Posterior expression of a homeobox gene in early Xenopus embryos

Development ◽  
1987 ◽  
Vol 101 (1) ◽  
pp. 93-105 ◽  
Author(s):  
B.G. Condie ◽  
R.M. Harland
Keyword(s):  
Amino Acids ◽  
Homeobox Gene ◽  
Specific Expression ◽  
Cell Identity ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Full Length Cdna ◽  
Specific Transcript ◽  
Xenopus Embryos ◽  
Early Embryos

The homeobox containing transcript Xhox-36 is expressed exclusively in the posterior mesoderm and ectoderm of early Xenopus embryos. Therefore, the transcript shows region-specific rather than tissue-specific expression in the gastrula and neurula, a time when cells are becoming committed to defined fates. Exposure of early embryos to LiCl, which shifts posterior cells to more anterior fates, reduces the abundance of this posterior-specific transcript. In contrast, embryos ventralized by u.v. treatment express normal levels of the transcript, implying that expression of the gene is not absolutely linked to dorsal cell identity. The sequence of a full-length cDNA corresponding to this transcript predicts a homeodomain-containing protein of 209 amino acids.

scholarly journals Differential Expression of Caprine β-Defensins in Digestive and Respiratory Tissues

1999 ◽  
Vol 67 (11) ◽  
pp. 6221-6224 ◽  
Author(s):  
Chengquan Zhao ◽  
Tung Nguyen ◽  
Lide Liu ◽  
Olga Shamova ◽  
Kim Brogden ◽  
...  
Keyword(s):  
Amino Acids ◽  
Respiratory Tract ◽  
Differential Expression ◽  
Host Defense ◽  
Specific Expression ◽  
Host Defense Peptides ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Defense Peptides

ABSTRACT We identified two novel β-defensin precursors, preproGBD-1 and preproGBD-2, in the tissues of a goat. Although the precursors were identical in 96.8% of their bases and 88.2% (60 of 68) of their amino acids, preproGBD-1 was expressed principally in the tongue and respiratory tract, whereas preproGBD-2 expression predominated throughout the intestine. These findings exemplify the phenomenon of tissue-specific expression in a family of host defense peptides that arose before the avian and mammalian lineages diverged.

Tissue-specific expression of actin genes injected into Xenopus embryos

Cell ◽  
1986 ◽  
Vol 47 (4) ◽  
pp. 589-599 ◽  
Author(s):  
Clive Wilson ◽  
Gareth S. Cross ◽  
Hugh R. Woodland
Keyword(s):  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression ◽  
Xenopus Embryos ◽  
Actin Genes

scholarly journals Ontogenetic and tissue-specific expression of preproghrelin in the Atlantic halibut, Hippoglossus hippoglossus L.

2007 ◽  
Vol 196 (1) ◽  
pp. 181-192 ◽  
Author(s):  
Anthony J Manning ◽  
Harry M Murray ◽  
Jeffrey W Gallant ◽  
Makoto P Matsuoka ◽  
Emily Radford ◽  
...  
Keyword(s):  
Amino Acids ◽  
Larval Development ◽  
Mouth Opening ◽  
Cell Types ◽  
Atlantic Halibut ◽  
Specific Expression ◽  
Tissue Specific ◽  
Reading Frame ◽  
Tissue Specific Expression ◽  
Different Cell Types

Ghrelin is a conserved vertebrate hormone that affects both GH release and appetite. We have cloned and characterized Atlantic halibut preproghrelin cDNA and examined for the first time preproghrelin expression during fish larval development using quantitative real-time PCR. In addition, cellular sites of expression in larvae and tissue-specific expression in 3-year-old halibut were studied. A full-length cDNA for preproghrelin was isolated from halibut stomach tissue. The 899 bp cDNA encodes an open reading frame of 105 amino acids that is comprised of a signal peptide and two peptides with high similarity to ghrelin and obestatin. The deduced amino acid sequence of halibut ghrelin peptide (GSSFLSPSHKPPKGKPPRA) shows significant conservation relative to other teleostean sequences and is identical to human ghrelin for the first seven amino acids of the sequence. The putative obestatin peptide is well-conserved among fishes but shares limited similarity with its human counterpart. Expression of ghrelin was localized to two different cell types in the stomach of larval halibut by in situ hybridization. However, sensitive PCR assays on tissues collected from 3-year-old fish additionally identified ghrelin transcripts in pyloric caecae, intestine, and in immature ovary and testis. Ontogenetic studies detected ghrelin expression prior to exogenous feeding during larval development (hatching and mouth-opening stages) with increased expression occurring through metamorphosis. This increase was pronounced during climax metamorphosis and coincided with stomach differentiation. Patterns of preproghrelin expression suggest that ghrelin has important roles during and after larval development in halibut, and that ghrelin is associated with digestive and gonadal tissues in this teleost.

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
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