Collagen Formation and Wound Contraction during Repair of Small Excised Wounds in the Skin of Rats

Development ◽  
1954 ◽  
Vol 2 (3) ◽  
pp. 264-274
Author(s):  
M. Abercrombie ◽  
M. H. Flint ◽  
D. W. James
Keyword(s):  
Tensile Strength ◽  
Wound Contraction ◽  
Skin Wounds ◽  
Open Wound ◽  
Histological Studies ◽  
Collagen Formation ◽  
Wound Size ◽  
Wounded Area ◽  
Centripetal Movement ◽  
Surrounding Skin

During the healing of an open wound in the loose skin of a mammal there occurs a conspicuous diminution of wound size, the result of a centripetal movement of the whole thickness of the surrounding skin, with a corresponding shrinkage of the wound content. This ‘wound contraction’ is distinct from the process of epithelialization. Many authors (e.g. v. Gaza, 1918; Lindquist, 1946) have supposed that wound contraction is in some way causally related to the process of collagen formation, which is simultaneously occurring in the wounded area. The investigation of this supposed relation requires a more exact quantitative picture of collagen formation in skin wounds than is at present available from histological studies (such as those of Dann, Glücksmann, & Tansley, 1941; Hunt, 1941; Lindquist, 1946) or from investigations of changes in tensile strength (such as those of Howes, Sooy, & Harvey, 1929; Botsford, 1941).

Wound Contraction in Relation to Collagen Formation in Scorbutic Guinea-pigs

Development ◽  
1956 ◽  
Vol 4 (2) ◽  
pp. 167-175
Author(s):  
M. Abercrombie ◽  
M. H. Flint ◽  
D. W. James
Keyword(s):  
Ascorbic Acid ◽  
Guinea Pigs ◽  
Wound Contraction ◽  
Skin Wounds ◽  
Collagen Fibres ◽  
Collagen Formation ◽  
Effective Force ◽  
Mobile Part ◽  
Centripetal Movement

A Wound in any mobile part of the skin of a mammal diminishes in area as it heals by a centripetal movement of the undamaged skin surrounding it. This movement, usually called wound contraction, depends on a pull exerted by the material within the wound (Lindquist, 1946; Abercrombie, Flint, & James, 1954; Billingham & Medawar, 1955). It is commonly believed that the effective force is developed by the newly formed collagen fibres. In a previous paper, however (Abercrombie, Flint, & James, 1954), we found that the course of the contraction of skin wounds in rats did not parallel the deposition of new collagen, chemically measured. This result, while certainly in no way conclusive by itself, suggested that the supposed role of collagen in contraction ought to be tested more stringently. This we have now done by measuring wounds made on guinea-pigs receiving a diet devoid of ascorbic acid.

Study of traditional methods of care on the tensile strength of skin wounds in rats

1971 ◽  
Vol 122 (1) ◽  
pp. 78-80 ◽  
Author(s):  
Frederick O. Stephens ◽  
Thomas K. Hunt ◽  
J.Englebert Dunphy
Keyword(s):  
Tensile Strength ◽  
Skin Wounds ◽  
Traditional Methods

EVALUATION OF EXCISIONAL AND INCISIONAL WOUND HEALING ACTIVITY OF ELECTROHOMEOPATHIC DRUG (SPAGYRIC ESSENCE) GREEN ELECTRICITY IN RATS

2020 ◽  
pp. 72-75
Author(s):  
PRASANT KUMAR SABAT ◽  
SWETA PRIYADARSINI PRADHAN ◽  
RANDEEP PATRO
Keyword(s):  
Wound Healing ◽  
Tensile Strength ◽  
Wound Contraction ◽  
Control Group ◽  
Albino Rats ◽  
Green Electricity ◽  
Wound Model ◽  
Wound Protection ◽  
Incision Wound ◽  
Wound Healing Activity

Objective: The current research is an attempt to evaluate the wound healing activity of Electrohomeopathic drug Green electricity (GE) in Wistar rats. No data have been reported till date on the wound healing effect of GE. Hence the present research focuses on the scientific investigation of the wound healing activity of GE in rats. Methods: Excision and incision wounds were inflicted upon albino rats. In the excision wound model, the wound contraction, Scar residue, area and time of complete epithelization were measured subsequently on day 0, 3,6,9,12,15, 18 and 21 after inflicting the wound. Wound contraction was calculated as a percentage change in the initial wound size. In the incision wound model, the tensile strength was measured on the 10th post wounding day. Results: It was noted that the effect produced by the GE Showed significant wound healing (p<0.01) in all wound models when compared to the control group. In the case of the excision model, GE statistically increases the wound contraction rate and in the case of the incision model GE showed high tensile strength as compared to the control group. In both cases the epithelization period was significantly decreased as compared to control. Almost 100% wound protection was achieved by GE in 21 d as compared to control. Conclusion: GE (ointment) showed significant wound healing activity (p<0.01) in both the excised wound model and incision wound model in rats.

Low ceruloplasmin levels during recovery from major burn injury: Influence of open wound size and copper supplementation

Nutrition ◽  
1996 ◽  
Vol 12 (2) ◽  
pp. 83-88 ◽  
Author(s):  
John J. Cunningham ◽  
Martha K. Lydon ◽  
Rene Emerson ◽  
Paul R. Harmatz
Keyword(s):  
Burn Injury ◽  
Open Wound ◽  
Wound Size ◽  
Copper Supplementation ◽  
Major Burn

Postsurgical Administration of Estradiol Benzoate Decreases Tensile Strength of Healing Skin Wounds in Ovariectomized Rats

2008 ◽  
Vol 147 (1) ◽  
pp. 117-122 ◽  
Author(s):  
Peter Gál ◽  
Tomáš Toporcer ◽  
Boris Vidinský ◽  
Michal Mokrý ◽  
Tomáš Grendel ◽  
...  
Keyword(s):  
Tensile Strength ◽  
Estradiol Benzoate ◽  
Ovariectomized Rats ◽  
Skin Wounds

The recruitment of bone marrow-derived cells to skin wounds is independent of wound size

2011 ◽  
Vol 19 (2) ◽  
pp. 260-267 ◽  
Author(s):  
Jochem Verstappen ◽  
Christos Katsaros ◽  
Anne Marie Kuijpers-Jagtman ◽  
Ruurd Torensma ◽  
Johannes W. Von den Hoff
Keyword(s):  
Bone Marrow ◽  
Skin Wounds ◽  
Wound Size ◽  
Bone Marrow Derived Cells

Effects of hyaluronan and iodine on wound contraction and granulation tissue formation in rat skin wounds

2010 ◽  
Vol 35 (4) ◽  
pp. 373-379 ◽  
Author(s):  
R. Slavkovsky ◽  
R. Kohlerova ◽  
A. Jiroutova ◽  
M. Hajzlerova ◽  
L. Sobotka ◽  
...  
Keyword(s):  
Granulation Tissue ◽  
Wound Contraction ◽  
Skin Wounds ◽  
Tissue Formation ◽  
Rat Skin ◽  
Granulation Tissue Formation

scholarly journals Triticum aestivum in open skin wounds: cytotoxicity and collagen histopathology

2018 ◽  
Vol 39 (4) ◽  
pp. 1547
Author(s):  
Mariana Teixeira Tillmann ◽  
Cláudia Beatriz De Mello Mendes ◽  
Geferson Fischer ◽  
Antonio Sergio Varela Júnior ◽  
Cristina Gevehr Fernandes ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Control Cell ◽  
Negative Control ◽  
In Vitro Test ◽  
Skin Wounds ◽  
In Vivo Test ◽  
Collagen Formation

Phytoterapic compounds have been used in wound healing for many centuries. Nowadays, scientific evidences of phytotherapeutics is a requirement of the legislation. The scientific literature notes the need for healing topics yielding scars that are both aesthetically appealing and resistant. We aimed to evaluate the cytotoxicity of several doses of T. aestivum extract (2 mg mL-1, 4 mg mL-1, 6 mg mL-1, 8 mg mL-1 and 10 mg mL-1) in a fibroblast cell line and the healing process in an in vivo experimental model (New Zealand rabbits). For this, MTT test in 3T6 cells was performed in duplicates using MEM (0 mg ml-1) as negative control. Cell viability was calculated as: absorbance average in treatments/absorbance average in controls x 100. In vivo test was performed in 78 skin wounds in rabbits that were treated with 2 mg ml-1and 10 mg ml-1 of T. aestivum and non-ionic cream for 21 days. After this period, it was evaluated the histology using picrosorius and Gomori’s trichrome staining. Statistical analysis was evaluated using T test (Graphpad) for cytotoxicity assay, Fischer test for the gomori trichrome test (Grahpad) and Kruskal-Wallis (Statistic 9.0) for picrosirius test. The in vitro test resulted in cytotoxicity observed at 2mg mL-1 whereas cells were viable at higher doses. On the other hand, it was observed that collagen formation of wounds was more uniform with this dose than with 10mg mL-1 extract in the in vivo study. Thus, we conclude that the 2mg mL-1 T. aestivum aqueous extract dose was more efficient in the in vivo wound healing study, despite its cytotoxic effects in vitro.

scholarly journals WOUND HEALING AND COLLAGEN FORMATION

1962 ◽  
Vol 12 (3) ◽  
pp. 533-551 ◽  
Author(s):  
Russell Ross ◽  
Earl P. Benditt
Keyword(s):  
Electron Microscopy ◽  
Wound Healing ◽  
Protein Synthesis ◽  
Small Groups ◽  
Guinea Pigs ◽  
Light And Electron Microscopy ◽  
Dense Matter ◽  
Skin Wounds ◽  
Intercellular Spaces ◽  
Collagen Formation

The sequence encountered in healing skin wounds in scorbutic guinea pigs has been examined by methods of light and electron microscopy. Linear incisions in the skin of female guinea pigs fed a scorbutigenic diet were allowed to heal. The wounds were removed for examination at 1, 3, 5, 9, and 14 days after wounding. The fibroblasts of the scorbutic wounds differ from those of the controls in three major aspects. First, little collagen is present within the intercellular spaces, although small groups of individual collagen fibrils can be found adjacent to some of the fibroblasts; in addition, large amounts of somewhat fibrillar, poorly structured, dense matter are present throughout the extracellular regions. The second alteration consists of large aggregates of intracytoplasmic lipid deposits present within the majority of the fibroblasts. Third, the endoplasmic reticulum of the fibroblasts is altered in form from that of the controls. The profiles of the cisternae are round, non-continuous within the plane of section, and are less extensively developed than in the controls. An increased macrophagic activity of the histiocytes is also described. These changes are discussed in light of the available biochemical data associated with this abnormality of protein synthesis.

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