scholarly journals Positional cues governing cell migration in leech neurogenesis

Development ◽  
1991 ◽  
Vol 111 (4) ◽  
pp. 993-1005 ◽  
Author(s):  
S.A. Torrence
Keyword(s):  
Nervous System ◽  
Cell Line ◽  
Cell Lines ◽  
Embryonic Cell ◽  
Long Distance ◽  
Mesodermal Cell ◽  
Selective Ablation ◽  
Embryonic Cell Lines ◽  
Migration Pathways ◽  
Glial Precursor

The stereotyped distribution of identified neurons and glial cells in the leech nervous system is the product of stereotyped cell migrations and rearrangements during embryogenesis. To examine the dependence of long-distance cell migrations on positional cues provided by other tissues, embryos of Theromyzon rude were examined for the effects of selective ablation of various embryonic cell lines on the migration and final distribution of neural and glial precursor cells descended from the bilaterally paired ectodermal cell lines designated q bandlets. The results suggest that neither the commitment of q-bandlet cells to migrate nor the general lateral-to-medial direction of their migration depend on interactions with any other cell line. However, the ability of the migrating cells to follow their normal pathways and to find their normal destinations does depend on interactions with cells of the mesodermal cell line, which appears to provide positional cues that specify the migration pathways.

Development and validation of swine embryonic stem cells: a review

1994 ◽  
Vol 6 (5) ◽  
pp. 563 ◽  
Author(s):  
MB Wheeler
Keyword(s):  
Cell Lines ◽  
Es Cells ◽  
Embryonic Stem ◽  
Morphological Differentiation ◽  
Cell Types ◽  
Embryonic Cell ◽  
Embryoid Bodies ◽  
Mesodermal Cell ◽  
Diploid Complement ◽  
Embryonic Cell Lines

The establishment of embryonic cell lines from swine should be useful for studies of cell differentiation, developmental gene regulation and the production of transgenics. This paper summarizes the establishment of porcine (Sus scrofa) embryonic stem (ES) cell lines from preimplantation blastocysts and their ability to develop into normal chimaeras. ES cells can spontaneously differentiate into cystic embryoid bodies with ectodermal, endodermal, and mesodermal cell types. Further, culture of ES cells to confluence or induction of differentiation with retinoic acid or dimethylsulfoxide results in morphological differentiation into fibroblasts, adipocytes, and epithelial, neuronal, and muscle cells. These ES cells have a normal diploid complement of 38 chromosomes. Scanning electron microscopy of the ES cells reveals a rounded or polygonal, epithelial-like cell with numerous microvilli. The differentiation of these embryonic cell lines into several cell types indicates a pluripotent cell. Furthermore, chimaeric swine have been successfully produced using such ES cells.

Isolation of embryonic cell‐lines from porcine blastocysts

1995 ◽  
Vol 6 (1) ◽  
pp. 1-14 ◽  
Author(s):  
R.W. Gerfen ◽  
M.B. Wheeler
Keyword(s):  
Cell Lines ◽  
Embryonic Cell ◽  
Embryonic Cell Lines

Transcriptional regulation of the murine k-FGF gene in embryonic cell lines

1992 ◽  
Vol 154 (1) ◽  
pp. 45-54 ◽  
Author(s):  
Yan-Gao Ma ◽  
Edward Rosfjord ◽  
Claire Huebert ◽  
Phillip Wilder ◽  
Jay Tiesman ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Cell Lines ◽  
Embryonic Cell ◽  
Embryonic Cell Lines

scholarly journals Derivation of pluripotent, embryonic cell lines from the pig and sheep

2019 ◽  
Author(s):  
Elena Notarianni ◽  
C Galli ◽  
S Laurie ◽  
RM Moor ◽  
MJ Evans
Keyword(s):  
Cell Lines ◽  
Embryonic Cell ◽  
Embryonic Cell Lines

scholarly journals HIPPIE2: a method for fine-scale identification of physically interacting chromatin regions

2019 ◽  
Author(s):  
Pavel P. Kuksa ◽  
Alexandre Amlie-Wolf ◽  
Yih-Chii Hwang ◽  
Otto Valladares ◽  
Brian D. Gregory ◽  
...  
Keyword(s):  
High Resolution ◽  
Cell Line ◽  
Cell Lines ◽  
Dna Looping ◽  
Open Chromatin ◽  
Fine Scale ◽  
Long Distance ◽  
Chromosome Conformation ◽  
Chromatin Interactions ◽  
Distance Regulation

AbstractMost regulatory chromatin interactions are mediated by various transcription factors (TFs) and involve physically-interacting elements such as enhancers, insulators, or promoters. To map these elements and interactions, we developed HIPPIE2 which analyzes raw reads from high-throughput chromosome conformation (Hi-C) experiments to identify fine-scale physically-interacting regions (PIRs). Unlike standard genome binning approaches (e.g., 10K-1Mbp bins), HIPPIE2 dynamically calls physical locations of PIRs with better precision and higher resolution based on the pattern of restriction events and relative locations of interacting sites inferred from the sequencing readout.We applied HIPPIE2 to in situ Hi-C datasets across 6 human cell lines (GM12878, IMR90, K562, HMEC, HUVEC, NHEK) with matched ENCODE and Roadmap functional genomic data. HIPPIE2 detected 1,042,738 distinct PIRs across cell lines, with high resolution (average PIR length of 1,006bps) and high reproducibility (92.3% in GM12878 replicates). 32.8% of PIRs were shared among cell lines. PIRs are enriched for epigenetic marks (H3K27ac, H3K4me1) and open chromatin, suggesting active regulatory roles. HIPPIE2 identified 2.8M significant intrachromosomal PIR–PIR interactions, 27.2% of which were enriched for TF binding sites. 50,608 interactions were enhancer–promoter interactions and were enriched for 33 TFs (31 in enhancers/29 in promoters), several of which are known to mediate DNA looping/long-distance regulation. 29 TFs were enriched in >1 cell line and 4 were cell line-specific. These findings demonstrate that the dynamic approach used in HIPPIE2 (https://bitbucket.com/wanglab-upenn/HIPPIE2) characterizes PIR–PIR interactions with high resolution and reproducibility.

Cell Dynamics in Early Embryogenesis and Pluripotent Embryonic Cell Lines: From Sea Urchin to Mammals

2009 ◽  
pp. 215-244 ◽  
Author(s):  
Anne-Marie Genevière ◽  
Antoine Aze ◽  
Yasmine Even ◽  
Maria Imschenetzky ◽  
Clara Nervi ◽  
...  
Keyword(s):  
Cell Lines ◽  
Sea Urchin ◽  
Embryonic Cell ◽  
Early Embryogenesis ◽  
Cell Dynamics ◽  
Embryonic Cell Lines
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