Dpp receptor levels contribute to shaping the Dpp morphogen gradient in the Drosophila wing imaginal disc

Development ◽  
1998 ◽  
Vol 125 (24) ◽  
pp. 4901-4907 ◽  
Author(s):  
T. Lecuit ◽  
S.M. Cohen
Keyword(s):  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Morphogen Gradient ◽  
Axis Formation ◽  
Present Evidence ◽  
Drosophila Wing ◽  
Spatial Domains ◽  
Low Levels ◽  
Wing Pouch ◽  
Anterior Posterior

Axis formation in the Drosophila wing depends on the localized expression of the secreted signaling molecule Decapentaplegic (Dpp). Dpp acts directly at a distance to specify discrete spatial domains, suggesting that it functions as a morphogen. Expression levels of the Dpp receptor thick veins (tkv) are not uniform along the anterior-posterior axis of the wing imaginal disc. Receptor levels are low where Dpp induces its targets Spalt and Omb in the wing pouch. Receptor levels increase in cells farther from the source of Dpp in the lateral regions of the disc. We present evidence that Dpp signaling negatively regulates tkv expression and that the level of receptor influences the effective range of the Dpp gradient. High levels of tkv sensitize cells to low levels of Dpp and also appear to limit the movement of Dpp outside the wing pouch. Thus receptor levels help to shape the Dpp gradient.

Boundaries in the Drosophila wing imaginal disc organize vein-specific genetic programs

Development ◽  
1998 ◽  
Vol 125 (21) ◽  
pp. 4245-4257 ◽  
Author(s):  
B. Biehs ◽  
M.A. Sturtevant ◽  
E. Bier
Keyword(s):  
Gene Expression ◽  
Cell Fate ◽  
Short Range ◽  
Imaginal Disc ◽  
Larval Instar ◽  
Wing Imaginal Disc ◽  
Control Of Gene Expression ◽  
Present Evidence ◽  
The Third ◽  
Drosophila Wing

Previous studies have suggested that vein primordia in Drosophila form at boundaries along the A/P axis between discrete sectors of the larval wing imaginal disc. Genes involved in initiating vein development during the third larval instar are expressed either in narrow stripes corresponding to vein primordia or in broader ‘provein’ domains consisting of cells competent to become veins. In addition, genes specifying the alternative intervein cell fate are expressed in complementary intervein regions. The regulatory relationships between genes expressed in narrow vein primordia, in broad provein stripes and in interveins remains unknown, however. In this manuscript, we provide additional evidence for veins forming in narrow stripes at borders of A/P sectors. These experiments further suggest that narrow vein primordia produce secondary short-range signal(s), which activate expression of provein genes in a broad pattern in neighboring cells. We also show that crossregulatory interactions among genes expressed in veins, proveins and interveins contribute to establishing the vein-versus-intervein pattern, and that control of gene expression in vein and intervein regions must be considered on a stripe-by-stripe basis. Finally, we present evidence for a second set of vein-inducing boundaries lying between veins, which we refer to as paravein boundaries. We propose that veins develop at both vein and paravein boundaries in more ‘primitive’ insects, which have up to twice the number of veins present in Drosophila. We present a model in which different A/P boundaries organize vein-specific genetic programs to govern the development of individual veins.

scholarly journals Compartments and the control of growth in the Drosophila wing imaginal disc

Development ◽  
2006 ◽  
Vol 133 (22) ◽  
pp. 4421-4426 ◽  
Author(s):  
F. A. Martin ◽  
G. Morata
Keyword(s):  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

Role of knot (kn) in Wing Patterning in Drosophila

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1203-1212 ◽  
Author(s):  
Katerina Nestoras ◽  
Helena Lee ◽  
Jym Mohler
Keyword(s):  
Hedgehog Signaling ◽  
Imaginal Disc ◽  
Hedgehog Signaling Pathway ◽  
Posterior Compartment ◽  
Drosophila Wing ◽  
Compartment Boundary ◽  
Hh Signaling ◽  
Embryonic Segmentation ◽  
Anterior Posterior

We have undertaken a genetic analysis of new strong alleles of knot (kn). The original kn1 mutation causes an alteration of wing patterning similar to that associated with mutations of fused (fu), an apparent fusion of veins 3 and 4 in the wing. However, unlike fu, strong kn mutations do not affect embryonic segmentation and indicate that kn is not a component of a general Hh (Hedgehog)-signaling pathway. Instead we find that kn has a specific role in those cells of the wing imaginal disc that are subject to ptc-mediated Hh-signaling. Our results suggest a model for patterning the medial portion of the Drosophila wing, whereby the separation of veins 3 and 4 is maintained by kn activation in the intervening region in response to Hh-signaling across the adjacent anterior-posterior compartment boundary.

slimb coordinates wg and dpp expression in the dorsal-ventral and anterior-posterior axes during limb development

Development ◽  
1998 ◽  
Vol 125 (17) ◽  
pp. 3411-3416 ◽  
Author(s):  
N.A. Theodosiou ◽  
S. Zhang ◽  
W.Y. Wang ◽  
T. Xu
Keyword(s):  
Genetic Analysis ◽  
Limb Development ◽  
Imaginal Disc ◽  
Essential Role ◽  
Present Evidence ◽  
First Time ◽  
Anterior Posterior

In the Drosophila leg disc, wingless (wg) and decapentaplegic (dpp) are expressed in a ventral-anterior and dorsal-anterior stripe of cells, respectively. This pattern of expression is essential for proper limb development. While the Hedgehog (Hh) pathway regulates dpp and wg expression in the anterior-posterior (A/P) axis, mechanisms specifying their expression in the dorsal-ventral (D/V) axis are not well understood. We present evidence that slimb mutant clones in the disc deregulate wg and dpp expression in the D/V axis. This suggests for the first time that their expression in the D/V axis is actively regulated during imaginal disc development. Furthermore, slimb is unique in that it also deregulates wg and dpp in the A/P axis. The misexpression phenotypes of slimb- clones indicate that the regulation of wg and dpp expression is coordinated in both axes, and that slimb plays an essential role in integrating A/P and D/V signals for proper patterning during development. Our genetic analysis further reveals that slimb intersects the A/P pathway upstream of smoothened (smo).

Control of growth and patterning of the Drosophila wing imaginal disc by EGFR-mediated signaling

Development ◽  
2002 ◽  
Vol 129 (6) ◽  
pp. 1369-1376 ◽  
Author(s):  
Myriam Zecca ◽  
Gary Struhl
Keyword(s):  
Gene Expression ◽  
Imaginal Disc ◽  
Ectopic Expression ◽  
Growth Factor Receptor ◽  
Wing Disc ◽  
Wing Imaginal Disc ◽  
Egfr Signaling ◽  
Drosophila Wing ◽  
Compartment Boundary ◽  
Egfr Ligand

The subdivision of the Drosophila wing imaginal disc into dorsoventral (DV) compartments and limb-body wall (wing-notum) primordia depends on Epidermal Growth Factor Receptor (EGFR) signaling, which heritably activates apterous (ap) in D compartment cells and maintains Iroquois Complex (Iro-C) gene expression in prospective notum cells. We examine the source, identity and mode of action of the EGFR ligand(s) that specify these subdivisions. Of the three known ligands for the Drosophila EGFR, only Vein (Vn), but not Spitz or Gurken, is required for wing disc development. We show that Vn activity is required specifically in the dorsoproximal region of the wing disc for ap and Iro-C gene expression. However, ectopic expression of Vn in other locations does not reorganize ap or Iro-C gene expression. Hence, Vn appears to play a permissive rather than an instructive role in organizing the DV and wing-notum segregations, implying the existance of other localized factors that control where Vn-EGFR signaling is effective. After ap is heritably activated, the level of EGFR activity declines in D compartment cells as they proliferate and move ventrally, away from the source of the instructive ligand. We present evidence that this reduction is necessary for D and V compartment cells to interact along the compartment boundary to induce signals, like Wingless (Wg), which organize the subsequent growth and differentiation of the wing primordium.

scholarly journals RNAi-Mediated Knockdown Showing Impaired Cell Survival in Drosophila Wing Imaginal Disc

2009 ◽  
Vol 3 ◽  
pp. GRSB.S2100 ◽  
Author(s):  
Makoto Umemori ◽  
Okiko Habara ◽  
Tatsunori Iwata ◽  
Kousuke Maeda ◽  
Kana Nishinoue ◽  
...  
Keyword(s):  
Cell Survival ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing

Cell-level 3D reconstruction and quantification of the Drosophila wing imaginal disc

2019 ◽  
Vol 15 (2) ◽  
pp. 174
Author(s):  
Christian Dahmann ◽  
Frank Jülicher ◽  
Linge Bai ◽  
David E. Breen ◽  
Liyuan Sui
Keyword(s):  
3D Reconstruction ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Cell Level ◽  
Drosophila Wing

scholarly journals Microvilli-derived extracellular vesicles carry Hedgehog morphogenic signals for Drosophila wing imaginal disc development

2021 ◽  
Author(s):  
Ilse Hurbain ◽  
Anne-Sophie Macé ◽  
Maryse Romao ◽  
Elodie Prince ◽  
Lucie Sengmanivong ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Imaginal Disc ◽  
Wing Imaginal Disc ◽  
Drosophila Wing
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