Stimulation of division in mouse 3T3 cells by coculture with embryonic chick limb tissue

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 219-226
Author(s):  
K. M. Bell ◽  
J. C. McLachlan
Keyword(s):  
Cell Division ◽  
Normal Development ◽  
Active Regions ◽  
Limb Bud ◽  
Embryonic Chick ◽  
3T3 Cells ◽  
Zone Of Polarizing Activity ◽  
Surgical Manipulation ◽  
Stimulation Of Division ◽  
Stimulation Of

Two regions of the chick limb bud — the apical ectodermal ridge and the zone of polarizing activity — have been shown to influence cell division and pattern formation during normal development and following surgical manipulation. In this study, using a simple coculture system, together with autoradiography, we have shown that these morphogenetically active regions of the limb bud can stimulate quiescent 3T3 cells to initiate DNA synthesis to a significantly greater degree than comparable but morphogenetically inactive regions of the limb bud.

The zone of polarizing activity: evidence for a role in normal chick limb morphogenesis

Development ◽  
1979 ◽  
Vol 50 (1) ◽  
pp. 217-233
Author(s):  
Dennis Summerbell
Keyword(s):  
Normal Development ◽  
Limb Bud ◽  
Concentration Profiles ◽  
Impermeable Barrier ◽  
Limb Morphogenesis ◽  
Zone Of Polarizing Activity

When an impermeable barrier is placed so as to divide the early chick limb-bud into anterior and posterior parts then development continues only on one side of the barrier. The detailed results are inconsistent with mosaic development. They can readily be explained by supposing that pattern is specified by the concentration of a diffusible morphogen controlled by the zone of polarizing activity. A simulation of appropriate concentration profiles is presented and its relevance to similar experiments published elsewhere is discussed. It seems probable that the zone of polarizing activity is active during normal development.

The preliminary characterization of mitogens secreted by embryonic chick wing bud tissues in vitro

Development ◽  
1986 ◽  
Vol 93 (1) ◽  
pp. 257-265
Author(s):  
K. M. Bell
Keyword(s):  
Growth Factors ◽  
Limb Development ◽  
Rat Kidney ◽  
Active Regions ◽  
Limb Bud ◽  
Embryonic Chick ◽  
Bovine Endothelial Cells ◽  
Normal Rat ◽  
Wing Bud

Embryonic chick wing bud tissues secrete diffusible mitogens when cultured in vitro (Bell & McLachlan, 1985). These molecules may play an important role in limb development since media conditioned by morphogenetically active regions of the wing bud possess greater mitogenic activity than media conditioned by non-morphogenetic regions. These studies show that while the chick-derived growth factors were mitogenic for mouse-derived NIH 3T3,10T1/2 and NR6 cells and chick limb bud cells, they did not stimulate DNA synthesis in 3B11, PC13 END, normal rat kidney or bovine endothelial cells. Furthermore, the effects of the chick-derived mitogens were synergistically enhanced by insulin and PGF2α but remained unaffected by ECDGF, EGF, FGF and MSA. These findings indicate that embryonic chick limb bud cells synthesize and secrete growth factors which resemble in function other well-characterized growth factors and in particular PDGF.

The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage

1977 ◽  
Vol 105 (1) ◽  
pp. 99-108 ◽  
Author(s):  
M KLAGSBRUN ◽  
R LANGER ◽  
R LEVENSON ◽  
S SMITH ◽  
C LILLEHEI
Keyword(s):  
Cell Division ◽  
Growth Factor ◽  
Dna Synthesis ◽  
3T3 Cells ◽  
Stimulation Of

Evidence for regulation of growth, size and pattern in the developing chick limb bud

Development ◽  
1981 ◽  
Vol 65 (Supplement) ◽  
pp. 129-150
Author(s):  
Dennis Summerbell
Keyword(s):  
Growth Rate ◽  
Cell Division ◽  
Irradiation Temperature ◽  
Limb Bud ◽  
Regulatory Mechanisms ◽  
Surgical Removal ◽  
Temperature Shock ◽  
X Irradiation ◽  
Zone Of Polarizing Activity ◽  
Regulation Of Growth

This paper examines the hypothesis that the developing chick limb bud has mechanisms for regulating the control of growth, size and pattern. The tests included: surgical removal of selected parts of the limb field, X-irradiation, temperature shock and the manipulation of known limb organizer regions (removal of the apical ectodermal ridge, or the addition of an extra zone of polarizing activity). The results strongly support the idea that there are regulatory mechanisms controlling both the pattern and the size of the limb and suggest that they involve regulation of the growth rate via control of cell division throughout the embryonic period. Possible mechanisms are discussed.

Activation of cell membrane enzymes in the stimulation of cell division

1972 ◽  
Vol 46 (2) ◽  
pp. 605-609 ◽  
Author(s):  
Andrew Sivak ◽  
Brooke T. Mossman ◽  
B.L. Van Duuren
Keyword(s):  
Cell Division ◽  
Cell Membrane ◽  
Membrane Enzymes ◽  
Stimulation Of
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