scholarly journals Cell-autonomous FGF signaling regulates anteroposterior patterning and neuronal differentiation in the mesodiencephalic dopaminergic progenitor domain

Development ◽  
2012 ◽  
Vol 139 (5) ◽  
pp. 894-905 ◽  
Author(s):  
L. Lahti ◽  
P. Peltopuro ◽  
T. P. Piepponen ◽  
J. Partanen
Keyword(s):  
Neuronal Differentiation ◽  
Fgf Signaling ◽  
Anteroposterior Patterning ◽  
Progenitor Domain

FGF3 and FGF8 mediate a rhombomere 4 signaling activity in the zebrafish hindbrain

Development ◽  
2002 ◽  
Vol 129 (16) ◽  
pp. 3825-3837 ◽  
Author(s):  
Lisa Maves ◽  
William Jackman ◽  
Charles B. Kimmel
Keyword(s):  
Neuronal Differentiation ◽  
Crucial Role ◽  
Time Lapse ◽  
Signaling Molecules ◽  
Neural Plate ◽  
Fgf Signaling ◽  
Genetic Mosaic ◽  
Signaling Center ◽  
Signaling Activity ◽  
Rhombomere 4

The segmentation of the vertebrate hindbrain into rhombomeres is highly conserved, but how early hindbrain patterning is established is not well understood. We show that rhombomere 4 (r4) functions as an early-differentiating signaling center in the zebrafish hindbrain. Time-lapse analyses of zebrafish hindbrain development show that r4 forms first and hindbrain neuronal differentiation occurs first in r4. Two signaling molecules, FGF3 and FGF8, which are both expressed early in r4, are together required for the development of rhombomeres adjacent to r4, particularly r5 and r6. Transplantation of r4 cells can induce expression of r5/r6 markers, as can misexpression of either FGF3 or FGF8. Genetic mosaic analyses also support a role for FGF signaling acting from r4. Taken together, our findings demonstrate a crucial role for FGF-mediated inter-rhombomere signaling in promoting early hindbrain patterning and underscore the significance of organizing centers in patterning the vertebrate neural plate.

A posteriorising factor, retinoic acid, reveals that anteroposterior patterning controls the timing of neuronal differentiation in Xenopus neuroectoderm

Development ◽  
1996 ◽  
Vol 122 (11) ◽  
pp. 3409-3418 ◽  
Author(s):  
N. Papalopulu ◽  
C. Kintner
Keyword(s):  
Retinoic Acid ◽  
Neuronal Differentiation ◽  
Neural Tissue ◽  
Neural Plate ◽  
Neural Tube Closure ◽  
Proneural Gene ◽  
Anteroposterior Patterning ◽  
Neural Genes ◽  
Tube Closure

During early development of the Xenopus central nervous system (CNS), neuronal differentiation can be detected posteriorly at neural plate stages but is delayed anteriorly until after neural tube closure. A similar delay in neuronal differentiation also occurs in the anterior neural tissue that forms in vitro when isolated ectoderm is treated with the neural inducer noggin. Here we examine the factors that control the timing of neuronal differentiation both in embryos and in neural tissue induced by noggin (noggin caps). We show that the delay in neuronal differentiation that occurs in noggin caps cannot be overcome by inhibiting the activity of the neurogenic gene, X-Delta-1, which normally inhibits neuronal differentiation, suggesting that it represents a novel level of regulation. Conversely, we show that the timing of neuronal differentiation can be changed from late to early after treating noggin caps or embryos with retinoic acid (RA), a putative posteriorising agent. Concommittal with changes in the timing of neuronal differentiation, RA suppresses the expression of anterior neural genes and promotes the expression of posterior neural genes. The level of early neuronal differentiation induced by RA alone is greatly increased by the additional expression of the proneural gene, XASH3. These results indicate that early neuronal differentiation in neuralised ectoderm requires posteriorising signals, as well as signals that promote the activity of proneural genes such as XASH3. In addition, these result suggest that neuronal differentiation is controlled by anteroposterior (A-P) patterning, which exerts a temporal control on the onset of neuronal differentiation.

scholarly journals Fgf-signaling is compartmentalized within the mesenchyme and controls proliferation during salamander limb development

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Sruthi Purushothaman ◽  
Ahmed Elewa ◽  
Ashley W Seifert
Keyword(s):  
Cell Proliferation ◽  
Growth Factor ◽  
Cell Survival ◽  
Fibroblast Growth Factor ◽  
Sonic Hedgehog ◽  
Limb Development ◽  
Fibroblast Growth ◽  
Fgf Signaling ◽  
Anteroposterior Patterning ◽  
Distal Cell

Although decades of studies have produced a generalized model for tetrapod limb development, urodeles deviate from anurans and amniotes in at least two key respects: their limbs exhibit preaxial skeletal differentiation and do not develop an apical ectodermal ridge (AER). Here, we investigated how Sonic hedgehog (Shh) and Fibroblast growth factor (Fgf) signaling regulate limb development in the axolotl. We found that Shh-expressing cells contributed to the most posterior digit, and that inhibiting Shh-signaling inhibited Fgf8 expression, anteroposterior patterning, and distal cell proliferation. In addition to lack of a morphological AER, we found that salamander limbs also lack a molecular AER. We found that amniote and anuran AER-specific Fgfs and their cognate receptors were expressed entirely in the mesenchyme. Broad inhibition of Fgf-signaling demonstrated that this pathway regulates cell proliferation across all three limb axes, in contrast to anurans and amniotes where Fgf-signaling regulates cell survival and proximodistal patterning.

The transcription factor activating protein 2 beta (TFAP2B) mediates neuronal differentiation in neuroblastoma cells

2013 ◽  
Vol 225 (03) ◽  
Author(s):  
F Sherkheli ◽  
S Ackermann ◽  
F Roels ◽  
H Kocak ◽  
R Volland ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Neuronal Differentiation ◽  
Neuroblastoma Cells
Sign in / Sign up

Export Citation Format

Share Document