scholarly journals Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development

Development ◽  
2019 ◽  
Vol 146 (12) ◽  
pp. dev173476 ◽  
Author(s):  
Guang Li ◽  
Lei Tian ◽  
William Goodyer ◽  
Eric J. Kort ◽  
Jan W. Buikema ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Expression Analysis ◽  
Heart Development ◽  
Cycle Dependent ◽  
Cell Expression

scholarly journals Correction: Single cell expression analysis reveals anatomical and cell cycle-dependent transcriptional shifts during heart development

Development ◽  
2020 ◽  
Vol 147 (7) ◽  
pp. dev190819
Author(s):  
Guang Li ◽  
Lei Tian ◽  
William Goodyer ◽  
Eric J. Kort ◽  
Jan W. Buikema ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Expression Analysis ◽  
Heart Development ◽  
Cycle Dependent ◽  
Cell Expression

On-Chip Cell Sorter for Single Cell Expression Analysis

2001 ◽  
pp. 271-273 ◽  
Author(s):  
T. Ichiki ◽  
T. Ujiie ◽  
T. Hara ◽  
Y. Horiike ◽  
K. Yasuda
Keyword(s):  
Single Cell ◽  
Expression Analysis ◽  
Cell Sorter ◽  
On Chip ◽  
Cell Expression

scholarly journals Single-cell expression analysis of BMP15 and GDF9 in mature oocytes and BMPR2 in cumulus cells of women with polycystic ovary syndrome undergoing controlled ovarian hyperstimulation

2012 ◽  
Vol 29 (10) ◽  
pp. 1057-1065 ◽  
Author(s):  
Luciana Ochuiuto Teixeira de Resende ◽  
Alessandra Aparecida Vireque ◽  
Laura Ferreira Santana ◽  
Daniel Antunes Moreno ◽  
Ana Carolina Japur de Sá Rosa e Silva ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Single Cell ◽  
Expression Analysis ◽  
Polycystic Ovary ◽  
Cumulus Cells ◽  
Controlled Ovarian Hyperstimulation ◽  
Ovarian Hyperstimulation ◽  
Ovary Syndrome ◽  
Cell Expression

Statistical single-cell analysis of cell cycle-dependent quantum dot cytotoxicity and cellular uptake using a microfluidic system

RSC Advances ◽  
2014 ◽  
Vol 4 (47) ◽  
pp. 24929-24934 ◽  
Author(s):  
Jing Wu ◽  
Haifang Li ◽  
Qiushui Chen ◽  
Xuexia Lin ◽  
Wu Liu ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Quantum Dot ◽  
Single Cell ◽  
Cellular Uptake ◽  
Single Cell Analysis ◽  
Single Cells ◽  
Microfluidic System ◽  
Cell Analysis ◽  
System P ◽  
Cycle Dependent

The response of single cells in different cell cycle phases to QD cytotoxicity studied on a microfluidic device.

scholarly journals Single-cell transcriptomes of fluorescent, ubiquitination-based cell cycle indicator cells

2016 ◽  
Author(s):  
Michael Böttcher ◽  
Tsukasa Kouno ◽  
Elo Madissoon ◽  
Efthymios Motakis ◽  
Imad Abugessaisa ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Single Cell ◽  
Single Cells ◽  
Open Data ◽  
Image Data ◽  
Sequencing Data ◽  
Fluorescence Measurements ◽  
Starting Point ◽  
Public Repositories ◽  
Cell Expression

AbstractWe used a transgenic HeLa cell line that reports cell cycle phases through fluorescent, ubiquitination-based cell cycle indicators (Fucci), to produce a reference dataset of more than 270 curated single cells. Microscopic images were taken from each cell followed by RNA-sequencing, so that single-cell expression data is associated to the fluorescence intensity of the Fucci probes in the same cell. We developed an open data management and quality control workflow that enables users to replicate the processing of the sequence and microscopic image data that we deposited in public repositories. The workflow outputs a table with metadata, that is the starting point for further studies on these data. Beyond its use for cell cycle studies, We also expect that our workflow can be adapted to other single-cell projects using a similar combination of sequencing data and fluorescence measurements.

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