The bHLH transcription factor hand2 plays parallel roles in zebrafish heart and pectoral fin development

Development ◽  
2000 ◽  
Vol 127 (12) ◽  
pp. 2573-2582 ◽  
Author(s):  
D. Yelon ◽  
B. Ticho ◽  
M.E. Halpern ◽  
I. Ruvinsky ◽  
R.K. Ho ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Early Stage ◽  
Bhlh Transcription Factor ◽  
Lateral Plate Mesoderm ◽  
Pectoral Fin ◽  
Lateral Plate ◽  
Anteroposterior Patterning ◽  
Cellular Processes ◽  
Myocardial Development ◽  
Tbx5 Expression

The precursors of several organs reside within the lateral plate mesoderm of vertebrate embryos. Here, we demonstrate that the zebrafish hands off locus is essential for the development of two structures derived from the lateral plate mesoderm - the heart and the pectoral fin. hands off mutant embryos have defects in myocardial development from an early stage: they produce a reduced number of myocardial precursors, and the myocardial tissue that does form is improperly patterned and fails to maintain tbx5 expression. A similar array of defects is observed in the differentiation of the pectoral fin mesenchyme: small fin buds form in a delayed fashion, anteroposterior patterning of the fin mesenchyme is absent and tbx5 expression is poorly maintained. Defects in these mesodermal structures are preceded by the aberrant morphogenesis of both the cardiogenic and forelimb-forming regions of the lateral plate mesoderm. Molecular analysis of two hands off alleles indicates that the hands off locus encodes the bHLH transcription factor Hand2, which is expressed in the lateral plate mesoderm starting at the completion of gastrulation. Thus, these studies reveal early functions for Hand2 in several cellular processes and highlight a genetic parallel between heart and forelimb development.

The forkhead transcription factor Foxf1 is required for differentiation of extra-embryonic and lateral plate mesoderm

Development ◽  
2001 ◽  
Vol 128 (2) ◽  
pp. 155-166 ◽  
Author(s):  
M. Mahlapuu ◽  
M. Ormestad ◽  
S. Enerback ◽  
P. Carlsson
Keyword(s):  
Transcription Factor ◽  
Cell Adhesion ◽  
Posterior Part ◽  
Homeobox Gene ◽  
Primitive Streak ◽  
Yolk Sac ◽  
Lateral Plate Mesoderm ◽  
Forkhead Transcription Factor ◽  
Lateral Plate ◽  
Adhesion Properties

The murine Foxf1 gene encodes a forkhead transcription factor expressed in extra-embryonic and lateral plate mesoderm and later in splanchnic mesenchyme surrounding the gut and its derivatives. We have disrupted Foxf1 and show that mutant embryos die at midgestation due to defects in mesodermal differentiation and cell adhesion. The embryos do not turn and become deformed by the constraints of a small, inflexible amnion. Extra-embryonic structures exhibit a number of differentiation defects: no vasculogenesis occurs in yolk sac or allantois; chorioallantoic fusion fails; the amnion does not expand with the growth of the embryo, but misexpresses vascular and hematopoietic markers. Separation of the bulk of yolk sac mesoderm from the endodermal layer and adherence between mesoderm of yolk sac and amnion, indicate altered cell adhesion properties and enhanced intramesodermal cohesion. A possible cause of this is misexpression of the cell-adhesion protein VCAM1 in Foxf1-deficient extra-embryonic mesoderm, which leads to co-expression of VCAM with its receptor, alpha(4)-integrin. The expression level of Bmp4 is decreased in the posterior part of the embryo proper. Consistent with this, mesodermal proliferation in the primitive streak is reduced and somite formation is retarded. Expression of Foxf1 and the homeobox gene Irx3 defines the splanchnic and somatic mesodermal layers, respectively. In Foxf1-deficient embryos incomplete separation of splanchnic and somatic mesoderm is accompanied by misexpression of Irx3 in the splanchnopleure, which implicates Foxf1 as a repressor of Irx3 and as a factor involved in coelom formation.

scholarly journals Cre/lox-controlled spatio-temporal perturbation of FGF signaling in zebrafish

2018 ◽  
Author(s):  
Lucia Kirchgeorg ◽  
Anastasia Felker ◽  
Elena Chiavacci ◽  
Christian Mosimann
Keyword(s):  
Cell Fate ◽  
Dominant Negative ◽  
Specific Cell ◽  
Lateral Plate Mesoderm ◽  
Fgf Signaling ◽  
Pectoral Fin ◽  
Lateral Plate ◽  
Developmental Processes ◽  
Spatio Temporal ◽  
Temporal Perturbation

Fibroblast Growth Factor (FGF) signaling guides multiple developmental processes including body axis formation and specific cell fate patterning. In zebrafish, genetic mutants and chemical perturbations affecting FGF signaling have uncovered key developmental processes; however, these approaches cause embryo-wide FGF signaling perturbations, rendering assessment of cell-autonomous versus non-autonomous requirements for FGF signaling in individual processes difficult. Here, we created the novel transgenic line fgfr1-dn-cargo, encoding dominant-negative Fgfr1 with fluorescent tag under combined Cre/lox and heatshock control to provide spatio-temporal perturbation of FGF signaling. Validating efficient perturbation of FGF signaling by fgfr1-dn-cargo primed with ubiquitous CreERT2, we established that primed, heatshock-induced fgfr1-dn-cargo behaves akin to pulsed treatment with the FGFR inhibitor SU5402. Priming fgfr1-dn-cargo with CreERT2 in the lateral plate mesoderm, we observed selective cardiac and pectoral fin phenotypes without drastic impact on overall embryo patterning. Harnessing lateral plate mesoderm-specific FGF inhibition, we recapitulated the cell-autonomous and temporal requirement for FGF signaling in pectoral fin outgrow, as previously hypothesized from pan-embryonic FGF inhibition. Altogether, our results establish fgfr1-dn-cargo as a genetic tool to define the spatio-temporal requirements for FGF signaling in zebrafish.

scholarly journals fibin, a novel secreted lateral plate mesoderm signal, is essential for pectoral fin bud initiation in zebrafish

2007 ◽  
Vol 303 (2) ◽  
pp. 527-535 ◽  
Author(s):  
Takashi Wakahara ◽  
Naoki Kusu ◽  
Hajime Yamauchi ◽  
Ikuo Kimura ◽  
Morichika Konishi ◽  
...  
Keyword(s):  
Lateral Plate Mesoderm ◽  
Pectoral Fin ◽  
Lateral Plate ◽  
Bud Initiation

scholarly journals Conserved and non-conserved functions of the rice homologs of the Arabidopsis trichome initiation-regulating MBW complex proteins

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Kaijie Zheng ◽  
Xutong Wang ◽  
Yating Wang ◽  
Shucai Wang
Keyword(s):  
Transcription Factor ◽  
Anthocyanin Biosynthesis ◽  
Transcriptional Activator ◽  
Myb Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Seed Color ◽  
Transcription Activator ◽  
Root Hair Formation ◽  
Protoplast Transfection ◽  
Mbw Complex

Abstract Background Trichome initiation in Arabidopsis is regulated by a MYB-bHLH-WD40 (MBW) transcriptional activator complex formed by the R2R3 MYB transcription factor GLABRA1 (GL1), MYB23 or MYB82, the bHLH transcription factor GLABRA3 (GL3), ENHANCER OF GLABRA3 (EGL3) or TRANSPARENT TESTA8 (TT8), and the WD40-repeat protein TRANSPARENT TESTA GLABRA1 (TTG1). However, the functions of the rice homologs of the MBW complex proteins remained uncharacterized. Results Based on amino acid sequence identity and similarity, and protein interaction prediction, we identified OsGL1s, OsGL3s and OsTTG1s as rice homologs of the MBW complex proteins. By using protoplast transfection, we show that OsGL1D, OsGL1E, OsGL3B and OsTTG1A were predominantly localized in the nucleus, OsGL3B functions as a transcriptional activator and is able to interact with GL1 and TTG1. By using yeast two-hybrid and protoplast transfection assays, we show that OsGL3B is able to interact with OsGL1E and OsTTG1A, and OsGL1E and OsTTG1A are also able to interact with GL3. On the other hand, we found that OsGL1D functions as a transcription activator, and it can interact with GL3 but not OsGL3B. Furthermore, our results show that expression of OsTTG1A in the ttg1 mutant restored the phenotypes including alternations in trichome and root hair formation, seed color, mucilage production and anthocyanin biosynthesis, indicating that OsTTG1A and TTG1 may have similar functions. Conclusion These results suggest that the rice homologs of the Arabidopsis MBW complex proteins are able to form MBW complexes, but may have conserved and non-conserved functions.

scholarly journals An Atypical bHLH Transcription Factor Regulates Early Xylem Development Downstream of Auxin

2013 ◽  
Vol 54 (3) ◽  
pp. 398-405 ◽  
Author(s):  
Kyoko Ohashi-Ito ◽  
Manami Matsukawa ◽  
Hiroo Fukuda
Keyword(s):  
Transcription Factor ◽  
Bhlh Transcription Factor ◽  
Xylem Development
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