Trophectodermal carcinoma: mouse teratocarcinomaderived tumour stem cells differentiating into trophoblastic and yolk sac elements

Development ◽  
1985 ◽  
Vol 86 (1) ◽  
pp. 125-141
Author(s):  
I. Damjanov ◽  
A. Damjanov ◽  
P. W. Andrews
Keyword(s):  
Stem Cells ◽  
Giant Cells ◽  
Surface Reactivity ◽  
Yolk Sac ◽  
Electron Microscopic ◽  
Common Precursor ◽  
Visceral Yolk Sac ◽  
Total Cell Population ◽  
Immunohistochemical Techniques ◽  
Cytoplasmic Glycogen

The retransplantable tumour line derived from a spontaneous ovarian murine teratocarcinoma (Fekete & Ferigno, 1952) was cloned and characterized using light and electron microscopic and immunohistochemical techniques. Grown in ascites, the tumour consisted predominantly of stem cells and a small number of differentiated derivatives. The stem cells expressed surface reactivity with antibody to SSEA-3 and Forssman antigen, alkaline phosphatase, focal cytoplasmic reactivity with antibody to SSEA-1, and varying amounts of cytoplasmic glycogen and 3 betahydroxysteroid dehydrogenase. Their cytoskeleton reacted with antibodies to keratin and vimentin. The differentiated derivatives formed approximately 5–15% of the total cell population in ascites and appeared either as giant cells or were characterized by their reactivity with antibodies to H-2 or α-foetoprotein or intracellular and pericellular laminin or high levels of 3 betahydroxysteroid dehydrogenase activity. Solid tumours produced from subcutaneously injected cells had a variegated appearance suggesting, that like the limited differentiation in the ascites, the stem cells can give rise to trophoblastic, as well as parietal and visceral yolk sac elements. On the basis of the presented data the tumour stem cells were considered as representing malignant equivalents of the common precursor of trophoblastic, visceral and parietal yolk sac cells most likely corresponding to trophectoderm. Accordingly, the tumour was designated as trophectodermal carcinoma.

Spatiotemporal expression of three gap junction gene products involved in fetomaternal communication during rat pregnancy

Development ◽  
1991 ◽  
Vol 113 (1) ◽  
pp. 165-181 ◽  
Author(s):  
B. Risek ◽  
N.B. Gilula
Keyword(s):  
Gap Junction ◽  
Post Partum ◽  
Giant Cells ◽  
Yolk Sac ◽  
Chorionic Villi ◽  
Pregnant Uterus ◽  
Decidual Cells ◽  
Visceral Yolk Sac ◽  
Beta 2 ◽  
Specific Regulation

The expression of three different members of the gap junction multigene family, alpha 1 (Cx43), beta 1 (Cx32), and beta 2 (Cx26), was analysed in the rat implantation chamber (a structural unit containing fetal, extraembryonic and maternal components within the pregnant uterus) during mid- and late stages of gestation as well as in the delivering, post-partum and non-pregnant uterus. A differential, spatiotemporal and cell-type-specific regulation of gap junctional coexpression was observed for beta 1 and beta 2 in all epithelia examined (visceral, luminal and glandular), as well as for alpha 1 and beta 2 in decidual cells and keratinocytes of the fetal epidermis. alpha 1 antigen was detected in the mesometrial stroma, mesometrial myometrium, connective tissue, mesothelia of the amnion and visceral yolk sac and in the allantoic mesodermal layer throughout gestation. In addition, expression of alpha 1 in the placental basal zone and trophoblast giant cells coincided with the differentiation of these cells. beta 2 expression was observed prominently in the chorionic villi of the placental labyrinth. The presence of beta 1 and beta 2 in the visceral epithelium (visceral yolk sac = the primary route for embryonic nourishment prior to the formation of the chorioallantoic placenta) and beta 2 in the chorionic villi (placental barrier = the major fetomaternal exchange route) suggests that gap junctions have an important role in fetomaternal communication.

scholarly journals In vivo induction of granulopoiesis in visceral yolksac cells by foetal hepatic factors

Development ◽  
1983 ◽  
Vol 73 (1) ◽  
pp. 87-95
Author(s):  
M. A. Anckaert ◽  
M. Symann
Keyword(s):  
Stem Cells ◽  
Double Diffusion ◽  
Diffusion Chamber ◽  
Yolk Sac ◽  
Hepatic Tissue ◽  
System A ◽  
Visceral Yolk Sac ◽  
Diffusion Chamber Culture ◽  
Adult Bone

In order to evaluate the hypothetical activity of foetal hepatic factors on putative yolk-sac haemopoietic stem cells we used the Double Diffusion Chamber (DDC) technique. The DDC were made of a regulator compartment, where foetal hepatic tissue was introduced and a test compartment where visceral yolk-sac cells were cultured. In this system a hepatic signal induced the yolk-sac stem cells to differentiate along the granulocytic pathway but did not stimulate yolk-sac CFUs growth. Contrary to CFUs originating from foetal liver or adult bone marrow, yolk-sac CFUs do not increase numerically in diffusion chamber culture.

Cellular events during early formation of yolk-sac-derived teratomas

Development ◽  
1982 ◽  
Vol 70 (1) ◽  
pp. 225-240
Author(s):  
H. Sobis ◽  
L. Van Hove ◽  
M. Vandeputte
Keyword(s):  
Stem Cells ◽  
Granulation Tissue ◽  
Morphological Study ◽  
Yolk Sac ◽  
Blastema Formation ◽  
Differentiated Cells ◽  
Cellular Events ◽  
Visceral Yolk Sac ◽  
Poorly Differentiated ◽  
Similarities And Differences

A sequential morphological study of the initial cellular events in teratoma induction by displaced visceral yolk sac after foetectomy in rats was undertaken. This study led to the observation that apart from proliferation of cells displaying definite endodermal or mesodermal characteristics,a population of poorly differentiated cells appeared some days after the surgical procedure. It is very likely that these poorly differentiated cells are stem cells from which differentiated structures originate afterwards by a process of redifferentiation. The development of granulation tissue rich in capillaries seems to enhance this process. Similarities and differences with blastema formation are discussed.

scholarly journals Multiangle light-scattering analysis of murine teratocarcinoma cells.

1979 ◽  
Vol 27 (1) ◽  
pp. 366-370 ◽  
Author(s):  
D E Swartzendruber ◽  
B J Price ◽  
L B Rall
Keyword(s):  
Stem Cells ◽  
Light Scattering ◽  
Flow System ◽  
Yolk Sac ◽  
Squamous Cells ◽  
Teratocarcinoma Cells ◽  
Visceral Yolk Sac ◽  
Yolk Sac Cells

Stem cells of the mouse testicular teratocarcinoma are capable of giving rise in vivo and in vitro to a wide variety of cell and tissue types representative of each embryonic germ layer. Multiangle light-scattering measurements in a flow system have been made on these stem cells and on a variety of their differentiated derivatives. This technique is capable of distinguishing the stem cells from parietal yolk sac cells, visceral yolk sac cells, neuronal cells and squamous cells. However, multipotential stem cells cannot be distinguished from stem cells that are restricted in their development to a single pathway.

scholarly journals Chimera-competent eXtra-Embryonic eNdoderm (XEN) cells established from pig embryos

2020 ◽  
Author(s):  
Chi Park ◽  
Young Jeoung ◽  
Jun Uh ◽  
Kieun Park ◽  
Jessica Bridge ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blastocyst Stage ◽  
Yolk Sac ◽  
Embryonic Cells ◽  
Wide Spread ◽  
Primitive Endoderm ◽  
Visceral Yolk Sac ◽  
First Time

AbstractIn this article, we report for the first time the derivation and characterization of extra-embryonic endoderm (XEN) cells from primitive endoderm (PrE) of porcine (p) embryos. The pXEN cells can be reliably and reproducibly generated from parthenote, in vitro and in vivo derived embryos. The pXEN cells retained all the hallmarks of PrE including expression of canonical PrE and XEN cell markers (GATA4, GATA6, SOX17, SALL4, FOXA2, and HNF4A). Transcriptome analysis further confirmed their XEN cell origin. The pXEN cells when introduced into blastocyst stage embryo contributed to wide-spread chimerism including visceral yolk sac, chorion, as well as embryonic gut and liver primordium in the fetus. The pXEN cells were shown to be an efficient nuclear donor for generating cloned offspring. Taken together, pXEN cells fulfil a longstanding need for a stable, chimera-competent, and nuclear transfer-compatible porcine embryonic cells with applications for agriculture and medicine.Significance StatementWe report for the first time, the derivation and characterization of extraembryonic endoderm (XEN) stem cells from porcine (p) embryos. The pXEN cells can be reliably and reproducibly derived from primitive endoderm precursors. When injected into blastocyst-stage embryos, the pXEN cells have contributed to wide-spread chimerism including visceral yolk sac, chorion of the extraembryonic membranes, as well as definitive endoderm of the fetus, primarily the embryonic gut and liver primordium. Additionally, these XEN cells have proven to be an efficient nuclear donor for generating cloned offspring. These newly discovered stem cells provide a novel model for studying lineage segregation, as well as a source for interspecies chimeras for generating endodermal organs, and for genome editing in livestock.

Sign in / Sign up

Export Citation Format

Share Document