myoblasts incompetent encodes a zinc finger transcription factor required to specify fusion-competent myoblasts in Drosophila

Development ◽  
2002 ◽  
Vol 129 (1) ◽  
pp. 133-141 ◽  
Author(s):  
Mar Ruiz-Gómez ◽  
Nikola Coutts ◽  
Maximiliano L. Suster ◽  
Matthias Landgraf ◽  
Michael Bate
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Myoblast Fusion ◽  
Loss Of Function ◽  
Cubitus Interruptus ◽  
Zinc Finger Transcription Factor ◽  
General Pathway ◽  
Twist Expression ◽  
Fusion Competent Myoblasts ◽  
Normal Differentiation

We report a new gene, myoblasts incompetent, essential for normal myogenesis and myoblast fusion in Drosophila. myoblasts incompetent encodes a putative zinc finger transcription factor related to vertebrate Gli proteins and to Drosophila Cubitus interruptus. myoblasts incompetent is expressed in immature somatic and visceral myoblasts. Expression is predominantly in fusion-competent myoblasts and a loss-of-function mutation in myoblasts incompetent leads to a failure in the normal differentiation of these cells and a complete lack of myoblast fusion. In the mutant embryos, founder myoblasts differentiate normally and form mononucleate muscles, but genes that are specifically expressed in fusion-competent cells are not activated and the normal downregulation of twist expression in these cells fails to occur. In addition, fusion-competent myoblasts fail to express proteins characteristic of the general pathway of myogenesis such as myosin and Dmef2. Thus myoblasts incompetent appears to function specifically in the general pathway of myogenesis to control the differentiation of fusion-competent myoblasts.

scholarly journals Identification of ZBP-89 as a Novel GATA-1-Associated Transcription Factor Involved in Megakaryocytic and Erythroid Development

2008 ◽  
Vol 28 (8) ◽  
pp. 2675-2689 ◽  
Author(s):  
Andrew J. Woo ◽  
Tyler B. Moran ◽  
Yocheved L. Schindler ◽  
Seong-Kyu Choe ◽  
Nathaniel B. Langer ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Regulatory Elements ◽  
Loss Of Function ◽  
Zinc Finger Transcription Factor ◽  
Novel Proteins ◽  
Proteomic Approach ◽  
Primitive Erythropoiesis ◽  
Definitive Erythropoiesis

ABSTRACT A complete understanding of the transcriptional regulation of developmental lineages requires that all relevant factors be identified. Here, we have taken a proteomic approach to identify novel proteins associated with GATA-1, a lineage-restricted zinc finger transcription factor required for terminal erythroid and megakaryocytic maturation. We identify the Krüppel-type zinc finger transcription factor ZBP-89 as being a component of multiprotein complexes involving GATA-1 and its essential cofactor Friend of GATA-1 (FOG-1). Using chromatin immunoprecipitation assays, we show that GATA-1 and ZBP-89 cooccupy cis-regulatory elements of certain erythroid and megakaryocyte-specific genes, including an enhancer of the GATA-1 gene itself. Loss-of-function studies in zebrafish and mice demonstrate an in vivo requirement for ZBP-89 in megakaryopoiesis and definitive erythropoiesis but not primitive erythropoiesis, phenocopying aspects of FOG-1- and GATA-1-deficient animals. These findings identify ZBP-89 as being a novel transcription factor involved in erythroid and megakaryocytic development and suggest that it serves a cooperative function with GATA-1 and/or FOG-1 in a developmental stage-specific manner.

scholarly journals The apple C2H2-type zinc finger transcription factor MdZAT10 positively regulates JA-induced leaf senescence by interacting with MdBT2

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Kuo Yang ◽  
Jian-Ping An ◽  
Chong-Yang Li ◽  
Xue-Na Shen ◽  
Ya-Jing Liu ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Transcription Factor ◽  
Liquid Chromatography ◽  
Zinc Finger ◽  
Leaf Senescence ◽  
Transcriptional Activity ◽  
Molecular Mechanisms ◽  
Liquid Chromatography Mass Spectrometry ◽  
Zinc Finger Transcription Factor ◽  
Insight Into

AbstractJasmonic acid (JA) plays an important role in regulating leaf senescence. However, the molecular mechanisms of leaf senescence in apple (Malus domestica) remain elusive. In this study, we found that MdZAT10, a C2H2-type zinc finger transcription factor (TF) in apple, markedly accelerates leaf senescence and increases the expression of senescence-related genes. To explore how MdZAT10 promotes leaf senescence, we carried out liquid chromatography/mass spectrometry screening. We found that MdABI5 physically interacts with MdZAT10. MdABI5, an important positive regulator of leaf senescence, significantly accelerated leaf senescence in apple. MdZAT10 was found to enhance the transcriptional activity of MdABI5 for MdNYC1 and MdNYE1, thus accelerating leaf senescence. In addition, we found that MdZAT10 expression was induced by methyl jasmonate (MeJA), which accelerated JA-induced leaf senescence. We also found that the JA-responsive protein MdBT2 directly interacts with MdZAT10 and reduces its protein stability through ubiquitination and degradation, thereby delaying MdZAT10-mediated leaf senescence. Taken together, our results provide new insight into the mechanisms by which MdZAT10 positively regulates JA-induced leaf senescence in apple.

scholarly journals A transcript profiling approach reveals the zinc finger transcription factor ZNF191 is a pleiotropic factor

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 241 ◽  
Author(s):  
Jianzhong Li ◽  
Xia Chen ◽  
Xuelian Gong ◽  
Ying Liu ◽  
Hao Feng ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Transcript Profiling ◽  
Zinc Finger Transcription Factor

Weak Organic Acid Stress Triggers Hyperphosphorylation of the Yeast Zinc-Finger Transcription Factor War1 and Dampens Stress Adaptation

2010 ◽  
Vol 14 (5) ◽  
pp. 575-586 ◽  
Author(s):  
Ingrid E. Frohner ◽  
Christa Gregori ◽  
Dorothea Anrather ◽  
Elisabeth Roitinger ◽  
Christoph Schüller ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Organic Acid ◽  
Zinc Finger ◽  
Acid Stress ◽  
Stress Adaptation ◽  
Zinc Finger Transcription Factor ◽  
Weak Organic Acid

scholarly journals Integrated STAT3 and Ikaros Zinc Finger Transcription Factor Activities Regulate Bcl-6 Expression in CD4+ Th Cells

2017 ◽  
Vol 199 (7) ◽  
pp. 2377-2387 ◽  
Author(s):  
Kaitlin A. Read ◽  
Michael D. Powell ◽  
Chandra E. Baker ◽  
Bharath K. Sreekumar ◽  
Veronica M. Ringel-Scaia ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Zinc Finger ◽  
Th Cells ◽  
Zinc Finger Transcription Factor

scholarly journals Overexpression of Zinc Finger Transcription Factor ZAT6 Enhances Salt Tolerance

2018 ◽  
Vol 13 (1) ◽  
pp. 431-445 ◽  
Author(s):  
Wei Tang ◽  
Caroline Luo
Keyword(s):  
Transcription Factor ◽  
Salt Stress ◽  
Protein Kinase ◽  
Stress Tolerance ◽  
Zinc Finger ◽  
Salt Stress Tolerance ◽  
Dependent Protein Kinase ◽  
Zinc Finger Transcription Factor ◽  
Transgenic Cell ◽  
Dependent Protein

AbstractThe purpose of the present investigation is to examine the function of the C2H2-type zinc finger transcription factor of Arabidopsis thaliana 6 (ZAT6) in salt stress tolerance in cells of rice (Oryza sativa L.), cotton (Gossypium hirsutum L.) and slash pine (Pinus elliottii Engelm.). Cells of O. sativa, G. hirsutum, and P. elliottii overexpressing ZAT6 were generated using Agrobacterium-mediated genetic transformation. Molecular and functional analysis of transgenic cell lines demonstrate that overexpression of ZAT6 increased tolerance to salt stress by decreasing lipid peroxidation and increasing the content of abscisic acid (ABA) and GA8, as well as enhancing the activities of antioxidant enzymes such as ascorbate peroxidise (APOX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD). In rice cells, ZAT6 also increased expression of Ca2+-dependent protein kinase genes OsCPK9 and OsCPK25 by 5–7 fold under NaCl stress. Altogether, our results suggest that overexpression of ZAT6 enhanced salt stress tolerance by increasing antioxidant enzyme activity, hormone content and expression of Ca2+-dependent protein kinase in transgenic cell lines of different plant species.

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