Formation of viable chimaeras by aggregation between teratocarcinomas and preimplantation mouse embryos

Development ◽  
1982 ◽  
Vol 67 (1) ◽  
pp. 167-179
Author(s):  
C. L. Stewart
Keyword(s):  
Cell Lines ◽  
In Utero ◽  
Mouse Embryos ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Ec Cells ◽  
Extraembryonic Membranes

The formation of viable teratocarcinoma-adult chimaeras, by aggregation rather than by microinjection, is described. Aggregation chimaeras were produced using two pluripotential EC cell lines, PSA-1/NG-2 and PSA-4/TG12. The frequency and distribution of chimaerism were assessed, for the EC cells, in conceptuses recovered from in utero and in adults. In utero 37% of the morphologically normal conceptuses formed from PSA-l/NG-2 aggregations and 73% of the morphologically normal conceptuses produced from PSA-4/TG12 aggregations were found to be chimaeric. However, the frequency of chimaeric adults formed from both cell lines was lower. The reason for this discrepancy appeared to be that in the chimaeric conceptuses, the predominant tissues colonized by the EC cells were the extraembryonic membranes.

Maternal versus paternal expression of a LacZ transgene in preimplantation mouse embryos: effects of genetic background and 2-cell block

Zygote ◽  
2001 ◽  
Vol 9 (3) ◽  
pp. 219-228
Author(s):  
Irina Neganova ◽  
Martin Augustin ◽  
Ursula Eichenlaub-Ritter ◽  
Harald Jockusch
Keyword(s):  
Genetic Background ◽  
Transgene Expression ◽  
In Utero ◽  
Mouse Embryos ◽  
Preimplantation Embryos ◽  
Parental Origin ◽  
Cell Block ◽  
Maternal Origin ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse

The expression of a transgene NI-ROSA LacZ (LacZtg) trapped into the genes for two presumably untranslated, ubiquitously expressed RNAs, was studied in preimplantation mouse embryos with respect to penetrance (fraction of expressing embryos) and to localisation of β-galactosidase activity. With maternal origin in NMRI mice β-galactosidase was first detected within one dot in the cytoplasm of zygotes at 30 h post-hCG. The staining pattern progressed to small clusters and to dense, homogeneous staining of the entire cytoplasm during further development. Within the NMRI background, penetrance in utero was delayed by at least 6 h when the transgene was of paternal as compared with maternal origin. Paternal transgene expression increased marginally during culture to 50 h after explantation of embryos at 30-48 h post-hCG and remained low or decreased in the ‘2-cell block’. Expression of a paternal transgene in preimplantation embryos developing in utero was further delayed in the maternal MF1 as compared with the NMRI background. In contrast to NMRI × NMRI embryos with paternally derived transgene, expression increased with time during the 2-cell block in MF1 × NMRI embryos. Thus, in the earliest phase of mammalian development expression of this LacZtg is influenced by parental origin, maternal genetic background and environment. The spatial distribution of the gene product is developmentally controlled.

Aggregation between teratocarcinoma cells and preimplantation mouse embryos

Development ◽  
1980 ◽  
Vol 58 (1) ◽  
pp. 289-302
Author(s):  
Colin Stewart
Keyword(s):  
Embryonal Carcinoma ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Mouse Embryos ◽  
Embryonic Cells ◽  
Teratocarcinoma Cells ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Ec Cells ◽  
Derivatives Of

The ability of two embryonal carcinoma (EC) cell lines, F9 and PCI3, to aggregate with preimplantation 8-cell mouse embryos is described. Both adhere to the embryonic cells and subsequently compact with the embryos. The aggregates form blastocysts in culture. The blastocysts sometimes contain the EC cells, located almost always in their inner cell mass. Differentiated derivatives of EC cells, namely PYS-1 and PYS-2, as well as STO fibroblasts do not aggregate with embryos.

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Localization of Intracisternal a Particle Antigens in Neoplastic Cells and Preimplantation Mouse Embryos

1980 ◽  
Vol 38 ◽  
pp. 696-697
Author(s):  
Thomas T.F. Huang ◽  
Patricia G. Calarco
Keyword(s):  
Endoplasmic Reticulum ◽  
Normal Development ◽  
Mouse Embryos ◽  
Neoplastic Cells ◽  
Large Numbers ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Intracisternal A Particle ◽  
Normal Feature

The stage specific appearance of a retravirus, termed the Intracisternal A particle (IAP) is a normal feature of early preimplantation development. To date, all feral and laboratory strains of Mus musculus and even Asian species such as Mus cervicolor and Mus pahari express the particles during the 2-8 cell stages. IAP form by budding into the endoplasmic reticulum and appear singly or as groups of donut-shaped particles within the cisternae (fig. 1). IAP are also produced in large numbers in several neoplastic cells such as certain plasmacytomas and rhabdomyosarcomas. The role of IAP, either in normal development or in neoplastic behavior, is unknown.

Effect of donor bovine serum fractionation on preimplantation mouse embryos in culture

1994 ◽  
Vol 41 (1) ◽  
pp. 257 ◽  
Author(s):  
C.N. Millham ◽  
M.B. Tornesi ◽  
A.T. Palasz ◽  
J. Archer
Keyword(s):  
Bovine Serum ◽  
Mouse Embryos ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Serum Fractionation
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