The hedgehog gene family in Drosophila and vertebrate development

Development ◽  
1994 ◽  
Vol 1994 (Supplement) ◽  
pp. 43-51
Author(s):  
Michael J. Fietz ◽  
Jean-Paul Concordet ◽  
Robert Barbosa ◽  
Randy Johnson ◽  
Stefan Krauss ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Gene Family ◽  
Sonic Hedgehog ◽  
Cell Patterning ◽  
Vertebrate Development ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

The segment polarity gene hedgehog plays a central role in cell patterning during embryonic and post-embryonic development of the dipteran, Drosophila melanogaster. Recent studies have identified a family of hedgehog related genes in vertebrates; one of these, Sonic hedgehog is implicated in positional signalling processes that show interesting similarities with those controlled by its Drosophila homologue.

Molecular organisation and expression pattern of the segment polarity gene fused of Drosophila melanogaster

1993 ◽  
Vol 44 (1) ◽  
pp. 65-80 ◽  
Author(s):  
P THEROND ◽  
D BUSSON ◽  
E GUILLEMET ◽  
B LIMBOURGBOUCHON ◽  
T PREAT ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Expression Pattern ◽  
Segment Polarity ◽  
Molecular Organisation ◽  
Segment Polarity Gene ◽  
Polarity Gene

Molecular cloning of fused, a gene required for normal segmentation in the Drosophila melanogaster embryo

1987 ◽  
Vol 7 (9) ◽  
pp. 3244-3251
Author(s):  
M C Mariol ◽  
T Preat ◽  
B Limbourg-Bouchon
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Dna ◽  
Developmental Stages ◽  
Developmental Expression ◽  
Segment Polarity ◽  
D Region ◽  
Fused Gene ◽  
Phenotypic Rescue ◽  
Segment Polarity Gene ◽  
Polarity Gene

Using the chromosomal walk technique, we isolated recombinant lambda bacteriophage and cosmid clones spanning 250 kilobases (kb) in the 17C-D region of the X chromosome of Drosophila melanogaster. This region was known to contain the segment polarity gene fused. Several lethal fused mutations were used to define more precisely the localization of this locus. Southern analysis of genomic DNA revealed that all of them were relatively large deficiencies, the smallest one being 40 kb long. None of the 12 viable fused mutations examined possessed detectable alterations. We isolated a cosmid containing an insertion covering the entire smallest fused deletion (40 kb). We injected this DNA into fused mutant embryos and obtained a partial phenotypic rescue of the embryonic pattern, indicating that this region contained all the sequences necessary for the embryonic expression of the fu+ gene. Within this DNA, a subclone of 14 kb codes for poly(A)+ RNAs of 3.5, 2.5, 1.6, and 1.3 kb detected in embryos from various developmental stages as well as in adults. All these transcripts showed the same developmental expression. This transcribed region was injected into fused mutant embryos, and once again we obtained a partial rescue of the embryonic phenotype, confirming that this region contained at least the fused gene.

scholarly journals The hereditary renal cell carcinoma 3;8 translocation fuses FHIT to a patched-related gene, TRC8

1998 ◽  
Vol 95 (16) ◽  
pp. 9572-9577 ◽  
Author(s):  
Robert M. Gemmill ◽  
James D. West ◽  
Ferenc Boldog ◽  
Naotake Tanaka ◽  
Linda J. Robinson ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Sonic Hedgehog ◽  
Renal Cell ◽  
Chromosomal Translocation ◽  
Coding Region ◽  
Segment Polarity ◽  
Membrane Spanning ◽  
Segment Polarity Gene ◽  
Polarity Gene

The 3;8 chromosomal translocation, t(3;8)(p14.2;q24.1), was described in a family with classical features of hereditary renal cell carcinoma. Previous studies demonstrated that the 3p14.2 breakpoint interrupts the fragile histidine triad gene (FHIT) in its 5′ noncoding region. However, evidence that FHIT is causally related to renal or other malignancies is controversial. We now show that the 8q24.1 breakpoint region encodes a 664-aa multiple membrane spanning protein, TRC8, with similarity to the hereditary basal cell carcinoma/segment polarity gene, patched. This similarity involves two regions of patched, the putative sterol-sensing domain and the second extracellular loop that participates in the binding of sonic hedgehog. In the 3;8 translocation, TRC8 is fused to FHIT and is disrupted within the sterol-sensing domain. In contrast, the FHIT coding region is maintained and expressed. In a series of sporadic renal carcinomas, an acquired TRC8 mutation was identified. By analogy to patched, TRC8 might function as a signaling receptor and other pathway members, to be defined, are mutation candidates in malignant diseases involving the kidney and thyroid.

scholarly journals Characterization of Suppressor of fused, a complete suppressor of the fused segment polarity gene of Drosophila melanogaster.

Genetics ◽  
1992 ◽  
Vol 132 (3) ◽  
pp. 725-736 ◽  
Author(s):  
T Préat
Keyword(s):  
Drosophila Melanogaster ◽  
Maternal Effect ◽  
Opposite Effect ◽  
Loss Of Function ◽  
Suppressor Of Fused ◽  
Segment Polarity ◽  
New Gene ◽  
Segment Polarity Gene ◽  
Polarity Gene

Abstract fused (fu) is a maternal effect segment polarity gene of Drosophila melanogaster. In addition, fu females have tumorous ovaries. Two ethyl methanesulfonate mutageneses were carried out in order to isolate suppressors of the fu phenotype. A new gene, Suppressor of fused (Su(fu)), was identified. It is located in the 87C8 region of the third chromosome. Su(fu) displays a maternal effect and is also expressed later in development. Although Su(fu)LP is a complete loss-of-function mutation, it is homozygous viable and has no phenotype by itself. Su(fu) fully suppresses the embryonic and adult phenotypes of fu mutants. Su(fu) mutations are semidominant and a Su(fu)+ duplication has an opposite effect, enhancing the fused phenotype. It is proposed therefore that the Su(fu)+ product is involved in the same developmental step as the Fu+ kinase. Thus, a new gene interacting with the segment polarity pathway was identified using an indirect approach.

Polyembryonic development: insect pattern formation in a cellularized environment

Development ◽  
1996 ◽  
Vol 122 (3) ◽  
pp. 795-804 ◽  
Author(s):  
M. Grbic ◽  
L.M. Nagy ◽  
S.B. Carroll ◽  
M. Strand
Keyword(s):  
Drosophila Melanogaster ◽  
Regulatory Proteins ◽  
Homeotic Genes ◽  
Axial Patterning ◽  
Copidosoma Floridanum ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Pair Rule ◽  
Egg Polarity ◽  
Polarity Gene

THe polyembryonic wasp Copidosoma floridanum produces up to 2000 individuals from a single egg. During the production of individual embryos the original anteroposterior axis of the egg is lost and axial patterning must subsequently be reestablished within each embryo. The mechanism by which this occurs is unknown. In most insects, egg polarity is established during oogenesis and early development takes place in a syncytium. In Drosophila melanogaster, the syncytium is considered essential for establishing the morphogenetic gradients that initiate segmental patterning. However, we found that development of C. floridanum occurs almost exclusively in a cellularized environment. To determine whether the D. melanogaster patterning cascade is conserved in the absence of a syncytium, we analyzed the expression of Even-skipped, Engrailed and Ultrabithorax/Abdominal-A during polyembryonic development. Here we show that in spite of the absence of a syncytium, the elements of the D. melanogaster segmentation hierarchy are conserved. The segment-polarity gene Engrailed and the homeotic genes Ultrabithorax/Abdominal-A are expressed in a conserved pattern relative to D. melanogaster. However, we detect an alteration in the expression of the Even-skipped antigen. Even-skipped is initially expressed in segmentally reiterated stripes and not in the pair-rule pattern as it is in D. melanogaster. We also observe that the expression of these regulatory proteins does not occur during the early proliferative phases of polyembryony. Our results indicate that a syncytium is not required for segmental patterning in this insect.

scholarly journals Molecular cloning of fused, a gene required for normal segmentation in the Drosophila melanogaster embryo.

1987 ◽  
Vol 7 (9) ◽  
pp. 3244-3251 ◽  
Author(s):  
M C Mariol ◽  
T Preat ◽  
B Limbourg-Bouchon
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Dna ◽  
Developmental Stages ◽  
Developmental Expression ◽  
Segment Polarity ◽  
D Region ◽  
Fused Gene ◽  
Phenotypic Rescue ◽  
Segment Polarity Gene ◽  
Polarity Gene

Using the chromosomal walk technique, we isolated recombinant lambda bacteriophage and cosmid clones spanning 250 kilobases (kb) in the 17C-D region of the X chromosome of Drosophila melanogaster. This region was known to contain the segment polarity gene fused. Several lethal fused mutations were used to define more precisely the localization of this locus. Southern analysis of genomic DNA revealed that all of them were relatively large deficiencies, the smallest one being 40 kb long. None of the 12 viable fused mutations examined possessed detectable alterations. We isolated a cosmid containing an insertion covering the entire smallest fused deletion (40 kb). We injected this DNA into fused mutant embryos and obtained a partial phenotypic rescue of the embryonic pattern, indicating that this region contained all the sequences necessary for the embryonic expression of the fu+ gene. Within this DNA, a subclone of 14 kb codes for poly(A)+ RNAs of 3.5, 2.5, 1.6, and 1.3 kb detected in embryos from various developmental stages as well as in adults. All these transcripts showed the same developmental expression. This transcribed region was injected into fused mutant embryos, and once again we obtained a partial rescue of the embryonic phenotype, confirming that this region contained at least the fused gene.

Genomic organization of the segment polarity gene pan in Drosophila melanogaster

1998 ◽  
Vol 258 (1-2) ◽  
pp. 45-52 ◽  
Author(s):  
D. Dooijes ◽  
M. van Beest ◽  
M. van de Wetering ◽  
G. Boulanger ◽  
T. Jones ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Genomic Organization ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

scholarly journals oroshigane, a New Segment Polarity Gene of Drosophila melanogaster, Functions in Hedgehog Signal Transduction

Genetics ◽  
1997 ◽  
Vol 145 (4) ◽  
pp. 1041-1052
Author(s):  
Janet L Epps ◽  
Jessa B Jones ◽  
Soichi Tanda
Keyword(s):  
Signal Transduction ◽  
Drosophila Melanogaster ◽  
Dependent Relationship ◽  
Embryonic Lethal ◽  
Segment Polarity ◽  
Eye Imaginal Disc ◽  
Dose Dependent ◽  
Segment Polarity Gene ◽  
Dominant Suppressor ◽  
Polarity Gene

Here we describe a new segment polarity gene of Drosophila melanogaster, oroshigane (oro). Identified as a dominant enhancer of Bar (B), oro is also recessive embryonic lethal, and homozygous oro embryos show variable substitution of naked cuticle with denticles. These patterns are distinctly similar to those of hedgehog (hh) and wingless (wg) embryos, which indicates that oro functions in determining embryonic segment polarity. Evidence that oro function is involved in Hh signal transduction during embryogenesis is provided by its genetic interactions with the segment polarity genes patched (ptc) and fused (fu). Furthermore, ptcIN is a dominant suppressor of the oro embryonic lethal phenotype, suggesting a close and dose-dependent relationship between oro and ptc in Hh signal transduction. oro function is also required in imaginal development. The oro1 allele significantly reduces decapentaplegic (dpp), but not hh, expression in the eye imaginal disc. Furthermore, oro enhances the fu1 wing phenotype in a dominant manner. Based upon the interactions of oro with hh, ptc, and fu, we propose that the oro gene plays important roles in Hh signal transduction.

Pattern formation in Drosophila head development: the role of the orthodenticle homeobox gene

Development ◽  
1995 ◽  
Vol 121 (11) ◽  
pp. 3561-3572 ◽  
Author(s):  
J. Royet ◽  
R. Finkelstein
Keyword(s):  
Drosophila Melanogaster ◽  
Pattern Formation ◽  
Homeobox Gene ◽  
Significant Progress ◽  
Medial Head ◽  
Head Development ◽  
Segment Polarity ◽  
Segment Polarity Gene ◽  
Polarity Gene

Significant progress has been made towards understanding how pattern formation occurs in the imaginal discs that give rise to the limbs of Drosophila melanogaster. Here, we examine the process of regional specification that occurs in the eye-antennal discs, which form the head of the adult fruitfly. We demonstrate genetically that there is a graded requirement for the activity of the orthodenticle homeobox gene in forming specific structures of the developing head. Consistent with this result, we show that OTD protein is expressed in a graded fashion across the disc primordia of these structures and that different threshold levels of OTD are required for the formation of specific subdomains of the head. Finally, we provide evidence suggesting that otd acts through the segment polarity gene engrailed to specify medial head development.

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