scholarly journals A genetic system for tissue-specific inhibition of cell proliferation

Development ◽  
2020 ◽  
Vol 147 (4) ◽  
pp. dev183830 ◽  
Author(s):  
Wenjuan Pu ◽  
Ximeng Han ◽  
Lingjuan He ◽  
Yan Li ◽  
Xiuzhen Huang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Genetic System ◽  
Specific Inhibition ◽  
Tissue Specific

Tissue-specific inhibition of cell proliferation in Ehrlich's ascites tumor

1976 ◽  
Vol 82 (6) ◽  
pp. 1846-1848
Author(s):  
Yu. A. Romanov ◽  
V. P. Rybakov ◽  
A. I. Antokhin ◽  
V. P. Barysheva
Keyword(s):  
Cell Proliferation ◽  
Specific Inhibition ◽  
Ascites Tumor ◽  
Ehrlich's Ascites Tumor ◽  
Tissue Specific

scholarly journals Transgenic Approaches for Cyanogen Reduction in Cassava

2007 ◽  
Vol 90 (5) ◽  
pp. 1450-1455 ◽  
Author(s):  
Dimuth Siritunga ◽  
Richard Sayre
Keyword(s):  
Gene Expression ◽  
Potential Benefit ◽  
Fold Increase ◽  
Selective Inhibition ◽  
Alternative Strategy ◽  
Specific Inhibition ◽  
Cyanogenic Glycoside ◽  
Wild Type ◽  
Hydroxynitrile Lyase ◽  
Tissue Specific

Abstract For cassava to become a safe and acceptable crop, it is necessary to reduce the cyanogen levels in cassava foods. While this objective can be achieved by processing procedures, recent findings have shown that it is also possible to achieve it by suppression of cyanogen synthesis or by accelerating cyanogen turnover and volatilization. In 2003, cyanogen-free cultivars were generated by selective inhibition CYP79D1/D2 gene expression. The CYP79D1/D2 enzymes catalyze the first-dedicated step in cyanogen synthesis. Tissue-specific inhibition of CYP79D1/D2 expression in leaves lead to a 99 reduction in root cyanogen levels, indicating that the cyanogenic glycoside, linamarin, is synthesized in leaves and transported to roots. An alternative strategy to the reduce cyanogen content is to enhance cyanogen detoxification and cyanide volatilization during processing. This strategy has the advantage that cyanogen levels in unprocessed roots are not altered, potentially providing protection against herbivory and/or theft. To produce cultivars that promote rapid cyanide volatilization, hydroxynitrile lyase (HNL), which catalyzes the last step in cyanogenesis, was overexpressed in roots. Elevated HNL activity resulted in a 3-fold increase in the rate of cyanogen turnover. Importantly, the cyanogen content of the transformed and wild-type plants was identical, a potential benefit for farmers.

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