffen ◽  
M. Kedinger
Keyword(s):  
Basement Membrane ◽  
Experimental Model ◽  
Type Iv Collagen ◽  
Mesenchymal Cell ◽  
Linear Pattern ◽  
Cellular Origin ◽  
Type Iv ◽  
Species Specific ◽  
Membrane Components

The production and deposition of extracellular matrix proteins and the cellular origin of type-IV collagen have been analysed immunocytochemically in cocultured or transplanted intestinal epithelial-mesenchymal cell associations. In the first experimental model, rat intestinal endodermal cells were cultured on top of confluent monolayers of rat intestinal or skin fibroblastic cells. Under these conditions, interstitial matrix and basement membrane proteins were deposited within the fibroblastic layer over the whole culture period; interactions between the epithelial cells and the fibroblastic cell population, whatever their organ of origin, were required for the production of the basement membrane. In addition, its formation was progressive as assessed by the shift of a spot-like labelling to a continuous linear pattern at the epithelial-mesenchymal interface, and paralleled epithelial cell differentiation. In the second experimental model, chick-rat epithelial-mesenchymal recombinants developed as intracoelomic grafts were used, and the immunocytochemical detection of a basement membrane protein, type-IV collagen, was performed with species-specific antibodies. The major role of the mesenchyme in the deposition of type-IV collagen is supported by the fact that anti-chick but not anti-mammalian antibodies stained this antigen in chick mesenchyme-rat endoderm recombinants. These observations emphasize the role of tissue interactions in the formation of a basement membrane and show that the mesenchymal compartment is the principal endogenous source of type-IV collagen.

Role of laminin polymerization at the epithelial mesenchymal interface in bronchial myogenesis

Development ◽  
1998 ◽  
Vol 125 (14) ◽  
pp. 2621-2629 ◽  
Author(s):  
Y. Yang ◽  
K.C. Palmer ◽  
N. Relan ◽  
C. Diglio ◽  
L. Schuger
Keyword(s):  
Smooth Muscle ◽  
Epithelial Cells ◽  
Basement Membrane ◽  
Mesenchymal Cell ◽  
Cell Types ◽  
Mesenchymal Cells ◽  
Cell Contact ◽  
Alpha Actin ◽  
Muscle Myosin

Undifferentiated mesenchymal cells were isolated from mouse embryonic lungs and plated at subconfluent and confluent densities. During the first 5 hours in culture, all the cells were negative for smooth muscle markers. After 24 hours in culture, the mesenchymal cells that spread synthesized smooth muscle alpha-actin, muscle myosin, desmin and SM22 in levels comparable to those of mature smooth muscle. The cells that did not spread remained negative for smooth muscle markers. SM differentiation was independent of cell-cell contact or proliferation. In additional studies, undifferentiated lung mesenchymal cells were cocultured with lung embryonic epithelial cells at high density. The epithelial cells aggregated into cysts surrounded by mesenchymal cells and a basement membrane was formed between the two cell types. In these cocultures, the mesenchymal cells in contact with the basement membrane spread and differentiated into smooth muscle. The rest of the mesenchymal cells remained round and negative for smooth muscle markers. Inhibition of laminin polymerization by an antibody to the globular regions of laminin beta1/gamma1 chains blocked basement membrane assembly, mesenchymal cell spreading and smooth muscle differentiation. These studies indicated that lung embryonic mesenchymal cells have the potential to differentiate into smooth muscle and the process is triggered by their spreading along the airway basement membrane.

Protein Substrate Alters Cell Physiology in Primary Culture of Vocal Fold Epithelial Cells

2021 ◽  
pp. 1-14
Author(s):  
Emily E. Kimball ◽  
Lea Sayce ◽  
Xiaochuan C. Xu ◽  
Chase M. Kruszka ◽  
Bernard Rousseau
Keyword(s):  
Cell Culture ◽  
Epithelial Cells ◽  
Basement Membrane ◽  
Vocal Fold ◽  
Phase Contrast ◽  
Mesenchymal Cell ◽  
Mesenchymal Cells ◽  
Collagen Iv ◽  
Collagen I ◽  
Protein Substrate

The basement membrane interacts directly with the vocal fold epithelium. Signaling between the basement membrane and the epithelium modulates gene regulation, differentiation, and proliferation. The purpose of this study was to identify an appropriate simple single-protein substrate for growth of rabbit vocal fold epithelial cells. Vocal folds from 3 New Zealand white rabbits (<i>Oryctolagus cuniculus</i>) were treated to isolate epithelial cells, and cells were seeded onto cell culture inserts coated with collagen I, collagen IV, laminin, or fibronectin. Transepithelial electrical resistance (TEER) was measured, and phase contrast microscopy, PanCK, CK14, and E-cadherin immunofluorescence were utilized to assess for epithelial cell-type characteristics. Further investigation via immunofluorescence labeling was conducted to assess proliferation (Ki67) and differentiation (Vimentin). There was a significant main effect of substrate on TEER, with collagen IV eliciting the highest, and laminin the lowest resistance. Assessment of relative TEER across cell lines identified a larger range of TEER in collagen I and laminin. Phase contrast imaging identified altered morphology in the laminin condition, but cell layer depth did not appear to be related to TEER, differentiation, or morphology. Ki67 staining additionally showed no significant difference in proliferation. All conditions had confluent epithelial cells and dispersed mesenchymal cells, with increased mesenchymal cell numbers over time; however, a higher proportion of mesenchymal cells was observed in the laminin condition. The results suggest collagen IV is a preferable basement membrane substrate for in vitro vocal fold epithelial primary cell culture, providing consistent TEER and characteristic cell morphology, and that laminin is an unsuitable substrate for vocal fold epithelial cells and may promote mesenchymal cell proliferation.

scholarly journals IgA Antibodies in Chronic Bullous Disease of Childhood React with a 97kDa Basement Membrane Zone Protein

1996 ◽  
Vol 106 (6) ◽  
pp. 1277-1280 ◽  
Author(s):  
John J. Zone ◽  
Ted B. Taylor ◽  
Donald P. Kadunce ◽  
Tadeusz P. Chorzelski ◽  
Lawrence A. Schachner ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Basement Membrane Zone ◽  
Bullous Disease ◽  
Iga Antibodies
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