scholarly journals Interdependency of cell adhesion, force generation and extracellular proteolysis in matrix remodeling

Development ◽  
2011 ◽  
Vol 138 (12) ◽  
pp. e1-e1
Author(s):  
R. Kirmse ◽  
H. Otto ◽  
T. Ludwig
Keyword(s):  
Cell Adhesion ◽  
Adhesion Force ◽  
Force Generation ◽  
Matrix Remodeling ◽  
Extracellular Proteolysis

scholarly journals Evaluation of Cell Adhesion Force with Atomic Force Microscope

2000 ◽  
Vol 40 (supplement) ◽  
pp. S80
Author(s):  
H. Kim ◽  
T. Osada ◽  
A. Ikai
Keyword(s):  
Cell Adhesion ◽  
Atomic Force Microscope ◽  
Adhesion Force ◽  
Atomic Force

scholarly journals The Many Roles of Cell Adhesion Molecules in Hepatic Fibrosis

Cells ◽  
2019 ◽  
Vol 8 (12) ◽  
pp. 1503 ◽  
Author(s):  
Hintermann ◽  
Christen
Keyword(s):  
Cell Adhesion ◽  
Adhesion Molecules ◽  
Hepatic Fibrosis ◽  
Cell Adhesion Molecules ◽  
Tissue Injury ◽  
Immunoglobulin Superfamily ◽  
Cell Contact ◽  
Matrix Remodeling ◽  
Direct Cell ◽  
The Many

Fibrogenesis is a progressive scarring event resulting from disrupted regular wound healing due to repeated tissue injury and can end in organ failure, like in liver cirrhosis. The protagonists in this process, either liver-resident cells or patrolling leukocytes attracted to the site of tissue damage, interact with each other by soluble factors but also by direct cell–cell contact mediated by cell adhesion molecules. Since cell adhesion molecules also support binding to the extracellular matrix, they represent excellent biosensors, which allow cells to modulate their behavior based on changes in the surrounding microenvironment. In this review, we focus on selectins, cadherins, integrins and members of the immunoglobulin superfamily of adhesion molecules as well as some non-classical cell adhesion molecules in the context of hepatic fibrosis. We describe their liver-specific contributions to leukocyte recruitment, cell differentiation and survival, matrix remodeling or angiogenesis and touch on their suitability as targets in antifibrotic therapies.

scholarly journals Evaluation of the Correlation between Focal Adhesion Kinase Phosphorylation and Cell Adhesion Force Using “DEP” Technology

Sensors ◽  
2012 ◽  
Vol 12 (5) ◽  
pp. 5951-5965 ◽  
Author(s):  
Chyung Ay ◽  
Chih-Chang Yeh ◽  
Min-Chih Hsu ◽  
Huaang-Youh Hurng ◽  
Philip Chi Lip Kwok ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Focal Adhesion Kinase ◽  
Focal Adhesion ◽  
Adhesion Force ◽  
Focal Adhesion Kinase Phosphorylation ◽  
Kinase Phosphorylation

scholarly journals Aberrant DNA Methylation of Matrix Remodeling and Cell Adhesion Related Genes in Pterygium

PLoS ONE ◽  
2011 ◽  
Vol 6 (2) ◽  
pp. e14687 ◽  
Author(s):  
Andri K. Riau ◽  
Tina T. Wong ◽  
Sharon N. Finger ◽  
Shyam S. Chaurasia ◽  
Ai Hua Hou ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cell Adhesion ◽  
Matrix Remodeling ◽  
Aberrant Dna Methylation

Emergence of Multicellular Organisms with Dynamic Differentiation and Spatial Pattern

1998 ◽  
Vol 4 (1) ◽  
pp. 79-93 ◽  
Author(s):  
Chikara Furusawa ◽  
Kunihiko Kaneko
Keyword(s):  
Cell Adhesion ◽  
Spatial Pattern ◽  
Spatial Information ◽  
Adhesion Force ◽  
Cell Interaction ◽  
Dynamical Instability ◽  
Differentiated Cells ◽  
State Dependent ◽  
Multicellular Organisms ◽  
Cell Cell

The origin of multicellular organisms and the mechanism of development in cell societies are studied by choosing a model with intracellular biochemical dynamics allowing for oscillations, cell–cell interaction through diffusive chemicals on a two-dimensional grid, and state-dependent cell adhesion. Cells differentiate due to a dynamical instability, as described by our “isologous diversification” theory. A fixed spatial pattern of differentiated cells emerges, where spatial information is sustained by cell–cell interactions. This pattern is robust against perturbations. With an adequate cell adhesion force, active cells are released that form the seed of a new generation of multicellular organisms, accompanied by death of the original multicellular unit as a halting state. It is shown that the emergence of multicellular organisms with differentiation, regulation, and life cycle is not an accidental event, but a natural consequence in a system of replicating cells with growth.

scholarly journals Predicting metastasis with a novel biophysical cell-adhesion force technique

2020 ◽  
Author(s):  
Jessie Gan ◽  
Zhao Zhihai ◽  
Yu Miao
Keyword(s):  
Cell Adhesion ◽  
Roc Curve ◽  
Adhesion Force ◽  
Metastatic Potential ◽  
The Novel ◽  
Adhesion Forces ◽  
Potential Force ◽  
Or Efficiency ◽  
Nih3t3 Cells ◽  
Metastatic Cells

AbstractMetastasis is widely accepted to be responsible for approximately 90% of all cancer deaths. Current research on metastasis prediction often centers on gene sequencing; however, these analyses must account for the complexity of gene regulation and rely on comprehensive datasets. To investigate the process from a simpler, non-genomic angle, some studies indicate differences in cell adhesion force, an important physical process in metastasizing cells. However, cell adhesion force methods tend to focus on cell population approaches and therefore have their drawbacks in cost or efficiency, rendering them impractical outside a research setting. In this work, we test a novel and inexpensive bead-pipette assay to investigate the adhesion forces of non-metastatic NIH3T3 cells and mutated RasV12 cells, a metastatic model cell line.Control cells and RasV12 cells were evaluated with wound healing, spreading area, and focal adhesion (FA) analysis assays. Then cells were tested by the novel bead-pipette assay, which uses a fibronectin-coated bead and a glass micropipette to measure cell adhesion force using Hooke’s law.The RasV12 cells had faster migration, polarized cell shape, and smaller FA area than control cells. The RasV12 cells also exerted higher adhesion forces than control cells and a potential force threshold was determined for distinguishing metastatic cells through a Receiver Operating Characteristic (ROC) curve. An ROC curve was computed for all other assays and the bead-pipette assay was shown to perform higher as a classifier than other assays.The RasV12 cells had increased metastatic potential compared to control. The novel bead-pipette assay showed potential as a classifier for determining metastasizing cells from non-metastatic cells. With further work, it may serve as a clinical diagnostic tool for cancer patients or as a testbed to be used in the development of anti-metastatic drugs.

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