Ommatidial polarity in the Drosophila eye is determined by the direction of furrow progression and local interactions

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4247-4256 ◽  
Author(s):  
D.I. Strutt ◽  
M. Mlodzik
Keyword(s):  
Mirror Image ◽  
Hedgehog Pathway ◽  
Ordered Structure ◽  
Local Interactions ◽  
Eye Disc ◽  
Drosophila Eye

The adult eye of Drosophila is a highly ordered structure. It is composed of about 800 ommatidia, each displaying precise polarity. The ommatidia are arranged about an axis of mirror image symmetry, the equator, which lies along the dorsoventral midline of the eye. We use hedgehog pathway mutants to induce ectopic morphogenetic furrows and use these as a tool to investigate the establishment of ommatidial polarity. Our results show that ommatidial clusters are self-organising units whose polarity in one axis is determined by the direction of furrow progression, and which can independently define the position of an equator without reference to the global coordinates of the eye disc.

frizzled regulates mirror-symmetric pattern formation in the Drosophila eye

Development ◽  
1995 ◽  
Vol 121 (9) ◽  
pp. 3045-3055 ◽  
Author(s):  
L. Zheng ◽  
J. Zhang ◽  
R.W. Carthew
Keyword(s):  
Pattern Formation ◽  
Cell Surface ◽  
Transmembrane Protein ◽  
Photoreceptor Cells ◽  
Mirror Image ◽  
Morphogenetic Furrow ◽  
Symmetric Pattern ◽  
Mosaic Analysis ◽  
Drosophila Eye ◽  
A Cell

Coordinated morphogenesis of ommatidia during Drosophila eye development establishes a mirror-image symmetric pattern across the entire eye bisected by an anteroposterior equator. We have investigated the mechanisms by which this pattern formation occurs and our results suggest that morphogenesis is coordinated by a graded signal transmitted bidirectionally from the presumptive equator to the dorsal and ventral poles. This signal is mediated by frizzled, which encodes a cell surface transmembrane protein. Mosaic analysis indicates that frizzled acts non-autonomously in an equatorial to polar direction. It also indicates that relative levels of frizzled in photoreceptor cells R3 and R4 of each ommatidium affect their positional fate choices such that the cell with greater frizzled activity becomes an R3 cell and the cell with less frizzled activity becomes an R4 cell. Moreover, this bias affects the choice an ommatidium makes as to which direction to rotate. Equator-outwards progression of elav expression and expression of the nemo gene in the morphogenetic furrow are regulated by frizzled, which itself is dynamically expressed about the morphogenetic furrow. We propose that frizzled mediates a bidirectional signal emanating from the equator.

scholarly journals STRIPAK–PP2A regulates Hippo-Yorkie signaling to suppress retinal fate in the Drosophila eye disc peripodial epithelium

2020 ◽  
Vol 133 (10) ◽  
pp. jcs237834 ◽  
Author(s):  
Scott J. Neal ◽  
Qingxiang Zhou ◽  
Francesca Pignoni
Keyword(s):  
Eye Disc ◽  
Drosophila Eye

Broad-complex, but not ecdysone receptor, is required for progression of the morphogenetic furrow in the Drosophila eye

Development ◽  
2001 ◽  
Vol 128 (1) ◽  
pp. 1-11 ◽  
Author(s):  
C.A. Brennan ◽  
T.R. Li ◽  
M. Bender ◽  
F. Hsiung ◽  
K. Moses
Keyword(s):  
Zinc Finger ◽  
Receptor Gene ◽  
Ecdysone Receptor ◽  
Morphogenetic Furrow ◽  
Response Gene ◽  
Ecdysteroid Receptor ◽  
Receptor Isoforms ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Broad Complex

The progression of the morphogenetic furrow in the developing Drosophila eye is an early metamorphic, ecdysteroid-dependent event. Although Ecdysone receptor-encoded nuclear receptor isoforms are the only known ecdysteroid receptors, we show that the Ecdysone receptor gene is not required for furrow function. DHR78, which encodes another candidate ecdysteroid receptor, is also not required. In contrast, zinc finger-containing isoforms encoded by the early ecdysone response gene Broad-complex regulate furrow progression and photoreceptor specification. br-encoded Broad-complex subfunctions are required for furrow progression and proper R8 specification, and are antagonized by other subfunctions of Broad-complex. There is a switch from Broad complex Z2 to Z1 zinc-finger isoform expression at the furrow which requires Z2 expression and responds to Hedgehog signals. These results suggest that a novel hormone transduction hierarchy involving an uncharacterized receptor operates in the eye disc.

Role of the morphogenetic furrow in establishing polarity in the Drosophila eye

Development ◽  
1995 ◽  
Vol 121 (12) ◽  
pp. 4085-4094 ◽  
Author(s):  
F. Chanut ◽  
U. Heberlein
Keyword(s):  
Ectopic Expression ◽  
Morphogenetic Furrow ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Retinal Epithelium ◽  
Anteroposterior Polarity ◽  
Crystalline Array

The Drosophila retina is a crystalline array of 800 ommatidia whose organization and assembly suggest polarization of the retinal epithelium along anteroposterior and dorsoventral axes. The retina develops by a stepwise process following the posterior-to-anterior progression of the morphogenetic furrow across the eye disc. Ectopic expression of hedgehog or local removal of patched function generates ectopic furrows that can progress in any direction across the disc leaving in their wake differentiating fields of ectopic ommatidia. We have studied the effect of these ectopic furrows on the polarity of ommatidial assembly and rotation. We find that the anteroposterior asymmetry of ommatidial assembly parallels the progression of ectopic furrows, regardless of their direction. In addition, ommatidia developing behind ectopic furrows rotate coordinately, forming equators in various regions of the disc. Interestingly, the expression of a marker normally restricted to the equator is induced in ectopic ommatidial fields. Ectopic equators are stable as they persist to adulthood, where they can coexist with the normal equator. Our results suggest that ectopic furrows can impart polarity to the disc epithelium, regarding the direction of both assembly and rotation of ommatidia. We propose that these processes are polarized as a consequence of furrow propagation, while more global determinants of dorsoventral and anteroposterior polarity may act less directly by determining the site of furrow initiation.

Lobe mediates Notch signaling to control domain-specific growth in the Drosophila eye disc

Development ◽  
2002 ◽  
Vol 129 (17) ◽  
pp. 4005-4013 ◽  
Author(s):  
Joshua J. Chern ◽  
Kwang-Wook Choi
Keyword(s):  
Mirror Symmetry ◽  
Tissue Loss ◽  
Domain Specific ◽  
Eye Growth ◽  
Proliferative Effect ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Domain Loss ◽  
Necessary And Sufficient ◽  
Novel Protein

Notch (N) activation at the dorsoventral (DV) boundary of the Drosophila eye is required for early eye primordium growth. Despite the apparent DV mirror symmetry, some mutations cause a preferential loss of the ventral domain, suggesting that the growth of individual domains is asymmetrically regulated. We show that the Lobe (L) gene is required non-autonomously for ventral growth but not dorsal growth, and that it mediates the proliferative effect of midline N signaling in a ventral-specific manner. L encodes a novel protein with a conserved domain. Loss of L suppresses the overproliferation phenotype of constitutive N activation in the ventral, but not in the dorsal eye, and gain of L rescues ventral tissue loss in N mutant background. Furthermore, L is necessary and sufficient for the ventral expression of a N ligand, Serrate (Ser), which affects ventral growth. Our data suggest that the control of ventral Ser expression by L represents a molecular mechanism that governs asymmetrical eye growth.

scholarly journals Integrins Regulate Apical Constriction via Microtubule Stabilization in the Drosophila Eye Disc Epithelium

Cell Reports ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. 2043-2055 ◽  
Author(s):  
Vilaiwan M. Fernandes ◽  
Kasandra McCormack ◽  
Lindsay Lewellyn ◽  
Esther M. Verheyen
Keyword(s):  
Apical Constriction ◽  
Microtubule Stabilization ◽  
Eye Disc ◽  
Drosophila Eye

Drosophila eye disc margin is a center for organizing long-range planar polarity

genesis ◽  
2004 ◽  
Vol 39 (1) ◽  
pp. 26-37 ◽  
Author(s):  
Janghoo Lim ◽  
Kwang-Wook Choi
Keyword(s):  
Long Range ◽  
Planar Polarity ◽  
Eye Disc ◽  
Drosophila Eye

Ommatidial development in Drosophila eye disc fragments

1986 ◽  
Vol 117 (2) ◽  
pp. 663-671 ◽  
Author(s):  
Richard M. Lebovitz ◽  
Donald F. Ready
Keyword(s):  
Eye Disc ◽  
Drosophila Eye

scholarly journals A Model of the Spatio-temporal Dynamics of Drosophila Eye Disc Development

2016 ◽  
Vol 12 (9) ◽  
pp. e1005052 ◽  
Author(s):  
Patrick Fried ◽  
Máximo Sánchez-Aragón ◽  
Daniel Aguilar-Hidalgo ◽  
Birgitta Lehtinen ◽  
Fernando Casares ◽  
...  
Keyword(s):  
Temporal Dynamics ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Spatio Temporal

Hedgehog is an indirect regulator of morphogenetic furrow progression in the Drosophila eye disc

Development ◽  
1997 ◽  
Vol 124 (17) ◽  
pp. 3233-3240 ◽  
Author(s):  
D.I. Strutt ◽  
M. Mlodzik
Keyword(s):  
Pattern Formation ◽  
Imaginal Disc ◽  
Morphogenetic Furrow ◽  
Anterior Edge ◽  
Eye Disc ◽  
Drosophila Eye ◽  
Eye Imaginal Disc ◽  
Inductive Signal ◽  
Rate Of Movement

Pattern formation in the eye imaginal disc of Drosophila occurs in a wave that moves from posterior to anterior. The anterior edge of this wave is marked by a contracted band of cells known as the morphogenetic furrow, behind which photoreceptors differentiate. The movement of the furrow is dependent upon the secretion of the signalling protein Hedgehog (Hh) by more posterior cells, and it has been suggested that Hh acts as an inductive signal to induce cells to enter a furrow fate and begin differentiation. To further define the role of Hh in this process, we have analysed clones of cells lacking the function of the smoothened (smo) gene, which is required for transduction of the Hh signal and allows the investigation of the autonomous requirement for hh signalling. These experiments demonstrate that the function of hh in furrow progression is indirect. Cells that cannot receive/transduce the Hh signal are still capable of entering a furrow fate and differentiating normally. However, hh is required to promote furrow progression and regulate its rate of movement across the disc, since the furrow is significantly delayed in smo clones.

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