scholarly journals Anteroposterior polarity and elongation in the absence of extra-embryonic tissues and of spatially localised signalling in gastruloids: mammalian embryonic organoids

Development ◽  
2017 ◽  
Vol 144 (21) ◽  
pp. 3894-3906 ◽  
Author(s):  
David A. Turner ◽  
Mehmet Girgin ◽  
Luz Alonso-Crisostomo ◽  
Vikas Trivedi ◽  
Peter Baillie-Johnson ◽  
...  
2005 ◽  
Vol 1723 (1-3) ◽  
pp. 106-113 ◽  
Author(s):  
Hiroshi Shinohara ◽  
Tsukasa Iwasaki ◽  
Yukiko Miyazaki ◽  
Kanako Matsuo ◽  
Takayoshi Aoki ◽  
...  
Keyword(s):  

Glycobiology ◽  
1994 ◽  
Vol 4 (3) ◽  
pp. 297-305 ◽  
Author(s):  
Philippe Marschal ◽  
Virginetta Cannon ◽  
Samuel H. Barondes ◽  
Douglas N.W. Cooper

In a previous communication (Strangeways and Fell, 1926) it was shown that if the undifferentiated limb-bud of the embryonic Fowl was cultivated in vitro , it underwent a considerable amount of progressive development. This capacity for independent development in vitro possessed by an isolated organ has been further investigated, and for these later experiments the writers have employed the early embryonic eye, a structure endowed with more complex potentialities than the limb-bud. As a result of these experiments it was found that the eyes of young Fowl embryos possess, in a remarkable degree, the faculty for self-differentiation in vitro and for “organotypic” growth as defined by Maximow (1925). The previous work on organotypic growth in vitro has already been briefly outlined in the writers’ earlier paper and need not be discussed here. The expenses connected with the experiments described in this communication were met by the Medical Research Council, to whom the writers desire to express their thanks.


2010 ◽  
Vol 89 (10) ◽  
pp. 1198-1207 ◽  
Author(s):  
Sivan Cohen ◽  
Dalit Tchorsh-Yutsis ◽  
Anna Aronovich ◽  
Orna Tal ◽  
Smadar Eventov-Friedman ◽  
...  

Development ◽  
2009 ◽  
Vol 136 (15) ◽  
pp. 2613-2621 ◽  
Author(s):  
M. Peeters ◽  
K. Ottersbach ◽  
K. Bollerot ◽  
C. Orelio ◽  
M. de Bruijn ◽  
...  

2011 ◽  
Vol 356 (1) ◽  
pp. 211
Author(s):  
Avi Leibovich ◽  
Danny Ben-Zvi ◽  
Naama Barkai ◽  
Abraham Fainsod
Keyword(s):  

Development ◽  
1991 ◽  
Vol 111 (2) ◽  
pp. 337-356
Author(s):  
X.B. Shi ◽  
Z.I. Qiu ◽  
W. He ◽  
J. Frankel

Stylonychia mytilus is a dorsoventrally flattened ciliate with compound ciliary structures arranged in a specific manner on the cell surface. In mirror-image (MI) doublets of this ciliate, two nearly complete sets of ciliary structures are arrayed side-by-side, one in a normal or ‘right-handed’ (RH) arrangement, the other in a reversed or ‘left-handed’ (LH) arrangement. MI-doublets exist in two forms, one with the RH component on the right, the LH component on the left, and feeding structures near the center (‘buccal-adjoining MI-doublet’); the other with the RH component on the left, the LH component on the right, and feeding structures on the lateral edges (‘buccal-opposing MI-doublet’). We describe an operation that can generate either type of MI-doublet. This operation interchanges large anterior and posterior regions of the cell, transposing the original posterior region anteriorly (P—A) and the original anterior region posteriorly (A—P), while retaining the original anteroposterior polarity of each region. Two sets of new ciliary structures then are formed in mirror-image arrangement, with the set in the P—A region oriented normally and the set in the A—P region undergoing a reversal of polarity along its anteroposterior axis. This sometimes creates end-to-end MI forms, but more commonly produces side-by-side MI-doublets through a folding together of the P—A and A—P regions. This folding occurs because one lateral edge of the cell had been removed during the operation; if the left edge was removed, the complex folds to the left and forms a buccal-adjoining MI-doublet, whereas if the right edge was removed, the complex folds to the right and forms a buccal-opposing MI-doublet. Both types can reorganize and later divide true-to-type, although the ‘buccal-opposing’ type is by far the more stable of the two. The generation of mirror-image forms is dependent on the prior abnormal juxtaposition of regions from opposite ends of the cell, and involves a coordinated respecification of large-scale organization. We interpret this response to be a consequence of intercalation of missing intervening positional values in the zone of posterior-anterior abutment.


1953 ◽  
Vol 30 (4) ◽  
pp. 492-501 ◽  
Author(s):  
HUGH CLARK

1. Post-deposition growth of the black snake embryo is characterized by k values as follows: days 1-11, 0.46; days 11-34, 0.057; days 34-67, 0.039. 2. Total excreted nitrogen is 12.55 mg. occurring successively in development as ammonia, urea and uric acid; k value of total nitrogen production is 0.062, days 11-67, which in comparison with those of growth during this period suggest that stored protein is an energy source during a large part of development. 3. Urea is excreted into the albumen which is the principal storage reservoir and into the yolk; that which is excreted into the yolk is reabsorbed after the 45th day and re-deposited in the albumen as urea, and is in part (22%) converted to uric acid. 4. Concentration of urea in the yolk and yolk-sac continues to increase at the same rate after the 11th day (k = 0.024), although the actual amount declines after the 45th day; concentration in the embryonic tissues increases to the 58th day, then decreases sharply to hatching time; concentration in the albumen increases throughout development, reaching a concentration of approximately 500 mg.%. 5. Uric acid synthesis is believed to be preceded by urea formation, and the presence of urease in the foetal liver and kidney suggest that the urea is hydrolysed to ammonia which is then incorporated into uric acid. Site of the transformation is uncertain, though the early and persistent localization of uric acid in the chorio-allantoic membrane points to this as the organ of synthesis. Decrease in total urea content is quantitatively identical with increase in uric acid. 6. The significance of these findings in relation to development of the vertebrate cleidoic egg is discussed.


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