scholarly journals Precise coordination of cell-ECM adhesion is essential for efficient melanoblast migration during development

Development ◽  
2020 ◽  
Vol 147 (14) ◽  
pp. dev184234 ◽  
Author(s):  
Amanda Haage ◽  
Kelsey Wagner ◽  
Wenjun Deng ◽  
Bhavya Venkatesh ◽  
Caitlin Mitchell ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Long Range ◽  
Hair Follicles ◽  
Fine Tuning ◽  
Adapter Protein ◽  
Range Cell ◽  
Range Cell Migration ◽  
Integrin Family

ABSTRACTMelanoblasts disperse throughout the skin and populate hair follicles through long-range cell migration. During migration, cells undergo cycles of coordinated attachment and detachment from the extracellular matrix (ECM). Embryonic migration processes that require cell-ECM attachment are dependent on the integrin family of adhesion receptors. Precise regulation of integrin-mediated adhesion is important for many developmental migration events. However, the mechanisms that regulate integrin-mediated adhesion in vivo in melanoblasts are not well understood. Here, we show that autoinhibitory regulation of the integrin-associated adapter protein talin coordinates cell-ECM adhesion during melanoblast migration in vivo. Specifically, an autoinhibition-defective talin mutant strengthens and stabilizes integrin-based adhesions in melanocytes, which impinges on their ability to migrate. Mice with defective talin autoinhibition exhibit delays in melanoblast migration and pigmentation defects. Our results show that coordinated integrin-mediated cell-ECM attachment is essential for melanoblast migration and that talin autoinhibition is an important mechanism for fine-tuning cell-ECM adhesion during cell migration in development.

scholarly journals Rectified directional sensing in long-range cell migration

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Akihiko Nakajima ◽  
Shuji Ishihara ◽  
Daisuke Imoto ◽  
Satoshi Sawai
Keyword(s):  
Cell Migration ◽  
Long Range ◽  
Range Cell ◽  
Range Cell Migration

Cell-extracellular matrix interactions under in vivo conditions during interstitial cell migration in Hydra vulgaris

Development ◽  
1994 ◽  
Vol 120 (2) ◽  
pp. 425-432 ◽  
Author(s):  
X. Zhang ◽  
M.P. Sarras
Keyword(s):  
Extracellular Matrix ◽  
Cell Migration ◽  
Synthetic Peptide ◽  
Interstitial Cell ◽  
Extracellular Matrix Components ◽  
Matrix Interactions ◽  
Apical Half ◽  
Matrix Components ◽  
Extracellular Matrix Interactions

Interstitial cell (I-cell) migration in hydra is essential for establishment of the regional cell differentiation pattern in the organism. All previous in vivo studies have indicated that cell migration in hydra is a result of cell-cell interactions and chemotaxic gradients. Recently, in vitro cell adhesion studies indicated that isolated nematocytes could bind to substrata coated with isolated hydra mesoglea, fibronectin and type IV collagen. Under these conditions, nematocytes could be observed to migrate on some of these extracellular matrix components. By modifying previously described hydra grafting techniques, two procedures were developed to test specifically the role of extracellular matrix components during in vivo I-cell migration in hydra. In one approach, the extracellular matrix structure of the apical half of the hydra graft was perturbed using beta-aminopropionitrile and beta-xyloside. In the second approach, grafts were treated with fibronectin, RGDS synthetic peptide and antibody to fibronectin after grafting was performed. In both cases, I-cell migration from the basal half to the apical half of the grafts was quantitatively analyzed. Statistical analysis indicated that beta-aminopropionitrile, fibronectin, RGDS synthetic peptide and antibody to fibronectin all were inhibitory to I-cell migration as compared to their respective controls. beta-xyloside treatment had no effect on interstitial cell migration. These results indicate the potential importance of cell-extracellular matrix interactions during in vivo I-cell migration in hydra.

Presenting a New Technique for Multi-Target Tracking in Inverse Synthetic Aperture Radar based on PHD Filter in the Presence of Clutters

Frequenz ◽  
2018 ◽  
Vol 72 (7-8) ◽  
pp. 391-399 ◽  
Author(s):  
Hamid Dehghani ◽  
Navid Daryasafar
Keyword(s):  
Cell Migration ◽  
Synthetic Aperture Radar ◽  
Synthetic Aperture ◽  
Moving Targets ◽  
Inverse Synthetic Aperture Radar ◽  
Probability Hypothesis Density ◽  
Implementation Phase ◽  
Range Cell ◽  
Range Cell Migration ◽  
Aperture Radar

Abstract Using Probability Hypothesis Density (PHD) filtering, a novel approach is proposed in this paper for simultaneous tracking of multiple moving targets in received data by Inverse Synthetic Aperture Radar (ISAR) system. Since PHD filtering approach is implemented successively in prediction and update steps, its performance quality will obviously be higher in “Spotlight” imaging mode than in “Stripmap”. Thus, its application to Spotlight mode is generally more logical. The idea to integrate tracking capability into ISAR system processor is to sort radar received data to correct Range Cell Migration (RCM) prior to tracking operations. Clearly, Range Cell Migration Compensation (RCMC) approach is different from this approach in image formation process, in terms of their implementation phase. However, they are implemented in a similar way. As simulation results reveal, applying Range Cell Migration Compensation to the raw data received by ISAR before tracking operation, results in high quality tracking of moving targets.

scholarly journals Focusing of Spotlight Tandem-Configuration Bistatic Data with Frequency Scaling Algorithm

2016 ◽  
Vol 2016 ◽  
pp. 1-15
Author(s):  
Shichao Chen ◽  
Ming Liu ◽  
Jun Wang ◽  
Fugang Lu ◽  
Mengdao Xing
Keyword(s):  
Cell Migration ◽  
Data Processing ◽  
High Precision ◽  
Frequency Scaling ◽  
Tandem Configuration ◽  
Imaging Algorithm ◽  
Bistatic Sar ◽  
Range Cell ◽  
Sar Data ◽  
Range Cell Migration

A frequency scaling (FS) imaging algorithm is proposed for spotlight bistatic SAR data processing. Range cell migration correction (RCMC) is realized through phase multiplication. The proposed algorithm is insensitive to the length of the baseline due to the high precision of the point target (PT) spectrum that we are based on. It is capable of handling bistatic SAR data with a large baseline to range ratio. The algorithms suitable for small and high squint angles are both discussed according to whether the range dependence of the second range compression (SRC) can be neglected or not. Simulated experiments validate the effectiveness of the proposed algorithm.

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