scholarly journals Stochastic single cell migration leads to robust horizontal cell layer formation in the vertebrate retina

Development ◽  
2019 ◽  
Vol 146 (12) ◽  
pp. dev173450 ◽  
Author(s):  
Rana Amini ◽  
Anastasia A. Labudina ◽  
Caren Norden
Keyword(s):  
Cell Migration ◽  
Single Cell ◽  
Cell Layer ◽  
Horizontal Cell ◽  
Layer Formation ◽  
Vertebrate Retina

scholarly journals Stochastic Single Cell Behaviour Leads to Robust Horizontal Cell Layer Formation in the Vertebrate Retina

2019 ◽  
Author(s):  
Rana Amini ◽  
Anastasia Labudina ◽  
Caren Norden
Keyword(s):  
Single Cell ◽  
Cell Layer ◽  
Horizontal Cell ◽  
Light Sheet ◽  
Cell Dynamics ◽  
Cell Behaviour ◽  
Vertebrate Retina ◽  
Laminar Pattern ◽  
Cell Cycle Dynamics

ABSTRACTDevelopmental programs that arrange cells and tissues into patterned organs are remarkably robust. In the developing vertebrate retina for example, neurons reproducibly assemble into distinct layers giving the mature organ its overall structured appearance. This stereotypic neuronal arrangement, termed lamination, is important for efficient neuronal connectivity. While retinal lamination is conserved in many vertebrates including humans, how it emerges from single cell behaviour is not fully understood. To shed light on this question, we here investigated the formation of the retinal horizontal cell layer. Using in vivo light sheet imaging of the developing zebrafish retina, we generated a comprehensive quantitative analysis of horizontal single cell behaviour from birth to final positioning. Interestingly, we find that all parameters analyzed including cell cycle dynamics, migration paths and kinetics as well as sister cell dispersal are very heterogeneous. Thus, horizontal cells show individual non-stereotypic behaviour before final positioning. Yet, these initially stochastic cell dynamics always generate the correct laminar pattern. Consequently, our data shows that lamination of the vertebrate retina contains a yet underexplored extent of single cell stochasticity.

scholarly journals Nonapical Symmetric Divisions Underlie Horizontal Cell Layer Formation in the Developing Retina In Vivo

Neuron ◽  
2007 ◽  
Vol 56 (4) ◽  
pp. 597-603 ◽  
Author(s):  
Leanne Godinho ◽  
Philip R. Williams ◽  
Yvonne Claassen ◽  
Elayne Provost ◽  
Steven D. Leach ◽  
...  
Keyword(s):  
Cell Layer ◽  
Horizontal Cell ◽  
Layer Formation

scholarly journals Mechanical stimulation promotes enthesis injury repair by mobilizing Prx1+ cells via ciliary TGF-β signaling

2021 ◽  
Author(s):  
Han Xiao ◽  
Tao Zhang ◽  
Chang Jun Li ◽  
Yong Cao ◽  
Lin Feng Wang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Rotator Cuff ◽  
Single Cell ◽  
Mechanical Stimulation ◽  
Essential Role ◽  
Primary Cilia ◽  
Underlying Mechanism ◽  
Rna Sequence ◽  
Injury Repair ◽  
Tgf Β1

Proper mechanical stimulation can improve rotator cuff enthsis injury repair. However, the underlying mechanism of mechanical stimulation promoting injury repair is still unknown. In this study, we found that Prx1+ cell was essential for murine rotator cuff enthesis development identified by single-cell RNA sequence and involved in the injury repair. Proper mechanical stimulation could promote the migration of Prx1+ cells to enhance enthesis injury repair. Meantime, TGF-β signaling and primary cilia played an essential role in mediating mechanical stimulation signaling transmission. Proper mechanical stimulation enhanced the release of active TGF-β1 to promote migration of Prx1+ cells. Inhibition of TGF-β signaling eliminated the stimulatory effect of mechanical stimulation on Prx1+ cell migration and enthesis injury repair. In addition, knockdown of Pallidin to inhibit TGF-βR2 translocation to the primary cilia or deletion of IFT88 in Prx1+ cells also restrained the mechanics-induced Prx1+ cells migration. These findings suggested that mechanical stimulation could increase the release of active TGF-β1 and enhance the mobilization of Prx1+ cells to promote enthesis injury repair via ciliary TGF-β signaling.

scholarly journals NudCL2 regulates cell migration by stabilizing both myosin-9 and LIS1 with Hsp90

2020 ◽  
Vol 11 (7) ◽  
Author(s):  
Wenwen Chen ◽  
Wei Wang ◽  
Xiaoxia Sun ◽  
Shanshan Xie ◽  
Xiaoyang Xu ◽  
...  
Keyword(s):  
Cell Migration ◽  
Single Cell ◽  
Mammalian Cells ◽  
Ectopic Expression ◽  
Heat Shock Protein 90 ◽  
Underlying Mechanism ◽  
Collective Cell Migration ◽  
Interacting Protein ◽  
Protein Levels ◽  
Protein Instability

Abstract Cell migration plays pivotal roles in many biological processes; however, its underlying mechanism remains unclear. Here, we find that NudC-like protein 2 (NudCL2), a cochaperone of heat shock protein 90 (Hsp90), modulates cell migration by stabilizing both myosin-9 and lissencephaly protein 1 (LIS1). Either knockdown or knockout of NudCL2 significantly increases single-cell migration, but has no significant effect on collective cell migration. Immunoprecipitation–mass spectrometry and western blotting analyses reveal that NudCL2 binds to myosin-9 in mammalian cells. Depletion of NudCL2 not only decreases myosin-9 protein levels, but also results in actin disorganization. Ectopic expression of myosin-9 efficiently reverses defects in actin disorganization and single-cell migration in cells depleted of NudCL2. Interestingly, knockdown of myosin-9 increases both single and collective cell migration. Depletion of LIS1, a NudCL2 client protein, suppresses both single and collective cell migration, which exhibits the opposite effect compared with myosin-9 depletion. Co-depletion of myosin-9 and LIS1 promotes single-cell migration, resembling the phenotype caused by NudCL2 depletion. Furthermore, inhibition of Hsp90 ATPase activity also reduces the Hsp90-interacting protein myosin-9 stability and increases single-cell migration. Forced expression of Hsp90 efficiently reverses myosin-9 protein instability and the defects induced by NudCL2 depletion, but not vice versa. Taken together, these data suggest that NudCL2 plays an important role in the precise regulation of cell migration by stabilizing both myosin-9 and LIS1 via Hsp90 pathway.

scholarly journals Alveolar mimics with periodic strain and its effect on the cell layer formation

2020 ◽  
Vol 117 (9) ◽  
pp. 2827-2841
Author(s):  
Milad Radiom ◽  
Yong He ◽  
Juan Peng‐Wang ◽  
Armelle Baeza‐Squiban ◽  
Jean‐François Berret ◽  
...  
Keyword(s):  
Cell Layer ◽  
Layer Formation

scholarly journals Single cell migration profiling on a microenvironmentally tunable hydrogel microstructure device that enables stem cell potency evaluation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (5) ◽  
pp. 958-972
Author(s):  
Enrique Ros ◽  
Matías Encina ◽  
Fabián González ◽  
Rafael Contreras ◽  
Patricia Luz-Crawford ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Migration ◽  
Single Cell

Detailed cell migration profiling allows for accurate correlations with therapeutic functions of mesenchymal stem cells.

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