Mouse homeobox gene transcripts occupy different but overlapping domains in embryonic germ layers and organs: a comparison of Hox-3.1 and Hox-1.5

Development ◽  
1988 ◽  
Vol 103 (1) ◽  
pp. 135-144 ◽  
Author(s):  
S.J. Gaunt
Keyword(s):  
Nervous System ◽  
Homeobox Gene ◽  
Positional Information ◽  
Germ Layers ◽  
Temporal And Spatial Patterns ◽  
Small Delay ◽  
Gene Transcripts ◽  
Overlapping Domains ◽  
Temporal And Spatial

By use of in situ hybridization experiments, the mouse homeobox genes Hox-3.1 and Hox-1.5 are compared in the temporal and spatial patterns of their embryonic transcripts. Transcripts of both genes are first detected at about 7 1/2 days, although the appearance of Hox-3.1 transcripts apparently follows Hox-1.5 after a small delay. Hox-3.1 and Hox-1.5 transcripts occupy domains which are different, although overlapping, along the anteroposterior axis of the embryo. The domains are first established within the ectoderm and mesoderm germ layers at 7 1/2–8 days, but subsequently they persist within the nervous system, the prevertebral column and within at least some of the organs (the thyroid, lung, stomach, mesonephric and metanephric kidneys) at 12 1/2 days. In discussion, two different mechanisms are considered by which positional information might first be generated within the germ layers.

Ultrastructural analysis of unique glycoconjugates in the rat olfactory system

1992 ◽  
Vol 50 (1) ◽  
pp. 890-891
Author(s):  
James E. Crandall ◽  
Linda C. Hassinger ◽  
Gerald A. Schwarting
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Monoclonal Antibodies ◽  
Olfactory System ◽  
Olfactory Bulbs ◽  
Temporal And Spatial Patterns ◽  
Carbohydrate Epitopes ◽  
Olfactory Systems ◽  
Temporal And Spatial ◽  
Cell Surface Glycoconjugates

Cell surface glycoconjugates are considered to play important roles in cell-cell interactions in the developing central nervous system. We have previously described a group of monoclonal antibodies that recognize defined carbohydrate epitopes and reveal unique temporal and spatial patterns of immunoreactivity in the developing main and accessory olfactory systems in rats. Antibody CC2 reacts with complex α-galactosyl and α-fucosyl glycoproteins and glycolipids. Antibody CC1 reacts with terminal N-acetyl galactosamine residues of globoside-like glycolipids. Antibody 1B2 reacts with β-galactosyl glycolipids and glycoproteins. Our light microscopic data suggest that these antigens may be located on the surfaces of axons of the vomeronasal and olfactory nerves as well as on some of their target neurons in the main and accessory olfactory bulbs.

Differential expression of multiple fork head related genes during gastrulation and axial pattern formation in the mouse embryo

Development ◽  
1993 ◽  
Vol 118 (1) ◽  
pp. 47-59 ◽  
Author(s):  
H. Sasaki ◽  
B.L. Hogan
Keyword(s):  
Neural Crest ◽  
Floor Plate ◽  
Definitive Endoderm ◽  
Axis Formation ◽  
Adult Liver ◽  
Temporal And Spatial Patterns ◽  
Fork Head ◽  
Genes Encoding ◽  
Temporal And Spatial

Four genes encoding fork-head-domain-containing proteins (FD genes) have been isolated from a mouse 8.5 days post coitum (p.c.) embryo cDNA library. Two are mouse homologues of rat HNF-3 beta and HNF-3 alpha. The other two are novel and have been named MF-1 and MF-2 (for mesoderm/mesenchyme fork head). Wholemount in situ hybridization of embryos between 6.5 and 9.5 days p. c. shows that each gene has a unique expression pattern. HNF-3 beta is expressed in the node, notochord, floor plate and gut, while HNF-3 alpha is mainly in the definitive endoderm and gut, but also in the floor plate of the midbrain. These results suggest that HNF-3 beta and HNF-3 alpha, in addition to their known functions as transcriptional activators in adult liver, play a role in body axis formation, neural tube patterning and definitive endoderm formation during gastrulation. MF-1 RNA is present in non-notochordal mesoderm, and in neural-crest-derived head mesenchyme, while MF-2 transcripts are found in the sclerotomes of the somites and in head mesenchyme, including that from neural crest. Studies on gastrulation stage embryos suggest that the early temporal and spatial patterns of HNF-3 beta, MF-1 and HNF-3 alpha correlate with populations of cells undergoing commitment to different developmental fates. A model is proposed linking FD gene expression with gastrulation events in the mouse.

Lox10, a member of the NK-2 homeobox gene class, is expressed in a segmental pattern in the endoderm and in the cephalic nervous system of the leech Helobdella

Development ◽  
1993 ◽  
Vol 118 (3) ◽  
pp. 877-892 ◽  
Author(s):  
D. Nardelli-Haefliger ◽  
M. Shankland
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Animal Species ◽  
Homeobox Gene ◽  
Endoderm Differentiation ◽  
Homeodomain Sequence ◽  
Supraesophageal Ganglion ◽  
The Central Nervous System

A novel leech homeobox gene, Lox10, is shown to encode a homeodomain sequence characteristic of a phyletically widespread NK-2 homeobox gene class. Lox10 expression was examined in leech embryos of various ages by in situ hybridization. In the unsegmented cephalic region, Lox10 RNA is expressed in a subset of the cells descended from the a' and b' micromeres, including a small cluster of cells, believed to be postmitotic neurons, within the supraesophageal ganglion of the central nervous system. Hybridization signal was not detected in either the mesoderm or ectoderm of the trunk segments, and the apparent restriction of Lox10 ectodermal expression to the nonsegmented cephalic domain resembles the restricted forebrain expression pattern of its mammalian homologues. Lox10 is also expressed within the endodermal tissues of the leech midgut, which arises by cellularization from a polynucleate syncytium. Endodermal expression is organized into a pattern of transverse stripes and spots which are aligned with the intersegmental septa, and which prefigure the pattern of gut wall constrictions observed at later stages of development. Lox10 is the first molecular marker of segmentally periodic endoderm differentiation reported for any animal species.

scholarly journals Temporal and Spatial Patterns of Enteric Nervous System Regeneration

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Sunny Qi Huang ◽  
Eugenia Cuyar ◽  
Karen Tossas ◽  
Jose E Garcia Arraras
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Spatial Patterns ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial

Detection of Nucleic Acids in Cells or Tissue Sections Using In situ Polymerase Chain Reaction (PCR)

1996 ◽  
Vol 54 ◽  
pp. 16-17
Author(s):  
J. R. Hully ◽  
K. R. Luehrsen ◽  
K. Aoyagi ◽  
C. Shoemaker ◽  
R. Abramson
Keyword(s):  
Nucleic Acids ◽  
Viral Infections ◽  
Anatomical Location ◽  
Rt Pcr ◽  
Reverse Transcription Pcr ◽  
Cellular Source ◽  
Gene Transcripts ◽  
Initial Description ◽  
In Cells

The development of PCR technology has greatly accelerated medical research at the genetic and molecular levels. Until recently, the inherent sensitivity of this technique has been limited to isolated preparations of nucleic acids which lack or at best have limited morphological information. With the obvious exception of cell lines, traditional PCR or reverse transcription-PCR (RT-PCR) cannot identify the cellular source of the amplified product. In contrast, in situ hybridization (ISH) by definition, defines the anatomical location of a gene and/or it’s product. However, this technique lacks the sensitivity of PCR and cannot routinely detect less than 10 to 20 copies per cell. Consequently, the localization of rare transcripts, latent viral infections, foreign or altered genes cannot be identified by this technique. In situ PCR or in situ RT-PCR is a combination of the two techniques, exploiting the sensitivity of PCR and the anatomical definition provided by ISH. Since it’s initial description considerable advances have been made in the application of in situ PCR, improvements in protocols, and the development of hardware dedicated to in situ PCR using conventional microscope slides. Our understanding of the importance of viral latency or viral burden in regards to HIV, HPV, and KSHV infections has benefited from this technique, enabling detection of single viral copies in cells or tissue otherwise thought to be normal. Clearly, this technique will be useful tool in pathobiology especially carcinogenesis, gene therapy and manipulations, the study of rare gene transcripts, and forensics.

Hawk kite as potential bird scare device (the case of pigeons and grain processing factory)

2018 ◽  
Vol 8 (2) ◽  
pp. 334-336
Author(s):  
A. V. Matsyura
Keyword(s):  
Spatial Patterns ◽  
Repellent Effect ◽  
Preliminary Results ◽  
Temporal And Spatial Patterns ◽  
Grain Processing ◽  
Feral Pigeons ◽  
Temporal And Spatial ◽  
Bird Behavior ◽  
Hooded Crows

Here we presented the preliminary results of hawk kite usage against the feral pigeons in some grain processing factory. We studied the temporal and spatial patterns of repellent effect and bird behavior. We suggested the feral pigeons gradually increase the level of tolerance towards the hawk kite if no additional repellent measures were undertaken. Moreover, even initially the feral pigeons demonstrate higher tolerance towards the hawk kite compared to the Rooks or Hooded Crows.

scholarly journals Evolution of Mouse T-box Genes by Tandem Duplication and Cluster Dispersion

Genetics ◽  
1996 ◽  
Vol 144 (1) ◽  
pp. 249-254 ◽  
Author(s):  
Sergei I Agulnik ◽  
Nancy Garvey ◽  
Sarah Hancock ◽  
Ilya Ruvinsky ◽  
Deborah L Chapman ◽  
...  
Keyword(s):  
Tandem Duplication ◽  
Crossing Over ◽  
Dna Binding Domain ◽  
Gene Products ◽  
New Members ◽  
Ancestral Gene ◽  
T Box ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial ◽  
T Locus

Abstract The T-box genes comprise an ancient family of putative transcription factors conserved across species as divergent as Mus musculus and Caenorhabditis elegans. All T-box gene products are characterized by a novel 174-186amino acid DNA binding domain called the T-box that was first discovered in the polypeptide products of the mouse T locus and the Drosophila melanogaster optomotor-blind gene. Earlier studies allowed the identification of five mouse T-box genes, T, Tbx1-3, and Tbr1, that all map to different chromosomal locations and are expressed in unique temporal and spatial patterns during embryogenesis. Here, we report the discovery of three new members of the mouse T-box gene family, named Tbx4, Tbx5, and Tbx6. Two of these newly discovered genes, Tbx4 and Tbx5, were found to be tightly linked to previously identified T-box genes. Combined results from phylogenetic, linkage, and physical mapping studies provide a picture for the evolution of a T-box subfamily by unequal crossing over to form a two-gene cluster that was duplicated and dispersed to two chromosomal locations. This analysis suggests that Tbx4 and Tbx5 are cognate genes that diverged apart from a common ancestral gene during early vertebrate evolution.

scholarly journals Apoptotic Cells, Including Macrophages, Are Prominent in Theiler's Virus-Induced Inflammatory, Demyelinating Lesions

2003 ◽  
Vol 77 (7) ◽  
pp. 4383-4388 ◽  
Author(s):  
Brian P. Schlitt ◽  
Matthew Felrice ◽  
Mary Lou Jelachich ◽  
Howard L. Lipton
Keyword(s):  
Central Nervous System ◽  
T Cells ◽  
Nervous System ◽  
In Situ Hybridization ◽  
Apoptotic Cells ◽  
Theiler's Virus ◽  
Demyelinating Lesions ◽  
Encephalomyelitis Virus ◽  
Mouse Central Nervous System

ABSTRACT Theiler's murine encephalomyelitis virus (TMEV) persists in the mouse central nervous system principally in macrophages, and infected macrophages in culture undergo apoptosis. We have detected abundant apoptotic cells in perivascular cuffs and inflammatory, demyelinating lesions of SJL mice chronically infected with TMEV. T cells comprised 74% of apoptotic cells, while 8% were macrophages, 0.6% were astrocytes, and ∼17% remained unidentified. In situ hybridization revealed viral RNA in ∼1% of apoptotic cells.

Diet of the introduced red fox Vulpes vulpes in Australia: analysis of temporal and spatial patterns

Mammal Review ◽  
2021 ◽  
Author(s):  
Patricia A. Fleming ◽  
Heather M. Crawford ◽  
Alyson M. Stobo‐Wilson ◽  
Stuart J. Dawson ◽  
Christopher R. Dickman ◽  
...  
Keyword(s):  
Spatial Patterns ◽  
Vulpes Vulpes ◽  
Red Fox ◽  
Temporal And Spatial Patterns ◽  
Temporal And Spatial
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