Stimulation of cell division in pronephros of embryonic grafts following partial nephrectomy in the host (Xenopus laevis)

Development ◽  
1970 ◽  
Vol 24 (3) ◽  
pp. 525-533
Author(s):  
D. P. Chopra ◽  
J. D. Simnett
Keyword(s):  
Cell Division ◽  
Xenopus Laevis ◽  
Mitotic Activity ◽  
Partial Nephrectomy ◽  
Vascular Supply ◽  
Significant Difference ◽  
And Control ◽  
Mitotic Inhibitor ◽  
Embryonic Grafts ◽  
Stimulation Of

Following partial nephrectomy in juvenile metamorphosed Xenopus laevis the mitotic activity in the regenerating kidney reached its maximum on the 6th day and returned to its normal level by the 16th day. The mitotic activity was measured in the pronephros and epidermis of prefeeding Xenopus larvae (stage 38) at different intervals after their implantation into the lymph sacs of partially nephrectomized and control metamorphosed hosts. Ten days after partial nephrectomy, during the period of increased mitotic activity in the regenerating kidney of the host, the mitotic activity in the implant pronephros was twice as high as that in the implant pronephros of the control hosts. Eighteen days after partial nephrectomy when the mitotic activity in the regenerating host kidney had returned to normal, there was no difference between the mitotic activity of pronephros of implants in nephrectomized and control hosts. There was no significant difference between the mitotic activity of epidermis of the implants in nephrectomized and control hosts, nor was there any difference between the epidermal mitotic activity in implants examined 10 and 18 days after host nephrectomy. It was concluded that a circulating factor (or factors) responsible for the control of mitotic activity in the regenerating host kidney enters the implant through its vascular supply and influences the mitotic activity in the homologous embryonic tissue. It is possible that this factor is a tissue-specific mitotic inhibitor synthesized by the host kidney.

Tissue-specific mitotic inhibition in the kidneys of embryonic grafts and partially nephrectomized host Xenopus laevis

Development ◽  
1971 ◽  
Vol 25 (3) ◽  
pp. 321-329
Author(s):  
D. P. Chopra ◽  
J. D. Simnett
Keyword(s):  
Cell Division ◽  
Xenopus Laevis ◽  
Partial Nephrectomy ◽  
Liver Extract ◽  
Rat Kidney ◽  
Control Level ◽  
Metaphase Arrest ◽  
Tissue Specific ◽  
Mitotic Inhibition ◽  
Embryonic Grafts

Immature Xenopus laevis were unilaterally nephrectomized and implanted subcutaneously with stage 38 (prefeeding) larvae. Rat kidney extract was injected 8 and 16 days after the operation and the mitotic incidence (MI) in the host and implant tissues was measured by the colcemid metaphase arrest technique. The MI in the kidney of nephrectomized hosts was higher than normal at 8 days but had returned to the control level at 16 days. Injection of kidney extract inhibited mitosis in the host kidney both at 8 and 16 days after partial nephrectomy. The MI of implant pronephros was higher in nephrectomized than in control hosts both at 8 and 16 days. Injection of kidney extract into nephrectomized hosts inhibited mitosis in the implant pronephros both at 8 and 16 days. Neither host nephrectomy nor injection of kidney extract had any effect on the MI in the epidermis of the implants. Unoperated immature Xenopus were injected with rat kidney or liver extracts. Kidney extract inhibited mitosis in the kidney but not in the liver, while liver extract inhibited mitosis in the liver but not in the kidney. The rate of mitosis in the kidney and liver of normal animals may be controlled by tissuespecific inhibitors of cell division.

Somatosensory Evoked Potentials in Schizophrenia a Lateralisation Study

1990 ◽  
Vol 157 (6) ◽  
pp. 881-887 ◽  
Author(s):  
Paul Furlong ◽  
Paul Barczak ◽  
Gwilym Hayes ◽  
Graham Harding
Keyword(s):  
Index Finger ◽  
Objective Measure ◽  
Control Group ◽  
Temporal Region ◽  
Control Groups ◽  
Significant Difference ◽  
Schizophrenic Patients ◽  
Left And Right ◽  
And Control ◽  
Stimulation Of

The SSEPs obtained from 19 schizophrenics defined by RDC, DSM–III and PSE criteria Were compared with those from a control group of healthy volunteers. Previous findings of an abnormal lack of lateralising response in schizophrenic patients were not replicated. No significant difference in either amplitude or morphology between the traces obtained from the two groups were recorded. Ipsilateral and contralateral latencies for stimulation of the left and right index finger showed no significant difference in peak latency for any component between patient and control group. When mean peak-to-peak amplitudes were plotted the contralateral component was always greater in amplitude than the ipsilateral one. An objective measure of the degree of lateralisation, the percentage lateralisation quotient, showed no lateralisation differences between the patient and control groups. A case of myogenic contamination of ipsilateral components was observed calling into doubt findings where no temporal region monitoring has been performed.

THE EFFECTS OF GROWTH HORMONE AND CORTICOTROPHIN UPON THE ADRENAL WEIGHT AND ADRENOCORTICAL MITOTIC ACTIVITY IN THE HYPOPHYSECTOMIZED RAT

1955 ◽  
Vol 12 (3) ◽  
pp. 174-184 ◽  
Author(s):  
D. B. CATER ◽  
M. P. STACK-DUNNE
Keyword(s):  
Growth Hormone ◽  
Cell Division ◽  
Mitotic Activity ◽  
Great Increase ◽  
Adrenal Weight ◽  
Synergic Effect ◽  
Single Test ◽  
Pituitary Growth Hormone ◽  
Artificial Mixtures ◽  
Stimulation Of

SUMMARY 1. Treatment of rats 17–24 days after hypophysectomy with pituitary growth hormone for 3 days causes a definite, although limited, increase in adrenal weight. When growth hormone and corticotrophin are given simultaneously, the increase in adrenal weight is greater than the sum of the effects with the hormones separately. The larger the dose of corticotrophin the greater the synergic effect. 2. The action of growth hormone on the adrenal is characterized by a striking stimulation of cell division with relatively little effect on adrenal weight. Corticotrophin also stimulates adrenocortical mitotic activity, but to obtain mitotic activity equivalent to that with growth hormone, doses must be given sufficient to cause a great increase in adrenal weight. This is true whether corticotrophin is injected in saline or in a beeswax/arachis oil mixture. 3. The characteristic activity of each preparation in stimulating the adrenal can be expressed in terms of a quotient, Qmw, the ratio of mitosis-stimulating activity to increase of adrenal weight in a single test. This index is effectively independent of the absolute responses. For growth hormone, Qmw is high; for biologically pure corticotrophin, it is low. Artificial mixtures of the two hormones give intermediate values of the quotient; crude corticotrophin preparations give values higher than those for purified preparations.

Bone marrow cellularity and Na and K levels in fasting and inanition after bile duct ligation

1961 ◽  
Vol 200 (6) ◽  
pp. 1207-1210 ◽  
Author(s):  
James W. Archdeacon ◽  
William R. Markesbery ◽  
Bailey R. Binford
Keyword(s):  
Bile Duct ◽  
Bile Duct Ligation ◽  
Plasma Potassium ◽  
Duct Ligation ◽  
Significant Difference ◽  
Stimulation Of Erythropoiesis ◽  
And Control ◽  
Marrow Cellularity ◽  
Sodium And Potassium ◽  
Stimulation Of

Femur marrow from fasted, bile duct ligated, and control rabbits was examined with supravital stains for occurrence of nucleated cells, including reticulocytes. There were marked decreases in concentration of these cellular elements in both treated groups of rabbits, although the reticulocyte population in the marrow of the bile duct ligated rabbit was considerably higher than in the fasted rabbit. Apparently there was more stimulation of erythropoiesis in the marrow of the jaundiced rabbit in spite of body wt. loss comparable to that of the fasted rabbit. Sodium and potassium analyses of marrow indicated no significant difference in fasted marrow from control marrow, the Na/K ratio being about 0.5. However, both sodium and potassium rose significantly in the bile duct ligated marrow, although the Na/K ratio was not appreciably altered. Also, there was evidence of plasma potassium increase with sodium decrease in the bile duct ligated rabbit.

scholarly journals Visual, instrumental, mycological and mycotoxicological characterization of wheat inoculated with and protected against Alternaria spp.

2016 ◽  
Vol 70 (3) ◽  
pp. 257-264 ◽  
Author(s):  
Elizabet Janic-Hajnal ◽  
Miona Belovic ◽  
Dragana Plavsic ◽  
Jasna Mastilovic ◽  
Ferenc Bagi ◽  
...  
Keyword(s):  
Colour Properties ◽  
Fusarium Spp ◽  
Alternaria Toxins ◽  
Significant Difference ◽  
Colour Parameters ◽  
And Control ◽  
Visual Properties ◽  
Mycotoxigenic Fungus ◽  
Stimulation Of

The aim of this work was to characterize visual properties, instrumentally measured colour properties, field fungi presence and Alternaria toxins levels in wheat samples grown under conditions aimed at inhibition and stimulation of wheat infection with fungi from the Alternaria genus. Experiment was carried out on the wheat treated by fungicide and wheat inoculated by Alternaria spp., while non treated wheat was used as a control. Statistically significant difference was observed between all three treatments using visual scale. Protected wheat samples were significantly different from other samples in terms of all measured colour parameters while inoculated and control wheat samples were significantly different in terms of lightness and dominant wavelength. Identification of field fungi in the all examined wheat samples showed that the dominant mycotoxigenic fungus was Alternaria spp., followed by Fusarium spp. The content of Alternaria toxins in samples of wheat hulls and dehulled kernels point out at higher concentrations of Alternaria toxins in hulls than in dehulled kernels.

CHROMATOGRAPHY OF THE 17-KETOGENIC STEROIDS IN THE DIAGNOSIS AND CONTROL OF CONGENITAL ADRENAL HYPERPLASIA

1960 ◽  
Vol XXXIII (II) ◽  
pp. 230-250 ◽  
Author(s):  
Eileen E. Hill
Keyword(s):  
Congenital Adrenal Hyperplasia ◽  
List Type ◽  
Adrenal Hyperplasia ◽  
Normal Children ◽  
Paediatric Practice ◽  
The Mean ◽  
Cortisone Therapy ◽  
And Control ◽  
Clinical Problems ◽  
Stimulation Of

ABSTRACT A method for the fractionation of the urinary 17-ketogenic steroids with no oxygen grouping at C11 and those oxygenated at C11, is applied to the clinical problems of congenital adrenal hyperplasia. In normal children the mean ratio of the non-oxygenated to oxygenated steroids is 0.24. In childrern with congenital adrenal hyperplasia the ratio is 2.3. The reason for this difference in ratio is discussed. The changes in ratio found under stimulation of the adrenal gland with exogenous or endogenous corticotrophin and the suppression with cortisone therapy are studied. This test can be applied to isolated samples of urine, a major advantage in paediatric practice, and can be carried out in routine laboratories. It is found to be reliable in the diagnosis and sensitive in the control of congenital adrenal hyperplasia.

IOP Lowering Effects of Ocimum basilicum Seed Extract in Two Rabbit Models of Ocular Hypertension

2019 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Renu Agarwal ◽  
SK Gupta ◽  
Sushma Srivastava ◽  
Rohit Saxena
Keyword(s):  
Ocular Hypertension ◽  
Antioxidant Properties ◽  
Ocimum Basilicum ◽  
Seed Extract ◽  
Water Loading ◽  
Antiglaucoma Medication ◽  
Lowering Effect ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Introduction: Ocimum basilicum (OB), a herb known for its antihypertensive, anticholinesterase and antioxidant properties was investigated for possible intraocular pressure (IOP) lowering effects in rabbits with ocular hypertension (OHT). Methods: The IOP lowering effect of a single drop of OB extract (OBE) was evaluated in oculonormotensive rabbits using three concentrations (0.25, 0.5 and 1% w/v). The concentration showing maximum IOP reduction was further evaluated in rabbits with water-loading and steroid-induced OHT. Results: IOP lowering effect of OBE 0.5% in oculonormotensive rabbit eyes was significantly greater compared to OBE 0.25% (p<0.05) but was comparable (p>0.05) to OBE 1%. Therefore, 0.5% concentration was selected for further evaluation. Pretreatment with OBE (0.5%) caused significantly lower increase in IOP after water loading amounting to 23.39% above baseline as compared to 54.00% in control eye, 15 minutes post water loading. At 60 minutes, post water loading, mean IOP rise was 95.12% and 63.58% in control and test eyes, respectively. Significant difference between the mean IOP of two eyes persisted during the 2nd hr. In rabbits with steroid induced OHT, OBE 0.5% produced a mean IOP reduction of 24.73% at the end of first hr and the mean peak IOP reduction of 31.63% was observed at the end of 2 hr. A significant difference between the IOP of test and control eyes persisted from 1 to 6 hr. Conclusions: Ocimum basilicum seed extract showed significant IOP lowering effect in rabbits with water loading and steroid induced OHT, however, its utility as an effective antiglaucoma medication needs further investigations.

Effects of Virgin Coconut Oil as Adjunct Therapy in the Treatment of Allergic Rhinitis

2016 ◽  
Vol 1 (1) ◽  
pp. 22
Author(s):  
Nazli Zainuddin ◽  
Nurul Azira Mohd Shah ◽  
Rosdan Salim
Keyword(s):  
Allergic Rhinitis ◽  
Side Effects ◽  
Coconut Oil ◽  
Test Group ◽  
Nasal Symptom ◽  
Control Group ◽  
Virgin Coconut Oil ◽  
Control Groups ◽  
Significant Difference ◽  
And Control

Introduction: The role of virgin coconut oil in the treatment of allergic rhinitis is controversial. Thus, the aim of the present study is to determine the effects of virgin coconut oil ingestion, in addition to standard medications, on allergic rhinitis. We also studied the side effects of consumption of virgin coconut oil. Methods: Fifty two subjects were equally divided into test and control groups. All subjects received a daily dose of 10mg of loratadine for 28 days. The test group was given 10ml of virgin coconut oil three times a day in addition to loratadine. The symptoms of allergic rhinitis were scored at the beginning and end of the study. Results:, the symptom score were divided into nasal and non-nasal symptom scores. Sneezing score showed a significant difference, however the score was more in control group than test group, indicating that improvement in symptom was more in control group. The rest of the nasal symptom and non-nasal symptom score showed no significant difference between test and control groups. Approximately 58% of the test subjects developed side effects from consumption of virgin coconut oil, mainly gastrointestinal side effects. Conclusion: In the present study, ingestion of virgin coconut oil does not improve the overall and individual symptoms of allergic rhinitis, furthermore it has side effects.

Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Roghieh Safari ◽  
Seyed Hossein Hoseinifar ◽  
Maryam Dadar ◽  
Hien Van Doan
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
Malic Acid ◽  
Control Group ◽  
Skin Mucus ◽  
Antioxidant Genes ◽  
Treatment Comparison ◽  
Genes Expression ◽  
Significant Difference ◽  
And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

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