A fate map of the epiblast of the early chick embryo

Development ◽  
1994 ◽  
Vol 120 (10) ◽  
pp. 2879-2889 ◽  
Author(s):  
Y. Hatada ◽  
C.D. Stern
Keyword(s):  
Chick Embryo ◽  
Primitive Streak ◽  
Cell Types ◽  
Cell Populations ◽  
Fate Map ◽  
Early Chick Embryo ◽  
Carbocyanine Dyes

We have used carbocyanine dyes (DiI and DiO) to generate fate maps for the epiblast layer of the chick embryo between stage X and the early primitive streak stage (stages 2–3). The overall distribution of presumptive cell types in these maps is similar to that described for other laboratory species (zebrafish, frog, mouse). Our maps also reveal certain patterns of movement for these presumptive areas. Most areas converge towards the midline and then move anteriorly along it. Interestingly, however, some presumptive tissue types do not take part in these predominant movements, but behave in a different way, even if enclosed within an area that does undergo medial convergence and anterior movement. The apparently independent behaviour of certain cell populations suggests that at least some presumptive cell types within the epiblast are already specified at preprimitive streak stages.

Fates and migratory routes of primitive streak cells in the chick embryo

Development ◽  
1996 ◽  
Vol 122 (5) ◽  
pp. 1523-1534 ◽  
Author(s):  
D. Psychoyos ◽  
C.D. Stern
Keyword(s):  
Chick Embryo ◽  
Primitive Streak ◽  
Final Position ◽  
Fate Map ◽  
Early Chick Embryo ◽  
Carbocyanine Dyes ◽  
Early Stages ◽  
Migratory Routes ◽  
Pass Through ◽  
Different Tissues

We have used carbocyanine dyes to fate map the primitive streak in the early chick embryo, from stages 3+ (mid-primitive streak) to 9 (8 somites). We show that presumptive notochord, foregut and medial somite do not originate solely from Hensen's node, but also from the anterior primitive streak. At early stages (4- and 4), there is no correlation between specific anteroposterior levels of the primitive streak and the final position of their descendants in the notochord. We describe in detail the contribution of specific levels of the primitive streak to the medial and lateral halves of the somites. To understand how the descendants of labelled cells reach their destinations in different tissues, we have followed the movement of labelled cells during their emigration from the primitive streak in living embryos, and find that cells destined to different structures follow defined pathways of movement, even if they arise from similar positions in the streak. Somite and notochord precursors migrate anteriorly within the streak and pass through different portions of the node; this provides an explanation for the segregation of notochord and somite territories in the node.

Segmental repetition of neuronal phenotype sets in the chick embryo hindbrain

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 151-162 ◽  
Author(s):  
J.D. Clarke ◽  
A. Lumsden
Keyword(s):  
Chick Embryo ◽  
Cell Types ◽  
Spatial Segregation ◽  
Differential Migration ◽  
Neuronal Phenotype ◽  
Regional Diversity ◽  
Early Chick Embryo ◽  
Data Support ◽  
Neuronal Types ◽  
Neuronal Tracers

The neurons within the segmented hindbrain of the early chick embryo have been mapped with the neuronal tracers HRP and fluorescent lysinated dextran. We have categorised neurons according to their axonal pathways and have then compared rhombomeres with respect to the number and class of neurons present. The results indicate that most rhombomeres are similar in that they contain the same set of basic neuronal types but differ in that particular neuronal types are more abundant in some rhombomeres than others. The data support the concept that the hindbrain develops according to ‘variations on a segmental theme’ rather than ‘each segment is unique’. Many of the cell types occupy distinct mediolateral domains that are probably established by both the differential migration of some neuronal classes and the spatial segregation of distinct precursors. The caudal rhombomeres 7 and 8 are exceptional in that they do not have the full set of basic neuronal types and also contain two additional medial cell types that are not present rostrally. The mechanisms that may generate the regional diversity apparent in the more mature hindbrain are discussed.

Erythroid cell differentiation in unincubated chick blastoderm in culture

Development ◽  
1980 ◽  
Vol 58 (1) ◽  
pp. 209-216
Author(s):  
Nikolas Zagris
Keyword(s):  
Primitive Streak ◽  
Cell Types ◽  
Ventral Side ◽  
Erythroid Cell ◽  
Cell Populations ◽  
Axis Formation ◽  
Yolk Mass ◽  
Chick Blastoderm ◽  
Serum Free ◽  
Erythroid Cell Differentiation

Morphologically distinct erythroid cell types characteristic of the primitive and the definitiveerythroid cell lines, and embryonic and adult haemoglobins are produced when the unin-cubated chick blastoderm is cultured ventral side down on a filter raft to inhibit morphogeneticmovements and subsequent primitive-streak formation mechanically in serum-free minimalessential medium. The primitive and definitive erythroid cell populations appear consecutivelyin culture even though there is no axis formation nor apparent morphogenesis. The informa-tion to produce both the early and late haemoglobins and erythroid cell types is independentof axis formation and of specific extra-embryonic influences, such as progressive inductionexerted by the yolk mass.

Transient embryonic antigens in the chick

Development ◽  
1964 ◽  
Vol 12 (3) ◽  
pp. 511-516
Author(s):  
D. J. McCallion ◽  
J. C. Trott
Keyword(s):  
Spinal Cord ◽  
Chick Embryo ◽  
Primitive Streak ◽  
Adult Chicken ◽  
Tissue Specific ◽  
Chicken Brain ◽  
Early Chick Embryo ◽  
Embryonic Antigens ◽  
Specific Antigens ◽  
The Brain

The Presence of an organ antigen in the early chick embryo was first demonstrated by Schechtman (1948). He found that an antigenic substance common to brain, heart, liver and muscle of chicks at hatching is already present in primitive streak and early neurula stages of the embryo. This observation, with respect to brain and heart, was subsequently confirmed by Ebert (1950). McCallion & Langman (1964) have recently demonstrated that there are at least eight antigenic substances in the adult chicken brain that are class-specific but that are more or less common to other organs, with only quantitative differences. These authors have further demonstrated that there are at least three, possibly as many as five, antigenic substances in adult chicken brain that are not only class-specific but also tissue-specific, occurring only in the brain, spinal cord, nervous retina and nerves. The non-specific antigens appear progressively during the first 4 days of incubation.

Fate mapping and cell lineage analysis of Hensen's node in the chick embryo

Development ◽  
1991 ◽  
Vol 112 (2) ◽  
pp. 615-626 ◽  
Author(s):  
M.A. Selleck ◽  
C.D. Stern
Keyword(s):  
Chick Embryo ◽  
Cell Lineage ◽  
Primitive Streak ◽  
Cell Types ◽  
Intracellular Injection ◽  
Anterior Midline ◽  
Stage 4 ◽  
Separate Region ◽  
Notochord Cells ◽  
Lineage Analysis

Fate maps of chick Hensen's node were generated using DiI and the lineage of individual cells studied by intracellular injection of lysine-rhodamine-dextran (LRD). The cell types contained within the node are organized both spatially and temporally. At the definitive primitive streak stage (Hamburger and Hamilton stage 4), Hensen's node contains presumptive notochord cells mainly in its anterior midline and presumptive somite cells in more lateral regions. Early in development it also contains presumptive endoderm cells. At all stages studied (stages 3–9), some individual cells contribute progeny to more than one of these tissues. The somitic precursors in Hensen's node only contribute to the medial halves of the somites. The lateral halves of the somites are derived from a separate region in the primitive streak, caudal to Hensen's node.

On Duplicitas Anterior in an Early Chick Embryo

1899 ◽  
Vol 22 ◽  
pp. 622-630 ◽  
Author(s):  
Thomas H. Bryce
Keyword(s):  
Chick Embryo ◽  
Small Proportion ◽  
Partial Information ◽  
Primitive Streak ◽  
Serial Sections ◽  
Early Chick Embryo ◽  
Stage 1

The literature of Duplicity in Birds affords, out of a total of about ninety-five recorded cases of multiple formations of all kinds on a single blastoderm, from the stage of the primitive streak to the fourth day of incubation, only a small proportion of instances of “duplicitas anterior.” Dareste (i.) in his atlas figures three; Gerlach (ii.) adds representations of three others—one case of his own, a second originally described by Ahlfeld, and a third by Reichert; Klaussner (iii.) gives a seventh case; and Bianchi (iv.) describes a monstrous embryo at a later stage (1°5 cm. in length).Most observers have been content with the partial information derived from the study of the whole object, and only three embryos of this class, which have been studied in serial sections, have been described:—1st. Erich Hoffman's (v.) with three somites.2nd. Mitrophanow's (vi.) with six somites.3rd. Kaestner's (vii.) with seven somites.

Metabolic Characteristics of the Heart-forming Areas of the Early Chick Embryo

Development ◽  
1957 ◽  
Vol 5 (4) ◽  
pp. 324-339
Author(s):  
Lowell M. Duffey ◽  
James D. Ebert
Keyword(s):  
Chick Embryo ◽  
Primitive Streak ◽  
Cardiac Myosin ◽  
Early Chick Embryo ◽  
Metabolic Characteristics ◽  
Process Stage ◽  
Cardiac Actin ◽  
Cardiac Contractile ◽  
Sequence Of Events ◽  
Cardiac Contractile Proteins

Our knowledge of the sequence of events that culminate in the onset of contracility in the heart of the early chick embryo has been evaluated by Ebert, Tolman, Mun, & Albright (1955). Immunochemical analyses made during the initial phases of cardiogenesis, which precede the appearance of recognizable cardiac primordia, indicate that in the embryo at the head-process stage the distribution of the proteins, cardiac myosin (Ebert, 1953), and cardiac actin (Ebert et al., 1955), coincides with the heart-forming areas as defined by isolation methods (Rawles, 1943). In earlier stages detectable quantities of cardiac actin are absent, and cardiac myosin is distributed throughout the epiblast in the embryo at the definitive primitive streak stage. Present concepts of the synthesis and distribution of the cardiac contractile proteins are based on the sensitivity of the immunochemical methods.

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