scholarly journals Control of imaginal cell development by the escargot gene of Drosophila

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 105-115 ◽  
Author(s):  
S. Hayashi ◽  
S. Hirose ◽  
T. Metcalfe ◽  
A.D. Shirras
Keyword(s):  
Cell Division ◽  
Zinc Finger ◽  
Imaginal Discs ◽  
Cell Development ◽  
Epidermal Cells ◽  
Normal Functions ◽  
Similar Phenotype ◽  
Snail Gene ◽  
Third Instar Larvae

Mutations in the escargot (esg) locus, which codes for a zinc-finger-containing protein with similarity to the product of the snail gene, cause a variety of defects in adult structures such as loss of abdominal cuticle and malformation of the wings and legs. esg RNA is expressed in wing, haltere, leg and genital imaginal discs and in abdominal histoblast nests in the embryo. Expression in imaginal tissues is also found in third instar larvae. In esg mutant larvae, normally diploid abdominal histoblasts replicate their DNA without cell division and become similar in appearance to the polytene larval epidermal cells. A similar phenotype was also found in imaginal discs of larvae mutant for both esg and the Drosophila raf gene. These results suggest that one of the normal functions of esg may be the maintenance of diploidy in imaginal cells.

Functions of promyelocytic leukaemia zinc finger (Plzf) in male germline stem cell development and differentiation

2019 ◽  
Vol 31 (8) ◽  
pp. 1315 ◽  
Author(s):  
Daguia Zambe John Clotaire ◽  
Yudong Wei ◽  
Xiuwei Yu ◽  
Tamgue Ousman ◽  
Jinlian Hua
Keyword(s):  
Stem Cells ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Development Stage ◽  
Cell Development ◽  
Promyelocytic Leukaemia ◽  
Germline Stem Cell ◽  
Germline Stem Cells ◽  
Male Germline ◽  
Finger Protein

Promyelocytic leukaemia zinc finger (Plzf), also known as zinc finger and BTB domain containing 16 (ZBTB16) or zinc-finger protein 145 (ZFP145), is a critical zinc finger protein of male germline stem cells (mGSCs). Multiple lines of evidence indicate that Plzf has a central role in the development, differentiation and maintenance of many stem cells, including mGSCs, and Plzf has been validated as an essential transcription factor for mammalian testis development and spermatogenesis. This review summarises current literature focusing on the significance of Plzf in maintaining and regulating self-renewal and differentiation of mGSCs, especially goat mGSCs. The review summarises evidence of the specificity of Plzf expression in germ cell development stage, the known functions of Plzf and the microRNA-mediated mechanisms that control Plzf expression in mGSCs.

The lymphoid transcription factor LyF-1 is encoded by specific, alternatively spliced mRNAs derived from the Ikaros gene

1994 ◽  
Vol 14 (11) ◽  
pp. 7111-7123
Author(s):  
K Hahm ◽  
P Ernst ◽  
K Lo ◽  
G S Kim ◽  
C Turck ◽  
...  
Keyword(s):  
Amino Acid ◽  
T Cell ◽  
Dna Binding ◽  
Amino Acid Sequence ◽  
Zinc Finger ◽  
T Cell Development ◽  
Cell Development ◽  
Control Element ◽  
Early Stages ◽  
Alternatively Spliced

The lymphocyte-specific DNA-binding protein LyF-1 interacts with a critical control element in the terminal deoxynucleotidyltransferase (TdT) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. We have purified LyF-1 and have obtained a partial amino acid sequence from proteolytic peptides. The amino acid sequence suggests that LyF-1 is a zinc finger protein encoded by the Ikaros gene, which previously was implicated in T-cell development. Recombinant Ikaros expressed in Escherichia coli bound to the TdT promoter, and antisera directed against the recombinant protein specifically blocked the DNA-binding activity of LyF-1 in crude extracts. Further analysis revealed that at least six distinct mRNAs are derived from the Ikaros/LyF-1 gene by alternative splicing. Only two of the isoforms possess the N-terminal zinc finger domain that is necessary and sufficient for TdT promoter binding. Although both of these isoforms bound to similar sequences in the TdT, lambda 5, VpreB, and lck promoters, one isoform contains an additional zinc finger that resulted in altered recognition of some binding sites. At least four of the Ikaros/LyF-1 isoforms were detectable in extracts from B- and T-cell lines, with the relative amounts of the isoforms varying considerably. These data reveal that the LyF-1 protein is encoded by specific mRNAs derived from the alternatively-spliced Ikaros gene, suggesting that this gene may be important for the early stages of both B- and T-lymphocyte development.

scholarly journals Advances in the Regulation of Epidermal Cell Development by C2H2 Zinc Finger Proteins in Plants

2021 ◽  
Vol 12 ◽  
Author(s):  
Guoliang Han ◽  
Yuxia Li ◽  
Ziqi Qiao ◽  
Chengfeng Wang ◽  
Yang Zhao ◽  
...  
Keyword(s):  
Cell Fate ◽  
Zinc Finger ◽  
Epidermal Cell ◽  
Stress Responses ◽  
Root Hairs ◽  
Zinc Finger Proteins ◽  
Cell Development ◽  
Salt Glands ◽  
Plant Adaptation ◽  
C2h2 Zinc Finger

Plant epidermal cells, such as trichomes, root hairs, salt glands, and stomata, play pivotal roles in the growth, development, and environmental adaptation of terrestrial plants. Cell fate determination, differentiation, and the formation of epidermal structures represent basic developmental processes in multicellular organisms. Increasing evidence indicates that C2H2 zinc finger proteins play important roles in regulating the development of epidermal structures in plants and plant adaptation to unfavorable environments. Here, we systematically summarize the molecular mechanism underlying the roles of C2H2 zinc finger proteins in controlling epidermal cell formation in plants, with an emphasis on trichomes, root hairs, and salt glands and their roles in plant adaptation to environmental stress. In addition, we discuss the possible roles of homologous C2H2 zinc finger proteins in trichome development in non-halophytes and salt gland development in halophytes based on bioinformatic analysis. This review provides a foundation for further study of epidermal cell development and abiotic stress responses in plants.

Cell cycle progression, growth and patterning in imaginal discs despite inhibition of cell division after inactivation of Drosophila Cdc2 kinase

Development ◽  
1997 ◽  
Vol 124 (18) ◽  
pp. 3555-3563 ◽  
Author(s):  
K. Weigmann ◽  
S.M. Cohen ◽  
C.F. Lehner
Keyword(s):  
Cell Division ◽  
Cell Cycle Progression ◽  
Positional Information ◽  
Imaginal Discs ◽  
Physical Distance ◽  
Cdc2 Kinase ◽  
Cycle Progression ◽  
Mitotic Kinase ◽  
Control Growth ◽  
Local Cell

During larval development, Drosophila imaginal discs increase in size about 1000-fold and cells are instructed to acquire distinct fates as a function of their position. The secreted signaling molecules Wingless and Decapentaplegic have been implicated as sources of positional information that globally control growth and patterning. Evidence has also been presented that local cell interactions play an important role in controlling cell proliferation in imaginal discs. As a first step to understanding how patterning cues influence growth we investigated the effects of blocking cell division at different times and in spatially controlled manner by inactivation of the mitotic kinase Cdc2 in developing imaginal discs. We find that cell growth continues after inactivation of Cdc2, with little effect on overall patterning. The mechanisms that regulate size of the disc therefore do not function by regulating cell division, but appear to act primarily by regulating size in terms of physical distance or tissue volume.

scholarly journals Ovol2, a zinc finger transcription factor, is dispensable for spermatogenesis in mice

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Jin Zhang ◽  
Juan Dong ◽  
Weibing Qin ◽  
Congcong Cao ◽  
Yujiao Wen ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Germ Cell ◽  
Zinc Finger ◽  
Germ Cells ◽  
Cell Development ◽  
Knockout Mouse Model ◽  
Zinc Finger Transcription Factor ◽  
Germ Cell Development ◽  
Embryonic Germ Cells ◽  
Protein Levels

AbstractOvol2, a mouse homolog of Drosophila ovo, was identified as a zinc finger transcription factor predominantly expressed in testis. However, the function of Ovol2 in postnatal male germ cell development remains enigmatic. Here, we firstly examined the mRNA and protein levels of Ovol2 in developing mouse testes by RT-qPCR and western blot and found that both mRNA and protein of Ovol2 are continually expressed in postnatal developing testes from postnatal day 0 (P0) testes to adult testes (P56) and exhibits its higher level at adult testis. Further testicular immuno-staining revealed that OVOL2 is highly expressed in the spermatogonia, spermatocytes and round spermatids. Interestingly, our conditional ovol2 knockout mouse model show that loss of ovol2 in embryonic germ cells does not affect fecundity in mice. Our data also show that Ovol1 may have compensated for the loss of Ovol2 functions in germ cells. Overall, our data indicate that ovol2 is dispensable for germ cell development and spermatogenesis.

scholarly journals Drosophila twin spot clones reveal cell division dynamics in regenerating imaginal discs

2011 ◽  
Vol 356 (2) ◽  
pp. 576-587 ◽  
Author(s):  
Anne Sustar ◽  
Marianne Bonvin ◽  
Margrit Schubiger ◽  
Gerold Schubiger
Keyword(s):  
Cell Division ◽  
Imaginal Discs

Fine structure of Wolffia arrhiza

1973 ◽  
Vol 51 (9) ◽  
pp. 1619-1622 ◽  
Author(s):  
J. L. Anderson ◽  
W. W. Thomson ◽  
J. A. Swader
Keyword(s):  
Fine Structure ◽  
Cell Division ◽  
Vegetative Reproduction ◽  
Guard Cells ◽  
Epidermal Cells ◽  
Electron Microscopic ◽  
Meristematic Cells ◽  
Wolffia Arrhiza ◽  
Microscopic Studies

Light and electron microscopic studies of Wolffia arrhiza L. frond development during vegetative reproduction showed that the fronds were composed entirely of chlorenchymous cells. Chloroplasts in the epidermal cells other than the guard cells were unique in that they contained no starch. Cell division occurred only at the proximal end of daughter fronds early in their development. Meristematic cells contained chloroplasts with clearly defined grana. Proplastids, commonly observed in meristematic cells of apical regions of other plants, were absent in the cells of these plants.

Divisions cellulaires au niveau de l'épiderme de l'hypocotyle du lin (Linum usitatissimum) cultivé in vitro

1985 ◽  
Vol 63 (10) ◽  
pp. 1691-1695 ◽  
Author(s):  
M. Sqalli ◽  
H. Chlyah
Keyword(s):  
Cell Division ◽  
Culture Cell ◽  
Epidermal Cells ◽  
Isolated Cells ◽  
Initiation And Propagation ◽  
A Cell ◽  
The Mean ◽  
Electron Microscopes ◽  
Scanning Electron Microscopes

A study of the initiation and propagation of cell divisions in the epidermis of flax hypocotyl segments cultured in vitro was made using surface observations (light and scanning electron microscopes) as well as transverse and longitudinal sections. Epidermal cells were of two types: long, narrow cells and short, wide cells. The latter, less numerous, rarely participated in cell division. Nuclear activation and the first mitoses appeared very early (after 4–8 h of culture). Cell division began in isolated cells and spread progressively to surrounding cells arranged transversely. At 24 h, approximately 50 cells in division or newly divided were observed on an epidermal strip of 10 × 2 mm composed of about 8000 original cells. At 48 h, about 110 cells had divided forming 22 division centers; 26 prophase, 10 metaphase, and 7 telophase figures were observed. The mean number of original cells which participated in the formation of a cell division center was three at 12 h, five at 72 h, with no increase thereafter. The percentage of cells in mitosis or already divided remained low (1.9%) in relation to the total number of epidermal cells. For 22 division centers, only 7 would participate in vegetative bud formation.

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