Experimental Studies on the Origin of the Cartilage of the Auditory Capsule and Columella in Ambystoma
On the basis of earlier experimental results on amphibia and his own observations, Yntema (1955) came to the conclusion that the ‘ear rudiment or the ectoderm surrounding it may be a source of mesectoderm for the ear capsule, at least in the heterotopic position’. This is contrary to another view that the source of the cartilaginous ear capsule is of purely mesentodermal origin. The literature pertaining to these two points of view is reviewed by Yntema (1955) and Benoit (1957). The purpose of the present investigation is to study further the extent of the ectomesenchymal contribution to the auditory capsule in the orthotopic position. Ambystoma punctatum larvae were raised from eggs sent to this institute by Mr Glenn Gentry of Donelson, Tennessee. Some of the eggs were stained with Nile blue sulphate according to the method described by Detwiler (1917).