The expression pattern of a novel gene encoding brain-fatty acid binding protein correlates with neuronal and glial cell development

Development ◽  
1994 ◽  
Vol 120 (9) ◽  
pp. 2637-2649 ◽  
Author(s):  
A. Kurtz ◽  
A. Zimmer ◽  
F. Schnutgen ◽  
G. Bruning ◽  
F. Spener ◽  
...  
Keyword(s):  
Nervous System ◽  
Fatty Acid ◽  
Expression Pattern ◽  
Glial Cells ◽  
Binding Proteins ◽  
Cloning And Expression ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Radial Glial Cells ◽  
Radial Glial

Fatty acid binding proteins (FABPs) are a multigene family of small intracellular proteins that bind hydrophobic ligands. In this report we describe the cloning and expression pattern of a novel member of this gene family that is specifically expressed in the developing and adult nervous system and thus was designated brain (B)-FABP. B-FABP is closely related to heart (H)-FABP with 67% amino acid identity. B-FABP expression was first detected at mouse embryonic day 10 in neuroepithelial cells and its pattern correlates with early neuronal differentiation. Upon further development, B-FABP was confined to radial glial cells and immature astrocytes. B-FABP mRNA and protein were found in glial cells of the peripheral nervous system such as satellite cells of spinal and cranial ganglia and ensheathing cells of the olfactory nerve layer from as early as embryonic day 11 until adulthood. In the adult mouse brain, B-FABP was found in the glia limitans, in radial glial cells of the hippocampal dentate gyrus and Bergman glial cells. These findings suggest a function of B-FABP during neurogenesis or neuronal migration in the developing nervous system. The partially overlapping expression pattern with that of cellular retinoid binding proteins suggests that B-FABP is involved in the metabolism of a so far unknown hydrophobic ligand with potential morphogenic activity during CNS development.

scholarly journals Expression Pattern of Fatty Acid Binding Proteins in Celiac Disease Enteropathy

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Natalia M. Bottasso Arias ◽  
Marina García ◽  
Constanza Bondar ◽  
Luciana Guzman ◽  
Agustina Redondo ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Fatty Acid ◽  
Expression Pattern ◽  
Intestinal Mucosa ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Small Intestinal Mucosa ◽  
Mrna Levels ◽  
Fatty Acid Binding ◽  
Small Intestinal

Celiac disease (CD) is an immune-mediated enteropathy that develops in genetically susceptible individuals following exposure to dietary gluten. Severe changes at the intestinal mucosa observed in untreated CD patients are linked to changes in the level and in the pattern of expression of different genes. Fully differentiated epithelial cells express two isoforms of fatty acid binding proteins (FABPs): intestinal and liver, IFABP and LFABP, respectively. These proteins bind and transport long chain fatty acids and also have other important biological roles in signaling pathways, particularly those related to PPARγand inflammatory processes. Herein, we analyze the serum levels of IFABP and characterize the expression of both FABPs at protein and mRNA level in small intestinal mucosa in severe enteropathy and normal tissue. As a result, we observed higher levels of circulating IFABP in untreated CD patients compared with controls and patients on gluten-free diet. In duodenal mucosa a differential FABPs expression pattern was observed with a reduction in mRNA levels compared to controls explained by the epithelium loss in severe enteropathy. In conclusion, we report changes in FABPs’ expression pattern in severe enteropathy. Consequently, there might be alterations in lipid metabolism and the inflammatory process in the small intestinal mucosa.

scholarly journals Fatty acid-binding proteins and fatty acid synthase influence glial reactivity and promote the formation of Müller glia-derived progenitor cells in the avian retina

2021 ◽  
Author(s):  
Warren A. Campbell ◽  
Allen Tangeman ◽  
Heithem M. El-Hodiri ◽  
Evan C. Hawthorn ◽  
Maddie Hathoot ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Progenitor Cells ◽  
Glial Cells ◽  
Fatty Acid Synthase ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Cytokine Signaling ◽  
Fatty Acid Binding ◽  
Chick Retina ◽  
Dying Cells

AbstractThe capacity for retinal regeneration varies greatly across vertebrates species. A recent comparative epigenetic and transcriptomic investigation of Müller glial (MG) in the retinas of fish, birds and mice revealed that Fatty Acid Binding Proteins (FABPs) are among the most highly up-regulated genes in activated chick MG (Hoang et al., 2020). Herein we provide an in-depth follow-up investigation to describe patterns of expression and how FABPs and fatty acid synthase (FASN) influence glial cells in the chick retina. During development, FABP7 is highly expressed by embryonic retinal progenitor cells (eRPCs) and maturing MG, whereas FABP5 is gradually up-regulated in maturing MG and remains elevated in mature glial cells. PMP2 (FABP8) is expressed by oligodendrocytes and FABP5 is expressed by non-astrocytic inner retinal glial cells, and both of these FABPs are significantly up-regulated in activated MG in damaged or growth factor-treated retinas. In addition to suppressing the formation of MGPCs, we find that FABP-inhibition suppressed the accumulation of proliferating microglia, although the microglia appeared highly reactive. scRNA-seq analyses of cells treated with FABP-inhibitor revealed distinct changes in patterns of expression suggesting that FABPs are involved in the transitions of MG from a resting state to a reactive state and conversion from reactive MG to MGPCs. Inhibition of FABPs in undamaged retinas had a significant impact upon the transcriptomic profiles of MG, with up-regulation of genes associated with gliogenesis, decreases in genes associated with neurogenesis, and suppression of the ability of MG to become MGPCs. scRNA-seq analyses of microglia indicated that FABP inhibition enhances gene modules related to reactivity, proliferation and cytokine signaling. We find that the proliferation of retinal progenitors in the circumferential marginal zone (CMZ) is unaffected by FABP-inhibitor. Upstream of FABP activity, we inhibited FASN in damaged retinas, which reduced numbers of dying cells, increased the proliferation of microglia, and potently suppressed the formation MGPCs in damaged retinas. We conclude that the activity of FASN and FABPs are required early during the formation of proliferating MGPCs. Fatty acid metabolism and cell signaling involving fatty acids are important in regulating glial homeostasis in the retina, and the dedifferentiation and proliferation of microglia and MGPCs.

Isolation, Cloning, and Expression of Fatty-Acid Binding Proteins fromFasciola gigantica

1997 ◽  
Vol 85 (1) ◽  
pp. 86-91 ◽  
Author(s):  
Peter M. Smooker ◽  
Danielle E. Hickford ◽  
Sam A. Vaiano ◽  
Terry W. Spithill
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Cloning And Expression ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding

scholarly journals Fatty acid transfer between multilamellar liposomes and fatty acid-binding proteins.

1984 ◽  
Vol 259 (21) ◽  
pp. 13395-13401 ◽  
Author(s):  
P Brecher ◽  
R Saouaf ◽  
J M Sugarman ◽  
D Eisenberg ◽  
K LaRosa
Keyword(s):  
Fatty Acid ◽  
Binding Proteins ◽  
Fatty Acid Binding Proteins ◽  
Fatty Acid Binding ◽  
Multilamellar Liposomes
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