DNA Synthesis and Nuclear Reproduction during Embryonic Development and Regeneration of Muscle Tissue

Development ◽  
1963 ◽  
Vol 11 (2) ◽  
pp. 353-367
Author(s):  
L. N. Zhinkin ◽  
L. F. Andreeva
Keyword(s):  
Embryonic Development ◽  
Dna Synthesis ◽  
Muscle Tissue ◽  
Muscle Fibres ◽  
Muscle Proteins ◽  
Mode Of Reproduction ◽  
Skeletal Musculature ◽  
Developing Muscle ◽  
Morphological Investigations

Despite a large number of investigations devoted to the development and regeneration of skeletal musculature, the problem of the mode of reproduction of the muscle nuclei remains unsolved (Boyd, 1960; Murray, 1960; Holtzer, 1961; and many others). The majority of investigators believe that the symplast nuclei reproduce by amitosis (Bücher, 1959). Only a few investigations have shown the presence of mitoses in developing muscle fibres. Purely morphological investigations of the development and regeneration of muscle tissue seem to be unable to solve this problem. The nuclei of muscle fibres developing in vitro have recently been shown to synthesize DNA, moreover, the experiments showed the synthesis of DNA by the nuclei to be antagonistic to that of specialized muscle proteins (Stockdale & Holtzer, 1961). Bintliff & Walker (1960) showed that a considerable percentage of de-differentiated nuclei synthesized DNA upon regeneration of the skeletal musculature.

The actions of depolarizing drugs and antagonists on developing muscle fibres in vitro

1971 ◽  
Vol 23 (S1) ◽  
pp. 228S-228S ◽  
Author(s):  
WILLIAM F. DRYDEN ◽  
SOLOMON D. ERULKAR ◽  
GABRIEL HABA
Keyword(s):  
Muscle Fibres ◽  
Developing Muscle

Cell Transplantation for Myocardial Repair: An Experimental Approach

1992 ◽  
Vol 1 (6) ◽  
pp. 383-390 ◽  
Author(s):  
Daniel Marelli ◽  
Carolyne Desrosiers ◽  
Mohamed El-Alfy ◽  
Race L. Kao ◽  
Ray C.-J. Chiu
Keyword(s):  
Muscle Tissue ◽  
Satellite Cells ◽  
Tritiated Thymidine ◽  
Test Site ◽  
Scar Tissue ◽  
Control Site ◽  
Muscle Fibres ◽  
Myocardial Repair ◽  
Tibialis Muscle

Myocardium lacks the ability to regenerate following injury. This is in contrast to skeletal muscle (SKM), in which capacity for tissue repair is attributed to the presence of satellite cells. It was hypothesized that SKM satellite cells multiplied in vitro could be used to repair injured heart muscle. Fourteen dogs underwent explantation of the anterior tibialis muscle. Satellite cells were multiplied in vitro and their nuclei were labelled with tritiated thymidine 24 h prior to implantation. The same dogs were then subjected successfully to a myocardial injury by the application of a cryoprobe. The cells were suspended in serum-free growth medium and autotransplanted within the damaged muscle. Medium without cells was injected into an adjacent site to serve as a control. Endpoints comprised histology using standard stains as well as Masson trichrome (specific for connective tissue), and radioautography. In five dogs, satellite cell isolation, culture, and implantation were technically satisfactory. In three implanted dogs, specimens were taken within 6-8 wk. There were persistence of the implantation channels in the experimental sites when compared to the controls. Macroscopically, muscle tissue completely surrounded by scar tissue could be seen. Masson trichrome staining showed homogeneous scar in the control site, but not in the test site where a patch of muscle fibres containing intercalated discs (characteristic of myocardial tissue) was observed. In two other dogs, specimens were taken at 14 wk postimplantation. Muscle tissue could not be found. These preliminary results could be consistent with the hypothesis that SKM satellite cells can form neo-myocardium within an appropriate environment. Our specimens failed to demonstrate the presence of myocyte nuclei. It is therefore further hypothesized that in the late postoperative period, the muscle regenerate failed to survive.

scholarly journals Controllable assembly of skeletal muscle-like bundles through 3D bioprinting

2021 ◽  
Author(s):  
Tingting Fan ◽  
Shuo Wang ◽  
Zongmin Jiang ◽  
Shen Ji ◽  
Wenhua Cao ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
3D Printing ◽  
Muscle Tissue ◽  
Muscle Cells ◽  
Skeletal Muscle Tissue ◽  
Skeletal Muscle Cells ◽  
Muscle Fibres ◽  
Physical Factors

Abstract 3D printing is an effective technology for recreating skeletal muscle tissue in vitro. To achieve clinical skeletal muscle injury repair, relatively large volumes of highly aligned skeletal muscle cells are required; obtaining these is still a challenge. It is currently unclear how individual skeletal muscle cells and their neighbouring components co-ordinate to establish anisotropic architectures in highly homogeneous orientations. Here, we demonstrated a 3D printing strategy followed by sequential culture processes to engineer skeletal muscle tissue. The effects of confined printing on the skeletal muscle during maturation, which impacted the myotube alignment, myogenic gene expression, and mechanical forces, were observed. Our findings demonstrate the dynamic changes of skeletal muscle tissue during in vitro 3D construction and reveal the role of physical factors in the orientation and maturity of muscle fibres.

Complement (C5b-9) induces DNA synthesis in rat mesangial cells in vitro

2001 ◽  
Vol 59 (3) ◽  
pp. 905-912
Author(s):  
William G. Couser ◽  
Jeffrey W. Pippin ◽  
Stuart J. Shankland
Keyword(s):  
Dna Synthesis ◽  
Mesangial Cells

scholarly journals Recycled algae-based carbon materials as electroconductive 3D printed skeletal muscle tissue engineering scaffolds

2021 ◽  
Vol 32 (7) ◽  
Author(s):  
Selva Bilge ◽  
Emre Ergene ◽  
Ebru Talak ◽  
Seyda Gokyer ◽  
Yusuf Osman Donar ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Electrical Stimulation ◽  
Muscle Tissue ◽  
Carbonaceous Material ◽  
Hydrothermal Carbonization ◽  
Skeletal Muscle Tissue ◽  
Myotube Formation ◽  
3D Printed

AbstractSkeletal muscle is an electrically and mechanically active tissue that contains highly oriented, densely packed myofibrils. The tissue has self-regeneration capacity upon injury, which is limited in the cases of volumetric muscle loss. Several regenerative therapies have been developed in order to enhance this capacity, as well as to structurally and mechanically support the defect site during regeneration. Among them, biomimetic approaches that recapitulate the native microenvironment of the tissue in terms of parallel-aligned structure and biophysical signals were shown to be effective. In this study, we have developed 3D printed aligned and electrically active scaffolds in which the electrical conductivity was provided by carbonaceous material (CM) derived from algae-based biomass. The synthesis of this conductive and functional CM consisted of eco-friendly synthesis procedure such as pre-carbonization and multi-walled carbon nanotube (MWCNT) catalysis. CM obtained from biomass via hydrothermal carbonization (CM-03) and its ash form (CM-03K) were doped within poly(ɛ-caprolactone) (PCL) matrix and 3D printed to form scaffolds with aligned fibers for structural biomimicry. Scaffolds were seeded with C2C12 mouse myoblasts and subjected to electrical stimulation during the in vitro culture. Enhanced myotube formation was observed in electroactive groups compared to their non-conductive counterparts and it was observed that myotube formation and myotube maturity were significantly increased for CM-03 group after electrical stimulation. The results have therefore showed that the CM obtained from macroalgae biomass is a promising novel source for the production of the electrically conductive scaffolds for skeletal muscle tissue engineering.

scholarly journals Nobiletin enhances the development and quality of bovine embryos in vitro during two key periods of embryonic genome activation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karina Cañón-Beltrán ◽  
Yulia N. Cajas ◽  
Serafín Peréz-Cerezales ◽  
Claudia L. V. Leal ◽  
Ekaitz Agirregoitia ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Mitochondrial Activity ◽  
Bovine Embryos ◽  
Akt Signaling Pathway ◽  
Cell Stage ◽  
Development In Vitro

AbstractIn vitro culture can alter the development and quality of bovine embryos. Therefore, we aimed to evaluate whether nobiletin supplementation during EGA improves embryonic development and blastocyst quality and if it affects PI3K/AKT signaling pathway. In vitro zygotes were cultured in SOF + 5% FCS (Control) or supplemented with 5, 10 or 25 µM nobiletin (Nob5, Nob10, Nob25) or with 0.03% dimethyl-sulfoxide (CDMSO) during minor (2 to 8-cell stage; MNEGA) or major (8 to 16-cell stage; MJEGA) EGA phase. Blastocyst yield on Day 8 was higher in Nob5 (42.7 ± 1.0%) and Nob10 (44.4 ± 1.3%) for MNEGA phase and in Nob10 (61.0 ± 0.8%) for MJEGA phase compared to other groups. Mitochondrial activity was higher and lipid content was reduced in blastocysts produced with nobiletin, irrespective of EGA phase. The mRNA abundance of CDK2, H3-3B, H3-3A, GPX1, NFE2L2 and PPARα transcripts was increased in 8-cells, 16-cells and blastocysts from nobiletin groups. Immunofluorescence analysis revealed immunoreactive proteins for p-AKT forms (Thr308 and Ser473) in bovine blastocysts produced with nobiletin. In conclusion, nobiletin supplementation during EGA has a positive effect on preimplantation bovine embryonic development in vitro and corroborates on the quality improvement of the produced blastocysts which could be modulated by the activation of AKT signaling pathway.

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