scholarly journals The combined action of Esrrb and Nr5a2 is essential for murine naïve pluripotency.

Development ◽  
2021 ◽  
Author(s):  
Nicola Festuccia ◽  
Nick Owens ◽  
Almira Chervova ◽  
Agnès Dubois ◽  
Pablo Navarro
Keyword(s):  
Nuclear Receptors ◽  
Embryonic Stem ◽  
Regulatory Elements ◽  
Culture Conditions ◽  
The Self ◽  
Interconnected Network ◽  
Orphan Nuclear Receptors ◽  
Self Renewal ◽  
Two Factors ◽  
Combined Action

The maintenance of pluripotency in mouse embryonic stem cells (ESCs) is governed by the action of an interconnected network of transcription factors. Among them, only Oct4 and Sox2 have been shown to be strictly required for the self-renewal of ESCs and pluripotency, particularly in culture conditions where differentiation cues are chemically inhibited. Here, we report that the conjunct activity of two orphan nuclear receptors, Esrrb and Nr5a2, parallels the importance of that of Oct4 and Sox2 in naïve ESCs. By occupying a large common set of regulatory elements, these two factors control the binding of Oct4, Sox2 and Nanog to DNA. Consequently, in their absence the pluripotency network collapses and the transcriptome is substantially deregulated, leading to the differentiation of ESCs. Altogether, this work identifies orphan nuclear receptors, previously thought to be performing supportive functions, as a new set of core regulators of naïve pluripotency.

scholarly journals The combined action of Esrrb and Nr5a2 is essential for naïve pluripotency

2020 ◽  
Author(s):  
Nicola Festuccia ◽  
Nick Owens ◽  
Almira Chervova ◽  
Agnès Dubois ◽  
Pablo Navarro
Keyword(s):  
Nuclear Receptors ◽  
Embryonic Stem ◽  
Regulatory Elements ◽  
Culture Conditions ◽  
The Self ◽  
Interconnected Network ◽  
Orphan Nuclear Receptors ◽  
Self Renewal ◽  
Two Factors ◽  
Combined Action

The maintenance of pluripotency in mouse embryonic stem cells (ESCs) is governed by the action of an interconnected network of transcription factors. Among them, only Oct4 and Sox2 have been shown to be strictly required for the self-renewal of ESCs and pluripotency, particularly in culture conditions where differentiation cues are chemically inhibited. Here, we report that the conjunct activity of two orphan nuclear receptors, Esrrb and Nr5a2, parallels the importance of that of Oct4 and Sox2 in naïve ESCs. By occupying a large common set of regulatory elements, these two factors control the binding of Oct4, Sox2 and Nanog to DNA. Consequently, in their absence the pluripotency network collapses and the transcriptome is substantially deregulated, leading to the differentiation of ESCs. Altogether, this work identifies orphan nuclear receptors, previously thought to be performing supportive functions, as a new set of core regulators of naïve pluripotency.

scholarly journals Musashi2 Is Required for the Self-Renewal and Pluripotency of Embryonic Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34827 ◽  
Author(s):  
Erin L. Wuebben ◽  
Sunil K. Mallanna ◽  
Jesse L. Cox ◽  
Angie Rizzino
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
The Self ◽  
Self Renewal

scholarly journals Comprehensive Identification of Krüppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150715 ◽  
Author(s):  
Hyojung Jeon ◽  
Tsuyoshi Waku ◽  
Takuya Azami ◽  
Le Tran Phuc Khoa ◽  
Jun Yanagisawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Family Members ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cellular Reprogramming ◽  
The Self ◽  
Self Renewal

Optimization of Culture Conditions to Support Long-Term Self-Renewal of Buffalo (Bubalus bubalis) Embryonic Stem Cell-Like Cells

2011 ◽  
Vol 13 (6) ◽  
pp. 539-549 ◽  
Author(s):  
Ruchi Sharma ◽  
Aman George ◽  
Nitin Manchindra Kamble ◽  
Karn Pratap Singh ◽  
Manmohan Singh Chauhan ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Bubalus Bubalis ◽  
Culture Conditions ◽  
Self Renewal ◽  
Optimization Of Culture Conditions

Transcription factors that behave as master regulators during mammalian embryogenesis function as molecular rheostats

2008 ◽  
Vol 411 (2) ◽  
pp. e5-e7 ◽  
Author(s):  
Angie Rizzino
Keyword(s):  
Transcription Factors ◽  
Molecular Mechanisms ◽  
Es Cells ◽  
Embryonic Stem ◽  
Special Property ◽  
The Self ◽  
Leukaemia Inhibitory Factor ◽  
Self Renewal ◽  
Master Regulators ◽  
Mammalian Embryogenesis

Three transcription factors, Sox2, Oct-3/4 and Nanog, have been identified as master regulators that orchestrate mammalian embryogenesis as well as the self-renewal and pluripotency of ES (embryonic stem) cells. Efforts to understand how these transcription factors function have shown that they have a special property in common. Small changes in the expression of any one of these factors dramatically alter the self-renewal and pluripotency of ES cells. In this way, each functions as a molecular rheostat to control the behaviour of ES cells. Recent studies have begun to examine the molecular mechanisms that regulate the levels of these transcription factors. In this issue of the Biochemical Journal, Mullin and co-workers report that Nanog can self-associate to form dimers. Importantly, they also show that the domain responsible for dimerization is also needed for Nanog to sustain the self-renewal of ES cells in the absence of the cytokine LIF (leukaemia inhibitory factor). On the basis of their studies, they propose a novel mechanism for regulating the interactions between Nanog and other nuclear proteins.

Cdk1 is required for the self-renewal of mouse embryonic stem cells

2011 ◽  
Vol 112 (3) ◽  
pp. 942-948 ◽  
Author(s):  
Wei Wei Zhang ◽  
Xiao Jie Zhang ◽  
Hui Xian Liu ◽  
Jie Chen ◽  
Yong Hong Ren ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
The Self ◽  
Self Renewal
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