Regulations in the induction of the organized neural system in amphibian embryos

Development ◽  
1990 ◽  
Vol 110 (3) ◽  
pp. 653-659 ◽  
Author(s):  
T. Yamada
Keyword(s):  
Homeobox Gene ◽  
Internal Surface ◽  
Neural System ◽  
The Body ◽  
Convergent Extension ◽  
Kinase C ◽  
Amphibian Embryos ◽  
Series Of Experiments ◽  
System 1 ◽  
Posterior Anterior

Some of the recent data on the induction of the neural system in amphibian embryos are reviewed, utilizing a model, according to which two basic events regulate in this system: (1) ectodermal dorsalization, which occurs all over the induced region of the ectoderm and is responsible for the neural and mesectodermal pathways and (2) caudalization, which occurs only on the posterior level of dorsalized ectoderm and is responsible for the posterior mode of induced differentiation, functioning as a gradient with the apex at the posterior end of the embryo. Dorsalization of ectoderm can be caused by treatment with Con A or TPA, both of which are potential mitogens. Not only after the treatment with TPA, but also during normal dorsalization, the activation of protein kinase C occurs in responding cells. The possibility is suggested that an early step of mitogenic transmembrane signal transduction induced by a growth factor regulates dorsalization in intact embryos. Ectodermal dorsalization is responsible for the appearance of neuronal and glial cell lineages, and independent of the ECM network formed on the internal surface of the responding ectoderm during gastrulation. In caudalization, a series of experiments suggests that the regulatory role is played by the transcript of the mesodermal posterior homeobox gene, Xhox 3. The expression of this gene in time and location closely coincides with the pattern of convergent extension, one type of morphogenetic movement, which is expressed in a posterior-anterior gradient. This directed cell motility is responsible for the formation of the body axis of vertebrates, and was shown to be involved in caudalization by earlier induction experiments in urodele embryos.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Nedd4-2 but not Nedd4-1 is critical for protein kinase C-regulated ubiquitination, expression, and transport activity of human organic anion transporter 1

2016 ◽  
Vol 310 (9) ◽  
pp. F821-F831 ◽  
Author(s):  
Da Xu ◽  
Haoxun Wang ◽  
Qiang Zhang ◽  
Guofeng You
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Cell Surface ◽  
Organic Anion ◽  
The Body ◽  
Organic Anion Transporter ◽  
Transport Activity ◽  
Kinase C ◽  
Anion Transporter ◽  
In Cells

Human organic anion transporter 1 (hOAT1) expressed at the membrane of the kidney proximal tubule cells mediates the body disposition of a diverse array of clinically important drugs, including anti-HIV therapeutics, antitumor drugs, antibiotics, antihypertensives, and antiinflammatories. Therefore, understanding the regulation of hOAT1 will provide significant insights into kidney function and dysfunction. We previously established that hOAT1 transport activity is inhibited by activation of protein kinase C (PKC) through accelerating hOAT1 internalization from cell surface into intracellular endosomes and subsequent degradation. We further established that PKC-induced hOAT1 ubiquitination is an important step preceding hOAT1 internalization. In the current study, we identified two closely related E3 ubiquitin ligases, neural precursor cell expressed, developmentally downregulated 4-1 and 4-2 (Nedd4-1 and Nedd4-2), as important regulators for hOAT1: overexpression of Nedd4-1 or Nedd4-2 enhanced hOAT1 ubiquitination, reduced the hOAT1 amount at the cell surface, and suppressed hOAT1 transport activity. In further exploring the relationship among PKC, Nedd4-1, and Nedd4-2, we discovered that PKC-dependent changes in hOAT1 ubiquitination, expression, and transport activity were significantly blocked in cells transfected with the ligase-dead mutant of Nedd4-2 (Nedd4-2/C821A) or with Nedd4-2-specific siRNA to knockdown endogenous Nedd4-2 but not in cells transfected with the ligase-dead mutant of Nedd4-1 (Nedd4-1/C867S) or with Nedd4-1-specific siRNA to knockdown endogenous Nedd4-1. In conclusion, this is the first demonstration that both Nedd4-1 and Nedd4-2 are important regulators for hOAT1 ubiquitination, expression, and function. Yet they play distinct roles, as Nedd4-2 but not Nedd4-1 is a critical mediator for PKC-regulated hOAT1 ubiquitination, expression, and transport activity.

Aluminum coatings on the internal surface of the body of color TV tubes

1990 ◽  
Vol 25 (4) ◽  
pp. 432-435
Author(s):  
B. S. Malkiel' ◽  
A. R. Gachkevich ◽  
Yu. R. Sosnovyi ◽  
R. F. Terletskii ◽  
G. V. Pokhumurskaya
Keyword(s):  
Internal Surface ◽  
The Body ◽  
Aluminum Coatings

κ- but not δ-opioid receptors mediate effects of ischemic preconditioning on both infarct and arrhythmia in rats

2001 ◽  
Vol 280 (1) ◽  
pp. H384-H391 ◽  
Author(s):  
Guan-Ying Wang ◽  
Song Wu ◽  
Jian-Ming Pei ◽  
Xiao-Chun Yu ◽  
Tak-Ming Wong
Keyword(s):  
Infarct Size ◽  
Opioid Receptors ◽  
Ischemic Preconditioning ◽  
Antiarrhythmic Action ◽  
Perfused Rat Heart ◽  
Calphostin C ◽  
Kinase C ◽  
Series Of Experiments ◽  
Pkc Inhibitors

Two series of experiments were performed in the isolated perfused rat heart to determine the role of κ- and δ-opioid receptors (OR) in cardioprotection of ischemic preconditioning (IP). In the first series of experiments, it was found that IP with two cycles of 5-min regional ischemia followed by 5-min reperfusion each reduced infarct size induced by 30-min ischemia, and the ameliorating effect of IP on infarct was attenuated with blockade of either 5 × 10−6 mol/l nor-binaltorphimine (nor-BNI), a selective κ-OR antagonist, or 5 × 10−6 mol/l naltrindole (NTD), a selective δ-OR antagonist. The second series showed that U50,488H, a selective κ-OR agonist, ord-Ala2-d-leu5-enkephalin (DADLE), a selective δ-OR agonist, dose dependently reduced the infarct size induced by ischemia, which mimicked the effects of IP. The effect of 10−5 mol/l U50,488H on infarct was significantly attenuated by blockade of protein kinase C (PKC) with specific PKC inhibitors, 5 × 10−6 mol/l chelerythrine or 8 × 10−7 mol/l calphostin C, as well as by blockade of ATP-sensitive K+ (KATP) channels with blockers of the channel, 10−5 mol/l glibenclamide or 10−4 mol/l 5-hydroxydecanoate. IP also reduced arrhythmia induced by ischemia. Nor-BNI, but not NTD, attenuated, while U50,488H, but not DADLE, mimicked the antiarrhythmic action of IP. In conclusion, the present study has provided first evidence that κ-OR mediates the ameliorating effects of IP on infarct and arrhythmia induced by ischemia, whereas δ-OR mediates the effects only on infarct. Both PKC and KATP channels mediate the effect of activation of κ-OR on infarct.

scholarly journals Morphological characteristics of respiratory and digestive organs of Roman snail (Helix pomatia L., 1758)

2020 ◽  
Vol 22 (97) ◽  
pp. 7-12
Author(s):  
M. P. Horvat ◽  
R. S. Dankovych
Keyword(s):  
Helix Pomatia ◽  
Morphological Characteristics ◽  
Internal Surface ◽  
The Body ◽  
Digestive Cells ◽  
Green Color ◽  
Excretory Function ◽  
Intracellular Digestion ◽  
Snail Helix Pomatia ◽  
Calcium Cells

The aim of this work was to study the structure of lung and hepatopancreas of Roman snail (Helix of pomatia of L., 1758). The study found that the lung occupies the lower turn of shell and presented by a saccate cavity, in the wall of that there are a kidney and heart with a pericardium, and also a rectum and ureter pass. An external surface of lungs covered by a shell and covered by an epidermis. An internal surface is covered by a flat ciliated epithelium and forms numerous folds in which pulmonary vessels and lacunae are accommodated. The branches of pulmonary vein have a thick muscular wall, that consists of circular and longitudinal muscular layers. An internal surface of lungs covered by the layer of mucus. Inhalation and exhalation are carried out due to reduction and relaxation of muscles of dorsal wall of the body that is named a “diaphragm”. Gas exchange occurs through the hemolymphatic capillaries of the lung wall. Respiratory motions take place not rhythmically, but through the different intervals of time depending on a requirement in oxygen. The frequency of pneumostome closing and opening is typically one time in a minute. At subzero humidity of atmospheric air of pneumostome closed by a mantle, and also one (or a few) epiphragms. The hepatopancreas (“liver” or liver gland) is in the upper rotation of the sink and formed by two parts: right and left, from which two liver ducts enter into the stomach respectively. The liver gland consists of many acinuss, surrounded by connecting tissue, that contains small number of muscular fibres. Calcium cells have a pyramidal form and usually do not reach the lumen of the acinus. Cytoplasm of calcium cells contains inclusions: grains of phosphoricacid lime and drops of fat. The digestive cells of the hepatopencreas are more elongated, often clavicular. Сytoplasm of digestive cells is loose and vacuolated and contain inclusions of yellow-green color. Enzyme cells on histopreparations are difficult to distinguish from digestive ones. They contain transparent vacuoles with a large round inclusion of yellow-green color, which consists of a cluster of several grains of different sizes. Hepatopancreas performs the following functions: secretory (enzyme cells), absorption and intracellular digestion (digestive cells), preservation of nutrients and calcium (calcium cells), and also excretory function.

Proton Dose Conversion Coefficients Based on Chinese Reference Adult Woman Voxel Phantom

2013 ◽  
Author(s):  
Fangfang Liu ◽  
Mingqi Shen ◽  
Taosheng Li ◽  
Chunyu Liu
Keyword(s):  
Effective Dose ◽  
Reference Data ◽  
Absorbed Dose ◽  
The Body ◽  
Adult Woman ◽  
Voxel Model ◽  
Conversion Coefficients ◽  
Computational Phantoms ◽  
Posterior Anterior ◽  
Anterior Posterior

In order to calculate the dose conversion coefficients for proton, the voxel model of Chinese Reference Adult Woman (CRAW) was established by the Monte Carlo transport code FLUKA according to the Chinese reference data and the Asian reference data. Compared with the reference data, the deviations of the mass for organs or tissues of CRAW is less than ±5%. Calculations have been performed for 14 incident monoenergetic protons energies from 0.02GeV to 10TeV at the irradiation incident of anterior-posterior (AP) and posterior-anterior (PA). The results of fluence-to-effective dose conversion coefficients are compared with data from the different models such as an anthropomorphic mathematical model, ICRP reference adult voxel model, the voxel-based visible Chinese human (VCH). Anatomical differences among various computational phantoms and the spatial geometric positions of the organs or tissues lead to the discrepancies of the effective dose conversion coefficients in the ranging from a negligible level to 107% at proton energies below 0.2GeV. The deviations of the coefficients, above 0.2GeV, are mostly within 10%. The results of fluence-to-organ absorbed dose conversion coefficients are compared with the data of VCH. The deviations of the coefficients, below and above 0.2GeV, are within 150% and 20%, respectively. The primary factors of the deviations for the coefficients should be due to the differences of the organ mass and the size of the body shape.

Triadic body representations in the human cerebral cortex and peripheral nerves

2021 ◽  
pp. 133-151 ◽  
Author(s):  
Noriaki Kanayama ◽  
Kentaro Hiromitsu
Keyword(s):  
Peripheral Nerves ◽  
Sensory Modality ◽  
Body Schema ◽  
Body Representation ◽  
Neural System ◽  
The Body ◽  
Neural Representation ◽  
Structural Description ◽  
Research Fields ◽  
The Brain

Is the body reducible to neural representation in the brain? There is some evidence that the brain contributes to the functioning of the body from neuroimaging, neurophysiological, and lesion studies. Well-known dyadic taxonomy of the body schema and the body image (hereafter BSBI) is based primarily on the evidence in brain-damaged patients. Although there is a growing consensus that the BSBI exists, there is little agreement on the dyadic taxonomy because it is not a concrete and common concept across various research fields. This chapter tries to investigate the body representation in the cortex and nervous system in terms of sensory modality and psychological function using two different approaches. The first approach is to review the neurological evidence and cortical area which is related to body representation, regardless of the BSBI, and then to reconsider how we postulate the BSBI in our brain. It can be considered that our body representation could be constructed by the whole of the neural system, including the cortex and peripheral nerves. The second approach is to revisit the BSBI conception from the viewpoint of recent neuropsychology and propose three types of body representation: body schema, body structural description, and body semantics. This triadic taxonomy is considered consistent with the cortical networks based on the evidence of bodily disorders due to brain lesions. These two approaches allow to reconsider the BSBI more carefully and deeply and to give us the possibility that the body representation could be underpinned with the network in the brain.

Modulation of bat wing venule contraction by transmural pressure changes

1992 ◽  
Vol 262 (3) ◽  
pp. H625-H634 ◽  
Author(s):  
M. J. Davis ◽  
X. Shi ◽  
P. J. Sikes
Keyword(s):  
Venous Pressure ◽  
Transmural Pressure ◽  
The Body ◽  
Intraluminal Pressure ◽  
Positive Pressure ◽  
Cross Sectional ◽  
Series Of Experiments ◽  
Pressure Changes ◽  
Bat Wing

We tested the hypothesis that the frequency and amplitude of spontaneous venular contractions in the bat wing could be modulated by changes in transmural pressure. In one series of experiments, venous pressure in the wing was elevated by pressurizing a box containing the body of the animal while the wing was exposed to atmospheric pressure. During this time, venular diameters were continuously recorded using intravital microscopic techniques while venular pressures were measured through servo-null micropipettes. In another series of experiments, single venular segments were dissected from the wing, cannulated, and pressurized in vitro. The results from both experimental protocols were qualitatively similar; alterations in venous pressure over a narrow range (+/- 5 cmH2O from control) produced substantial changes in contraction frequency and amplitude. The product of frequency and cross-sectional area was maximal over the venous pressure range between 10 and 15 cmH2O. Venules demonstrated a rate-sensitive component in their reaction to rapid pressure changes, because contraction bursts occurred immediately after positive pressure steps and quiescent periods often occurred after negative pressure steps. We conclude that venular vasomotion in the bat wing is modulated by intraluminal pressure and involves a bidirectional, rate-sensitive mechanism. In addition, comparisons with arteriolar vasomotion studies suggest that venules are more sensitive to luminal pressure changes than arterioles.

Independence of brain and tympanic temperatures in an unanesthetized human

1988 ◽  
Vol 65 (1) ◽  
pp. 482-486 ◽  
Author(s):  
K. Shiraki ◽  
S. Sagawa ◽  
F. Tajima ◽  
A. Yokota ◽  
M. Hashimoto ◽  
...  
Keyword(s):  
Skin Surface ◽  
The Body ◽  
Tympanic Temperature ◽  
The Face ◽  
Series Of Experiments ◽  
Long Term Monitoring ◽  
The Brain ◽  
Term Monitoring ◽  
Monitoring Temperature

Temperature within the brain and the esophagus and at the tympanum were obtained in a 12-yr-old male in a series of experiments that began 8 days after surgery for implantation of a drainage catheter. Fanning the face did reduce tympanic temperature but not temperature in the brain; brain temperatures followed esophageal temperatures. In long-term monitoring, temperature in the lateral ventricle was 0.5 degree C above esophageal temperature and 0.2 degree C below that in white matter 1 cm above, with the offsets fixed throughout the overnight cycle. All temperatures went through similar excursions when the face was excluded from fanning applied to the body. These observations highlight the fact that in humans the defense against hyperthermia takes advantage of cooling distributed over the entire skin surface.

Growth and development of water buffalo and Friesian cross-bred cattle, with special reference to growth and distribution of carcass muscle and bone

1982 ◽  
Vol 98 (2) ◽  
pp. 317-323 ◽  
Author(s):  
O. Y. Abdallah ◽  
Karima A. Shahin ◽  
M. G. A. Latif
Keyword(s):  
Growth And Development ◽  
Water Buffalo ◽  
Carcass Composition ◽  
The Body ◽  
Trunk Muscles ◽  
Allometric Growth ◽  
Muscle Weight ◽  
Reverse Pattern ◽  
Total Muscle ◽  
Posterior Anterior

SUMMARYRight sides of carcasses of 12 buffalo, nine ½ Friesian ( Friesian x Egyptian Baladi) and nine ¾ Friesian ( Friesian x ½ Friesian) bulls slaughtered over the body-weight ranges 161–560 kg for buffaloes and 176–448 kg for cattle were cut according to the Chicago style of cutting. Allometric growth pattern and distribution of muscle and bone in the various cuts were examined.Buffaloes were similar to cattle in that muscle and bone in the limbs showed an increasing distoproximal growth gradient. On the ventral line of the trunk, muscles showed an increase in growth impetus from plate to brisket while bone followed a reverse pattern. In buffaloes, growth coefficients of muscle and bone of all dorsal cuts were similar except in the sirloin and chuck where bone was later maturing. In cattle, there appeared a posterior-anterior increase in growth impetus of muscle from the shortloin towards sticking. Within and between genotype-group results indicated that growth of a fatless cut is governed by the growth of its muscle rather than that of its bone.Compared with Friesian crosses, buffaloes had significantly greater percentages of their total muscle and muscle-plus-bone in the expensive cuts. As total muscle weight increased, the proportion of muscle in BLRC (see p. 318) decreased in cattle but remained almost constant in buffaloes. It was concluded that the previously reported inferior carcass composition of buffaloes relative to cattle is compensated by better carcass conformation and tissue distribution of buffaloes.

scholarly journals Kill Line Model Cross Flow Inline Coupled Vortex-Induced Vibration

2017 ◽  
Author(s):  
Baiheng Wu ◽  
Jorlyn Le Garrec ◽  
Dixia Fan ◽  
Michael S. Triantafyllou
Keyword(s):  
Bluff Body ◽  
Oil And Gas ◽  
Cross Flow ◽  
The Body ◽  
Bluff Bodies ◽  
Structural Vibrations ◽  
Vortex Induced Vibrations ◽  
Series Of Experiments ◽  
Central Pipe ◽  
Semi Empirical

Currents and waves cause flow-structure interaction problems in systems installed in the ocean. Particularly for bluff bodies, vortices form in the body wake, which can cause strong structural vibrations (Vortex-Induced Vibrations, VIV). The magnitude and frequency content of VIV is determined by the shape, material properties, and size of the bluff body, and the nature and velocity of the oncoming flow. Riser systems are extensively used in the ocean to drill for oil wells, or produce oil and gas from the bottom of the ocean. Risers often consist of a central pipe, surrounded by several smaller cylinders, including the kill and choke lines. We present a series of experiments involving forced in-line and cross flow motions of short rigid sections of a riser containing 6 symmetrically arranged kill and choke lines. The experiments were carried out at the MIT Towing Tank. We present a systematic database of the hydrodynamic coefficients, consisting of the forces in phase with velocity and the added mass coefficients that are also suitable to be used with semi-empirical VIV predicting codes.

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