The cellular dynamics of pattern formation in the eye of Drosophila

Development ◽  
1985 ◽  
Vol 89 (1) ◽  
pp. 313-331
Author(s):  
Andrew Tomlinson

The establishment and early development of the ommatidial bundles in the presumptive eye of, Drosophila have been investigated using electron microscopic serial sectioning. A progression of developmental stages has been characterized. Initially simple, symmetrically constructed bundles develop into asymmetrical, more complex constructions. Computer graphic reconstruction has been used to show the three-dimensional structure of the various ommatidial bundles. Autoradiography has been used to locate the position of a region of cell division which is intimately associated with the formation of the ommatidial bundles.

Development ◽  
1981 ◽  
Vol 61 (1) ◽  
pp. 87-101
Author(s):  
Robert J. Biggin

Regeneration and grafting experiments were carried out on the prothoracic leg of the cricket Teleogryllus commodus (Walker) to examine the precision with which surface cuticular structures and internal epidermal derivatives are reformed. By comparing regenerated and grafted limbs with normal limbs it was found that the three-dimensional structure of epidermal derivatives is not restored. This is despite the fact that regenerated and grafted limbs appear similar in their external morphology to normal limbs. The implication of these results are discussed in the context of theories of pattern formation.


1997 ◽  
Vol 106 (6) ◽  
pp. 490-494 ◽  
Author(s):  
Kiminori Sato ◽  
Minoru Hirano

An investigation was carried out to determine the fine three-dimensional structure of pericytes in excised human vocal fold mucosa, by means of scanning and transmission electron microscopic observation. The results are summarized as follows. 1) There were many pericytes around the true capillaries, arterial capillaries, and venous capillaries in the vocal fold mucosa. 2) Newborns had pericytes around the capillaries in the vocal fold mucosa. 3) The pericytes had bulged fusiform or polygonal cell bodies and branching processes. The branching processes consisted of long and relatively thick longitudinal ones and short circumferential ones. 4) The cell body and processes of the pericytes encircled the capillaries, and the tips of the processes formed intercellular tight junctions with endothelial cells and made a firm connection with them. 5) The pericytes had many cytoplasmic filaments. 6) The pericytes in the vocal fold mucosa appeared to support and protect capillary walls in the vibrating tissue.


2017 ◽  
Vol 2017.25 (0) ◽  
pp. 412
Author(s):  
Momoko YAMAGISHI ◽  
Takuro HASHIMOTO ◽  
Hitoshi YOKOTA ◽  
Tetuya SUZUKI

Author(s):  
M. Watanabe ◽  
S. Sasaki ◽  
N. Anasawa

Since the electron microscope allows only a two-dimensional image of the specimen to be projected in the direction of the incident beam, it is difficult to represent a three-dimensional arrangement of the specimen substructure from an electron microscopic image. It is also difficult and troublesome to determine the inner specimen structure by means of electron diffraction due to its intrinsic nature. The same is ture of stereo electron microscopic technique. A new method by which one can derive detailed information on the three-dimensional structure of materials is of vital necessity.A method of quantitative determination of three-dimensional structure from electron microscopic images has been reported by De Rosier and Klug. This method makes it possible to derive from electron micrographs, the structures of micro specimens and bio-polymers inadequate for X-ray analysis.


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