Dermo-epidermal interactions between birds and mammals: differentiation of cutaneous appendages

Development ◽  
1973 ◽  
Vol 30 (3) ◽  
pp. 587-603
Author(s):  
Danielle Dhouailly
Keyword(s):  
Chorioallantoic Membrane ◽  
Mouse Embryos ◽  
Mouse Epidermis ◽  
Upper Lip ◽  
Morphological Organization ◽  
Stage 4 ◽  
Regional Origin ◽  
Skin Explants ◽  
Hair Development ◽  
Hair Morphogenesis

1. The capacity of skin constituents to participate in feather and hair morphogenesis has been analysed in chick and mouse embryos. 2. Reconstituted homo- and heterospecific skin explants, consisting of epidermis and dermis from both species, were cultured for 8 days on the chorioallantoic membrane of the chick. 3. Recombinants of dorsal 11·5- and 12·5-day mouse epidermis and dorsal 7-day or tarsometatarsal 12-day chick dermis gave rise to stage 2 abnormally elongated hair prepapillae. Associations of plantar 14·5-day mouse epidermis with dorsal 7-day chick dermis formed stage 3 hair papillae. 4. The reverse combinations of dorsal 5- and 6-day chick epidermis and dorsal 11·5- to 14·5-day mouse dermis gave rise to arrested feather buds (with 11·5- and 12·5-day dermis) and to short and aberrant feather filaments (with 12·5-, 13·5- and 14·5-day dermis). These short filaments were characterized by the differentiation of easily recognizable but chaotically arranged barb-ridges. The same type of feather differentiation was obtained in recombinants of normally glabrous epidermis from the comb, midventral apterium, or tarsometatarsum from 10-day chick embryos and 13·5- and 14·5-day dorsal mouse dermis. 5. Control homospecific recombinations formed typical well organized feather filaments or stage 4–5 hair cone follicles. Heterospecific associations of feather- or hair-forming epidermis with dermis from glabrous regions did not differentiate any kind of cutaneous appendages. 6. When distribution of feather filaments was compared in recombinants of chick epidermis with either dorsal pelage hair dermis or upper-lip vibrissal dermis, it was found that the feather pattern conformed with the regional origin of the mouse dermis. 7. It was concluded that, during feather and hair development, the dermis transmits two kinds of morphogenetic messages: one that is apparently non-specific and can therefore be understood and expressed by a foreign epidermis from another zoological class, leading to the formation of feather or hair buds, in conformity with the origin of the epidermis; the other message contains specific cues necessary for specific morphological organization of feather and hair.

scholarly journals Targeted deletion of Crif1 in mouse epidermis impairs skin homeostasis and hair morphogenesis

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Jung-Min Shin ◽  
Dae-Kyoung Choi ◽  
Kyung-Cheol Sohn ◽  
Ji-Young Kim ◽  
Myung Im ◽  
...  
Keyword(s):  
Mouse Epidermis ◽  
Targeted Deletion ◽  
Hair Morphogenesis

scholarly journals Differences between tabby and downless mouse epidermis and dermis in culture

1974 ◽  
Vol 23 (2) ◽  
pp. 219-225 ◽  
Author(s):  
J. A. Sofaer
Keyword(s):  
Hair Follicle ◽  
Control Level ◽  
Chorioallantoic Membrane ◽  
Hair Follicles ◽  
Mouse Epidermis ◽  
Mixed Genotype ◽  
Linked Gene ◽  
Chick Chorioallantoic Membrane ◽  
Tail Hair

The semi-dominant X-linked gene tabby (Ta) in the mouse, and one of its recessive autosomal mimics, downless (dl) each produces a mutant syndrome that includes absence of hairs on the tail due to failure of tail hair follicle initiation. However, whereas downless tails failed to produce hair follicles in culture on the chick chorioallantoic membrane, which is in keeping with the adult phenotype of both downless and tabby mice, tabby tails produced follicles at about 40% of the control level. Furthermore, in contrast to previous findings for downless, the culture of mixed genotype epidermis-dermis combinations provided no evidence of a primary epidermal effect in tabby.

scholarly journals Multi-Walled Carbon Nanotubes Can Promote Brassica napus L. and Arabidopsis thaliana L. Root Hair Development through Nitric Oxide and Ethylene Pathways

2020 ◽  
Vol 21 (23) ◽  
pp. 9109
Author(s):  
Gan Zhao ◽  
Yingying Zhao ◽  
Wang Lou ◽  
Dyaaaldin Abdalmegeed ◽  
Rongzhan Guan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Carbon Nanotubes ◽  
Root Hair ◽  
Hair Growth ◽  
Root Hair Development ◽  
Multi Walled Carbon Nanotubes ◽  
Root Hair Growth ◽  
Hair Development ◽  
Walled Carbon Nanotubes ◽  
Hair Morphogenesis

Here, we report that multi-walled carbon nanotubes (MWCNTs) can promote plant root hair growth in the species analyzed in this study; however, low and excessive concentrations of MWCNTs had no significant effect or even an inhibiting influence. Further results show that MWCNTs can enter rapeseed root cells. Meanwhile, nitrate reductase (NR)-dependent nitric oxide (NO) and ethylene syntheses, as well as root hair formation, were significantly stimulated by MWCNTs. Transcription of root hair growth-related genes were also modulated. The above responses were sensitive to the removal of endogenous NO or ethylene with a scavenger of NO or NO/ethylene synthesis inhibitors. Pharmacological and molecular evidence suggested that ethylene might act downstream of NR-dependent NO in MWCNTs-induced root hair morphogenesis. Genetic evidence in Arabidopsis further revealed that MWCNTs-triggered root hair growth was abolished in ethylene-insensitive mutants ein2-5 and ein3-1, and NR mutant nia1/2, but not in noa1 mutant. Further data placed NO synthesis linearly before ethylene production in root hair development triggered by MWCNTs. The above findings thus provide some insights into the molecular mechanism underlying MWCNTs control of root hair morphogenesis.

Cytoplasmic Organization in the Receptor Cells of the Crista Ampullaris

1972 ◽  
Vol 30 ◽  
pp. 60-61 ◽  
Author(s):  
Robert F. Dunn
Keyword(s):  
Fine Particles ◽  
Apical Cell ◽  
Apical Region ◽  
Receptor Cells ◽  
Cuticular Plate ◽  
Morphological Organization ◽  
Crista Ampullaris ◽  
Cytoplasmic Organization ◽  
High Degree ◽  
General Appearance

Receptor cells of the cristae in the vestibular labyrinth of the bullfrog, Rana catesbiana, show a high degree of morphological organization. Four specialized regions may be distinguished: the apical region, the supranuclear region, the paranuclear region, and the basilar region.The apical region includes a single kinocilium, approximately 40 stereocilia, and many small microvilli all projecting from the apical cell surface into the lumen of the ampulla. A cuticular plate, located at the base of the stereocilia, contains filamentous attachments of the stereocilia, and has the general appearance of a homogeneous aggregation of fine particles (Fig. 1). An accumulation of mitochondria is located within the cytoplasm basal to the cuticular plate.

Human Adenovirus Uptake by Preirnplantation Mouse Embryos

1972 ◽  
Vol 30 ◽  
pp. 268-269
Author(s):  
D. G. Chase ◽  
W. Winters ◽  
L. Piko
Keyword(s):  
Hela Cells ◽  
Human Adenovirus ◽  
Mouse Embryos ◽  
Equilibrium Density ◽  
Cell Stage ◽  
Virus Attachment ◽  
Preimplantation Mouse Embryos ◽  
Embryo Culture Medium ◽  
Preimplantation Mouse ◽  
Mouse Cells

Although the outlines of human adenovirus entry and uncoating in HeLa cells has been clarified in recent electron microscope studies, several details remain unclear or controversial. Furthermore, morphological features of early interactions of human adenovirus with non-permissive mouse cells have not been extensively documented. In the course of studies on the effects of human adenoviruses type 5 (AD-5) and type 12 on cultured preimplantation mouse embryos we have examined virus attachment, entry and uncoating. Here we present the ultrastructural findings for AD-5.AD-5 was grown in HeLa cells and purified by successive velocity gradient and equilibrium density gradient centrifugations in CsCl. After dialysis against PBS, virus was sedimented and resuspended in embryo culture medium. Embryos were placed in culture at the 2-cell stage in Brinster's medium.

Alterations in the number of motoneurons containing immunoreactive calcitonin gene-related peptide(CGRP)& choline acetyltransferase(ChAT) in the cervical spinal cord of the wobbler mouse during the development of the motoneuron disease

1995 ◽  
Vol 53 ◽  
pp. 970-971
Author(s):  
L. Vacca-Galloway ◽  
Y.Q. Zhang ◽  
P. Bose ◽  
S.H. Zhang
Keyword(s):  
Spinal Cord ◽  
Early Stage ◽  
Cervical Spinal Cord ◽  
Wild Type Mouse ◽  
Reflex Response ◽  
Mouse Strains ◽  
Behavioral Tests ◽  
Wobbler Mouse ◽  
Stage 4 ◽  
Stage 1

The Wobbler mouse (wr) has been studied as a model for inherited human motoneuron diseases (MNDs). Using behavioral tests for forelimb power, walking, climbing, and the “clasp-like reflex” response, the progress of the MND can be categorized into early (Stage 1, age 21 days) and late (Stage 4, age 3 months) stages. Age-and sex-matched normal phenotype littermates (NFR/wr) were used as controls (Stage 0), as well as mice from two related wild-type mouse strains: NFR/N and a C57BI/6N. Using behavioral tests, we also detected pre-symptomatic Wobblers at postnatal ages 7 and 14 days. The mice were anesthetized and perfusion-fixed for immunocytochemical (ICC) of CGRP and ChAT in the spinal cord (C3 to C5).Using computerized morphomety (Vidas, Zeiss), the numbers of IR-CGRP labelled motoneurons were significantly lower in 14 day old Wobbler specimens compared with the controls (Fig. 1). The same trend was observed at 21 days (Stage 1) and 3 months (Stage 4). The IR-CGRP-containing motoneurons in the Wobbler specimens declined progressively with age.

Preplate neurons in embryonic mouse cortex: Ultrastructural observations using photoconversion of the fluorescent lipophilic dye dil

1992 ◽  
Vol 50 (1) ◽  
pp. 906-907
Author(s):  
Linda C. Hassinger ◽  
James E. Crandall
Keyword(s):  
Mouse Embryos ◽  
Embryonic Mouse ◽  
Cortical Afferents ◽  
Mouse Cortex ◽  
Carbocyanine Dye ◽  
Increased Sensitivity

We have begun to look directly at small numbers of afferent axons to early generated neurons that form the preplate in the developing mouse cortex. The carbocyanine dye Dil (1’1, dioctadecyl-3,3,3’3’-tetramethyl-indocarbocyanine) has proved especially useful for this goal. DiI labels axons and their terminals with greater sensitivity and without some of the disadvantages of axon filling with HRP. The increased sensitivity provided by labeling embryonic axons with DiI has given us new insights into the development of cortical afferents. For instance, we reported originally that afferents from the thalamus were present below the cortex as early as embryonic day 15 (E15) based on HRP injections into mouse embryos. By using DiI placements into the thalamus in aldehyde-fixed brains, we now know that thalamic fibers reach the cortex 24 hrs earlier.

Localization of Intracisternal a Particle Antigens in Neoplastic Cells and Preimplantation Mouse Embryos

1980 ◽  
Vol 38 ◽  
pp. 696-697
Author(s):  
Thomas T.F. Huang ◽  
Patricia G. Calarco
Keyword(s):  
Endoplasmic Reticulum ◽  
Normal Development ◽  
Mouse Embryos ◽  
Neoplastic Cells ◽  
Large Numbers ◽  
Preimplantation Mouse Embryos ◽  
Preimplantation Mouse ◽  
Intracisternal A Particle ◽  
Normal Feature

The stage specific appearance of a retravirus, termed the Intracisternal A particle (IAP) is a normal feature of early preimplantation development. To date, all feral and laboratory strains of Mus musculus and even Asian species such as Mus cervicolor and Mus pahari express the particles during the 2-8 cell stages. IAP form by budding into the endoplasmic reticulum and appear singly or as groups of donut-shaped particles within the cisternae (fig. 1). IAP are also produced in large numbers in several neoplastic cells such as certain plasmacytomas and rhabdomyosarcomas. The role of IAP, either in normal development or in neoplastic behavior, is unknown.

Sign in / Sign up

Export Citation Format

Share Document