scholarly journals Notch signaling patterns neurogenic ectoderm and regulates the asymmetric division of neural progenitors in sea urchin embryos

Development ◽  
2017 ◽  
Vol 144 (19) ◽  
pp. 3602-3611 ◽  
Author(s):  
Dan O. Mellott ◽  
Jordan Thisdelle ◽  
Robert D. Burke
Keyword(s):  
Notch Signaling ◽  
Sea Urchin ◽  
Asymmetric Division ◽  
Neural Progenitors ◽  
Sea Urchin Embryos ◽  
Neurogenic Ectoderm

scholarly journals SYNTHESIS AND STORAGE OF MICROTUBULE PROTEINS BY SEA URCHIN EMBRYOS

1971 ◽  
Vol 50 (2) ◽  
pp. 516-528 ◽  
Author(s):  
Rudolf A. Raff ◽  
Gerald Greenhouse ◽  
Kenneth W. Gross ◽  
Paul R. Gross
Keyword(s):  
Amino Acids ◽  
Sea Urchin ◽  
Messenger Rna ◽  
Binding Activity ◽  
Total Soluble Protein ◽  
Cell Stage ◽  
Lytechinus Pictus ◽  
Sea Urchin Embryos ◽  
Tritiated Amino Acids ◽  
Vinblastine Sulfate

Studies employing colchicine binding, precipitation with vinblastine sulfate, and acrylamide gel electrophoresis confirm earlier proposals that Arbacia punctulata and Lytechinus pictus eggs and embryos contain a store of microtubule proteins. Treatment of 150,000 g supernatants from sea urchin homogenates with vinblastine sulfate precipitates about 5% of the total soluble protein, and 75% of the colchicine-binding activity. Electrophoretic examination of the precipitate reveals two very prominent bands. These have migration rates identical to those of the A and B microtubule proteins of cilia. These proteins can be made radioactive at the 16 cell stage and at hatching by pulse labeling with tritiated amino acids. By labeling for 1 hr with leucine-3H in early cleavage, then culturing embryos in the presence of unlabeled leucine, removal of newly synthesized microtubule proteins from the soluble pool can be demonstrated. Incorporation of labeled amino acids into microtubule proteins is not affected by culturing embryos continuously in 20 µg/ml of actinomycin D. Microtubule proteins appear, therefore, to be synthesized on "maternal" messenger RNA. This provides the first protein encoded by stored or "masked" mRNA in sea urchin embryos to be identified.

scholarly journals Influence of dispersant application on the toxicity to sea urchin embryos of crude and bunker oils representative of prospective oil spill threats in Arctic and Sub-Arctic seas

2021 ◽  
Vol 172 ◽  
pp. 112922
Author(s):  
Laura DeMiguel-Jiménez ◽  
Nestor Etxebarria ◽  
Xabier Lekube ◽  
Urtzi Izagirre ◽  
Ionan Marigómez
Keyword(s):  
Sea Urchin ◽  
Oil Spill ◽  
Arctic Seas ◽  
Sea Urchin Embryos

scholarly journals Vegetalization Induced by Procaine and Tetracaine in Sea Urchin Embryos. (sea urchin/development/vegetalization/procaine/tetracaine)

1990 ◽  
Vol 32 (5) ◽  
pp. 489-495 ◽  
Author(s):  
Akiko Fujiwara ◽  
Kenji Kataoka ◽  
Kaori Mikami-Takei ◽  
Eigoro Tazawa ◽  
Ikuo Yasumasu
Keyword(s):  
Sea Urchin ◽  
Sea Urchin Embryos

A micronucleus assay in sea urchin embryos

1999 ◽  
Vol 446 (1) ◽  
pp. 121-127 ◽  
Author(s):  
Kyoko Saotome ◽  
Toshio Sofuni ◽  
Makoto Hayashi
Keyword(s):  
Sea Urchin ◽  
Micronucleus Assay ◽  
Sea Urchin Embryos
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