Growth and Ascorbic Acid Content of the Chick Embryo
Ascorbic acid (ASA) is actively synthesized by germinating plant seeds (see Mapson, 1953), and by the embryos of various animal species (refs. in Needham, 1942). Hauge & Garrick (quoted by Needham, 1931) found no ASA in the unincubated hen's egg. This was confirmed by Ray (1934), who showed that the vitamin C content of the chick embryo increases gradually after incubation of the egg. Since the egg is a closed system, it follows that the chick embryo can synthesize its own ASA and that the ASA content of the embryo at any given stage must be the balance between synthesis and utilization. It was, therefore, considered of interest to make daily weighings and ASA estimations throughout development with the more sensitive methods now available in order to examine the possible relations between embryonic weight and ASA content on the one hand, and between growth rate and ascorbic acid concentration on the other.