Effect of erythropoietin on haemoglobin synthesis and haem synthesizing enzymes of mouse foetal liver cells in culture

Development ◽  
1972 ◽  
Vol 27 (3) ◽  
pp. 525-532
Author(s):  
R. I. Freshney ◽  
John Paul ◽  
David Conkie
Keyword(s):  
Enzyme Activity ◽  
Mouse Liver ◽  
Liver Cells ◽  
Short Term ◽  
Embryonic Mouse ◽  
Cells In Culture ◽  
Foetal Liver ◽  
Rate Limiting ◽  
Haemoglobin Synthesis

The activities of aminolaevulinate synthetase, aminolaevulinate dehydratase and haem synthetase have been examined in short-term cultures of embryonic mouse liver. Although synthesis of haemoglobin was induced by erythropoietin in these cultures no increase in activity was detected in any of the three enzymes over 24 h in culture. In each case, however, enzyme activity was higher when erythropoietin was present than in its absence. The significance of these findings is discussed in relation to control of haemoglobin synthesis and it is concluded that enzyme activity is not rate limiting during induction of haemoglobin synthesis in vitro.

Lowered antioxidant enzymes in spontaneously transformed embryonic mouse liver cells in culture

1993 ◽  
Vol 14 (7) ◽  
pp. 1457-1463 ◽  
Author(s):  
Yi Sun ◽  
Larry W. Oberley ◽  
Terry D. Oberley ◽  
James H. Elwell ◽  
Elaine Sierra-Rivera
Keyword(s):  
Antioxidant Enzymes ◽  
Mouse Liver ◽  
Liver Cells ◽  
Embryonic Mouse ◽  
Cells In Culture

The activities of three enzymes of haem synthesis during hepatic erythropoiesis in the mouse embryo

Development ◽  
1971 ◽  
Vol 26 (2) ◽  
pp. 313-322
Author(s):  
R. I. Freshney ◽  
J. Paul
Keyword(s):  
Enzyme Activity ◽  
Maximal Activity ◽  
Cell Types ◽  
Population Changes ◽  
Adult Liver ◽  
Foetal Liver ◽  
Different Cell Types ◽  
Rate Limiting ◽  
Haemoglobin Synthesis ◽  
Liver Cell Population

Aminolaevulinate synthetase, aminolaevulinate dehydratase, and haem synthetase, three enzymes which may have a regulatory role in haem synthesis, have been determined in liver extracts from different foetal stages of the mouse. Haemoglobin synthesis increases rapidly from early on the 14th day, after fertilization, to reach a maximum late on the 15th day. Aminolaevulinate synthetase reaches a maximum on the 14th day, 24–36 h before the peak of haemoglobin synthesis, aminolaevulinate dehydratase on the 15th day, about 12 h before the peak of haemoglobin synthesis, and haem synthetase on the 17th day. Maximal activity of aminolaevulinate synthetase and aminolaevulinate dehydratase is of only a few hours' duration. Throughout embryonic development the activities of all three enzymes are higher than in the adult liver. The absence of a correlation of enzyme activity with foetal liver cell population changes implies that fluctuations in enzyme activity cannot be explained solely by changes in the proportions of different cell types. The high levels of activity relative to those of adult liver may be related to the high proportion of erythroid cells in the foetal liver. It is concluded that these enzymes are unlikely to form rate-limiting steps during the increase in haemoglobin synthesis between 14 and 15 days.

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (2) ◽  
pp. 299-304 ◽  
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

OBSERVATIONS ON THE EFFECTS OF METHANOL AND FORMALDEHYDE ON ESTABLISHED CELL LINES CULTIVATED IN VITRO

1963 ◽  
Vol 41 (1) ◽  
pp. 299-304
Author(s):  
Alice Elliott ◽  
Donald M. Pace
Keyword(s):  
Cell Lines ◽  
Mouse Liver ◽  
Human Lung ◽  
Liver Cells ◽  
Short Term ◽  
Skin Cells ◽  
Established Cell Lines ◽  
Established Cell ◽  
High Concentrations

Short-term experiments were used to investigate the effects of various concentrations of methanol and formaldehyde upon cells grown in vitro. Mouse liver epithelial, HeLa, human lung, and skin cells were exposed to several concentrations of methanol. The same cell lines, except HeLa, were subjected to different concentrations of formaldehyde.Relatively high concentrations of methanol were required to produce rapid toxic effects. Not all cell lines responded alike to methanol. Concentrations of 15 mg/ml were decidedly inhibitory in case of human skin, lung, and HeLa cells. This concentration, however, appeared to enhance growth in liver cells. At higher concentrations the methanol was toxic to liver cells as well as to the others.Concentrations of formaldehyde greater than 0.035 mg/ml were toxic to mouse liver, human lung and skin cells. A concentration of 0.01 mg/ml of formaldehyde inhibited proliferation of these same cells. These three cell lines did not appear to differ significantly in respect to their sensitivity to formaldehyde. Of the substances investigated to date, formaldehyde appears to be the most toxic.

Effect of acute exposure to PFOA on mouse liver cells in vivo and in vitro

2017 ◽  
Vol 24 (31) ◽  
pp. 24201-24206 ◽  
Author(s):  
Xinmou Wu ◽  
Minqing Liang ◽  
Zhao Yang ◽  
Min Su ◽  
Bin Yang
Keyword(s):  
Mouse Liver ◽  
Liver Cells ◽  
Acute Exposure

scholarly journals CHANGES OF 8-OXO-2'-DEOXYGUANOSINE LEVEL IN MOUSE LIVER CELLS DNA IN CASE OF ACUTE TOXIC STRESS

2016 ◽  
Vol 11 (6) ◽  
pp. 68-74
Author(s):  
N. V. Marmiy ◽  
D. S. Esipov
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Tissue ◽  
Mouse Liver ◽  
Pathological Change ◽  
Liver Cells ◽  
Antioxidant Systems ◽  
Toxic Stress ◽  
Laboratory Mice ◽  
Short Term ◽  
Initial Injection

The changes of the 8-oxo-2'-deoxyguanosine (8-oxo-dG)/dG ratio in the DNA of laboratory mice hepatocytes under the influence of toxic stress were studied. It was shown that the injection of carbon tetrachloride causes the growth of 8-oxo-dG level. A rapid increase in the level of 8-oxo-dG in DNA occurs during the first day of the experiment at short-term toxic stress. Subsequently, 48 hours after the initial injection, the level of 8-oxo-dG decreases to the control values. This change in the value of the biomarker can be attributed to the activation of the reparative and antioxidant systems. The subsequent injection results again in an increase of 8-oxo-dG level, and the latter only increases thereafter. This reflects the exhaustion of the reparative potential of the organism and accompanies the progress of inflammation and pathological change of the liver tissue.

Kinetics of sulphate conjugation in extracts of human foetal liver cells in culture

1988 ◽  
Vol 37 (24) ◽  
pp. 4629-4633 ◽  
Author(s):  
Theresa M.C. Tan ◽  
K.H. Sit ◽  
Kim Ping Wong
Keyword(s):  
Liver Cells ◽  
Cells In Culture ◽  
Foetal Liver ◽  
Kinetics Of ◽  
Human Foetal Liver

Short-Term Retinoic Acid Treatment Increases In Vivo, but Decreases In Vitro, Epidermal Transglutaminase-K Enzyme Activity and Immunoreactivity

1992 ◽  
Vol 99 (3) ◽  
pp. 283-288 ◽  
Author(s):  
Christopher E M Griffiths ◽  
Dean S Rosenthal ◽  
Ambati P Reddy ◽  
James T Elder ◽  
Anders. Astrom ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Retinoic Acid ◽  
Acid Treatment ◽  
Short Term ◽  
Retinoic Acid Treatment ◽  
Epidermal Transglutaminase

Effects of erythropoietin on cell populations and macromolecular syntheses in foetal mouse erythroid cells

Development ◽  
1973 ◽  
Vol 29 (2) ◽  
pp. 453-472
Author(s):  
John Paul ◽  
David Conkie ◽  
Hugo Burgos
Keyword(s):  
Dna Synthesis ◽  
Mouse Liver ◽  
Rna Synthesis ◽  
Normal Development ◽  
Cell Populations ◽  
Erythroid Cells ◽  
Short Term ◽  
Cell Compartments ◽  
Normal Series

The effects of erythropoietin on maturation of erythroid cells were studied in short-term cultures of foetal mouse liver. Erythropoietin-treated cultures had about 50% more cells than untreated cultures after 24 h. The increase occurred in basophilic, polychromatic and orthochromatic erythroblasts as well as in reticulocytes. A striking feature of erythropoietin-treated cultures was the formation of macro-erythroblasts and macrocytes. Autoradiographic studies indicated that a maturation division was omitted in the formation of these cells and that macrocytes might be derived directly from polychromatic erythroblasts or earlier stages. These studies also indicated that there might be no more than three divisions during the normal development of orthochromatic erythroblasts from proerythroblasts in vitro. The mitotic index of proerythroblasts was raised in erythropoietin-treated cultures within 1–2 h. Moreover, erythropoietin caused a marked elevation of DNA synthesis in proerythroblasts within the first hour of culture but effects on DNA synthesis in other cell compartments were not pronounced. In contrast, an increase in RNA synthesis was noted in proerythroblasts and basophilic and polychromatic erythroblasts; it was greatest in the two former compartments. The stimulation was noticeable, especially in the basophilic compartment, within 2 h but increased progressively throughout the first 5–6 h. Erythropoietin did not induce an increase in total protein synthesis in cells of the normal series but macro-erythroblasts exhibited proportionately more grains.

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