scholarly journals The transition from oocyte-coded to embryo-coded glucose phosphate isomerase in the early mouse embryo

Development ◽  
1983 ◽  
Vol 78 (1) ◽  
pp. 127-140
Author(s):  
John D. West ◽  
J. F. Green
Keyword(s):  
Cellulose Acetate ◽  
Mouse Embryo ◽  
Mouse Embryos ◽  
Glucose Phosphate Isomerase ◽  
Cellulose Acetate Electrophoresis ◽  
Early Mouse Embryo ◽  
Glucose Phosphate ◽  
Early Mouse

The proportions of glucose phosphate isomerase (GPI-1) allozymes produced by early (Gpi-1sa/Gpi-1sb ♀ × Gpi-1sc/Gpi-1sc ♂)F1 mouse embryos were analysed by quantitative cellulose acetate electrophoresis. Technical controls showed that this system is extremely sensitive, quantitatively reproducible and quite accurate. Genetic controls established that the Gpi-1sa/Gpi-1sb mothers were homozygous for the Gpi-1tb temporal allele, that produces relatively high GPI-1 activity in the oocyte. The oocyte-coded enzyme lasted until about 5½ days post coitum (p.c.) or shortly thereafter. The maternally derived, embryonic Gpi-1s allele was expressed no earlier than the paternally derived allele. This was first expressed between 2½ and 3½ days p.c. In this cross, most of the transition from oocyte-coded to embryo-coded GPI-1 occurred between 2½ and 3½ days p.c.

scholarly journals A new allele of the Gpi-1t temporal gene that regulates the expression of glucose phosphate isomerase in mouse oocytes

1984 ◽  
Vol 44 (2) ◽  
pp. 169-181 ◽  
Author(s):  
John D. West ◽  
Graham Fisher
Keyword(s):  
Cellulose Acetate ◽  
Structural Gene ◽  
The Other ◽  
Glucose Phosphate Isomerase ◽  
Cellulose Acetate Electrophoresis ◽  
Mouse Oocytes ◽  
Glucose Phosphate ◽  
Somatic Tissues ◽  
Developmental Programme

The dimeric enzyme glucose phosphate isomerase (GPI-1) is regulated in oocytes by a cis-acting temporal gene (Gpi-1t) that maps close to the structural gene (Gpi-1s). Quantitative cellulose acetate electrophoresis of GPI-1 allozymes from unfertilized eggs produced by various Gpi-1sa / Gpi-1sb heterozygous females revealed a new Gpi-1t allele that we have designated Gpi-1tc. This allele is present in 101/H mice and a partially congenie stock that carries the Gpi-lsa gene derived from the AKR strain. We have confirmed that Gpi-1tc is closely linked to Gpi-1s and that it is cis-acting. It produces higher levels of GPI-1 in unfertilized eggs than the other two Gpi-lt alleles that are known (Gpi-1ta and Gpi-1tb) but has no effect on GPI-1 in somatic tissues or spermatozoa. This new Gpi-1t allele represents a third developmental programme for GPI-1 expression in oocytes.

A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo

Development ◽  
2002 ◽  
Vol 129 (20) ◽  
pp. 4807-4819 ◽  
Author(s):  
Makoto Ishibashi ◽  
Andrew P. McMahon
Keyword(s):  
Spinal Cord ◽  
Sonic Hedgehog ◽  
Mouse Embryo ◽  
Mouse Embryos ◽  
Cell Populations ◽  
Subsequent Growth ◽  
Early Mouse Embryo ◽  
Present Evidence ◽  
Spinal Cord Dorsal ◽  
Early Mouse

Sonic hedgehog (Shh) is a key signal in the specification of ventral cell identities along the length of the developing vertebrate neural tube. In the presumptive hindbrain and spinal cord, dorsal development is largely Shh independent. By contrast, we show that Shh is required for cyclin D1 expression and the subsequent growth of both ventral and dorsal regions of the diencephalon and midbrain in early somite-stage mouse embryos. We propose that a Shh-dependent signaling relay regulates proliferation and survival of dorsal cell populations in the diencephalon and midbrain. We present evidence that Fgf15 shows Shh-dependent expression in the diencephalon and may participate in this interaction, at least in part, by regulating the ability of dorsal neural precursors to respond to dorsally secreted Wnt mitogens.

scholarly journals Nuclear and cytoplasmic localization of newly synthesized proteins in the early mouse embryo

Development ◽  
1988 ◽  
Vol 103 (1) ◽  
pp. 129-134
Author(s):  
S.K. Howlett ◽  
S.C. Barton ◽  
M.L. Norris ◽  
M.A.H. Surani
Keyword(s):  
Mouse Embryo ◽  
Mouse Embryos ◽  
Cytoplasmic Localization ◽  
Early Mouse Embryo ◽  
Male And Female ◽  
Isolated Nuclei ◽  
Embryonic Genome ◽  
Early Mouse

1-, 2- and 4-cell mouse embryos were labelled with [35S]methionine and the newly synthesized proteins from isolated nuclei were compared with those from cytoplasts and total embryos. There were distinct subsets of translation products present within nuclei compared to those that remained in the cytoplasm. There was no detectable evidence for differences in the presence of newly synthesized proteins in the male and female pronuclei. However, different new proteins associated with nuclei over the time that the embryonic genome becomes active.

Cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 600-601
Author(s):  
A.E. Sutherland ◽  
P.G. Calarco ◽  
C.H. Damsky
Keyword(s):  
Mouse Embryo ◽  
Giant Cells ◽  
Blastocyst Stage ◽  
Integrin Subunit ◽  
Β1 Integrin ◽  
Cell Stage ◽  
Early Mouse Embryo ◽  
Migratory Activity ◽  
Early Mouse ◽  
Cell Matrix Interactions

Cell-extracellular matrix (ECM) interactions mediated by the integrin family of receptors are critical for morphogenesis and may also play a regulatory role in differentiation during early development. We have examined the onset of expression of individual integrin subunit proteins in the early mouse embryo, and their roles in early morphogenetic events. As detected by immunoprecipitation, the α6, αV, β1, and β3 subunits are detected as early as the 4-cell stage, α5 at the hatched blastocyst stage and αl and α3 following blastocyst attachment. We tested the role of these integrins in the attachment and migratory activity of two cell populations of the early mouse embryo: the trophoblast giant cells, which invade the uterine stroma and ultimately contribute to the chorio-allantoic placenta, and the parietal endoderm, which migrates over the inner surface of the trophoblast and ultimately forms Reichert's membrane and the parietal yolk sac. Experiments were done in serum-free medium on substrates coated with laminin (Ln) and fibronectin (Fn). Trophoblast outgrowth occurs on Ln and its E8 fragment (long arm), but not on the E1’ fragment (cross region) (Figs. 1, 2 ). This outgrowth is inhibited by anti-E8, anti-Ln, and by the anti-β1 family antiserum anti-ECMR, but not by anti-αV or the function-perturbing GoH3 antibody that recognizes the α6/β1 integrin, a major Ln (E8) receptor. This suggests that trophoblast outgrowth on Ln or E8 is mediated by a different β1 integrin such as α3/β1. Early stages of trophoblast outgrowth (up to 48 hours) on Fn are inhibited by anti-Fn and by function-perturbing anti-αV antibodies, whereas at later times outgrowth becomes insensitive to anti-αV but remains sensitive to the anti-β1 family antiserum anti-ECMr, indicating that trophoblast cells modulate their interaction with Fn during outgrowth. Trophoblast outgrowth on vitronectin (Vn) is sensitive to anti-αV antibodies throughout the 5-day period examined.

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