scholarly journals Msx genes define a population of mural cell precursors required for head blood vessel maturation

Development ◽  
2011 ◽  
Vol 138 (14) ◽  
pp. 3055-3066 ◽  
Author(s):  
M. Lopes ◽  
O. Goupille ◽  
C. S. Cloment ◽  
Y. Lallemand ◽  
A. Cumano ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Mural Cell ◽  
Vessel Maturation ◽  
Msx Genes

The Notch1-Dll4 signaling pathway regulates mouse postnatal lymphatic development

Blood ◽  
2011 ◽  
Vol 118 (7) ◽  
pp. 1989-1997 ◽  
Author(s):  
Kyle Niessen ◽  
Gu Zhang ◽  
John Brady Ridgway ◽  
Hao Chen ◽  
Ganesh Kolumam ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Notch Signaling ◽  
Signaling Pathway ◽  
Lymphatic Vessels ◽  
Mouse Skin ◽  
Notch Signaling Pathway ◽  
Cell Phenotype ◽  
Mural Cell ◽  
Lymphatic Development ◽  
Vessel Development

Abstract The Notch signaling pathway plays a fundamental role during blood vessel development. Notch signaling regulates blood vessel morphogenesis by promoting arterial endothelial differentiation and pro-viding spatial and temporal control over “tip cell” phenotype during angiogenic sprouting. Components of the Notch signaling pathway have emerged as potential regulators of lymphatic development, joining the increasing examples of blood vessel regulators that are also involved in lymphatic development. However, in mammals a role for the Notch signaling pathway during lymphatic development remains to be demonstrated. In this report, we show that blockade of Notch1 and Dll4, with specific function-blocking antibodies, results in defective postnatal lymphatic development in mice. Mechanistically, Notch1-Dll4 blockade is associated with down-regulation of EphrinB2 expression, been shown to be critically involved in VEGFR3/VEGFC signaling, resulting in reduced lymphangiogenic sprouting. In addition, Notch1-Dll4 blockade leads to compromised expression of distinct lymphatic markers and to dilation of collecting lymphatic vessels with reduced and disorganized mural cell coverage. Finally, Dll4-blockade impairs wound closure and severely affects lymphangiogenesis during the wound healing in adult mouse skin. Thus, our study demonstrates for the first time in a mammalian system that Notch1-Dll4 signaling pathway regulates postnatal lymphatic development and pathologic lymphangiogenesis.

Neuropilin regulation of angiogenesis

2014 ◽  
Vol 42 (6) ◽  
pp. 1623-1628 ◽  
Author(s):  
Anastasia Lampropoulou ◽  
Christiana Ruhrberg
Keyword(s):  
Blood Vessel ◽  
Vascular Permeability ◽  
Current Knowledge ◽  
Transmembrane Protein ◽  
Vascular Pathology ◽  
Eye Disease ◽  
Vegf Receptor ◽  
Neuropilin 1 ◽  
Vessel Growth ◽  
Vessel Maturation

Blood vessel formation during vertebrate development relies on a process called angiogenesis and is essential for organ growth and tissue viability. In addition, angiogenesis leads to pathological blood vessel growth in diseases with tissue ischaemia, such as neovascular eye disease and cancer. Neuropilin 1 (NRP1) is a transmembrane protein that serves as a receptor for the VEGF165 isoform of the vascular endothelial growth factor (VEGF) to enhance cell migration during angiogenesis via VEGF receptor 2 (VEGFR2), and it is also essential for VEGF-induced vascular permeability and arteriogenesis. In addition, NRP1 activation affects angiogenesis independently of VEGF signalling by activating the intracellular kinase ABL1. NRP1 also acts as a receptor for the class 3 semaphorin (SEMA3A) to regulate vessel maturation during tumour angiogenesis and vascular permeability in eye disease. In the present paper, we review current knowledge of NRP1 regulation during angiogenesis and vascular pathology.

Abstract TP475: Effect of Udenafil, a Phosphodiesterase-5 Inhibitor, on Angiogenesis in a Cav-1 Deficient Moyamoya Disease Model

Stroke ◽  
2020 ◽  
Vol 51 (Suppl_1) ◽  
Author(s):  
Hyung Jun Kim ◽  
Oh Young Bang ◽  
In-Young Baek ◽  
Jae-Hwan Kim ◽  
Ye Sel Kim ◽  
...  
Keyword(s):  
Moyamoya Disease ◽  
Growth Factor Receptor ◽  
Guanosine Monophosphate ◽  
Therapeutic Effects ◽  
Mural Cell ◽  
Phosphodiesterase 5 Inhibitor ◽  
Vessel Maturation ◽  
Phosphodiesterase 5

Background: Although pathogenic mechanisms of moyamoya disease remain unknown, recent studies suggest that it is a caveolae disease. This study evaluated the effect of udenafil, a phosphodiesterase-5 inhibitor, on vessel maturation in in vitro and in vivo moyamoya disease models. Methods: Angiogenesis and vessel maturation were assessed in in vitro models, caveolin-1 (Cav-1) knockdown human umbilical vessel endothelial cells (HUVECs) and coronary artery smooth muscle cells (CASMCs), and in in vivo model of bilateral internal carotid artery occlusion (bICAo). Udenafil was administered (1,3,10 and 30 μM) in cell culture conditions, and functional studies (migration and tube formation assay) were performed and vessel maturation factors and cyclic guanosine monophosphate (cGMP) accumulation were measured. Udenafil (3 and 10 mg/kg) was orally administered once daily for 4 weeks in bICAo rat model, and histological analysis for angiogenesis and vessel maturation was performed. Results: Udenafil increased vessel formation in both Cav-1 knockdown HUVEC and bICAo models without increased migration/proliferation of HUVECs and CASMCs. Udenafil increased CD31+ vessel density and NG2/Col4+ mural cell density in bICAo models. Cav-1 knockdown inhibited accumulation of cGMP, and udenafil treatment restored cGMP levels in Cav-1 knockdown HUVEC models. Vessel maturation factors (angiopoietin-1 and platelet-derived growth factor receptor-β) and angiogenic factors (endothelial nitric oxide synthase) were increased after treatment with udenafil in vitro . Conclusion: Our results indicate that udenafil reversed cellular levels of cGMP related to Cav-1 deficiency and induced angiogenesis and vessel maturation. Further studies are warranted to confirm the therapeutic effects of this strategy in moyamoya disease.

scholarly journals Changes in blood vessel maturation in the fibrous cap of the tumor rim

2011 ◽  
Vol 103 (3) ◽  
pp. 433-438 ◽  
Author(s):  
Hisamichi Naito ◽  
Kazuhiro Takara ◽  
Taku Wakabayashi ◽  
Hiroki Kawahara ◽  
Hiroyasu Kidoya ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Fibrous Cap ◽  
Vessel Maturation

Loss of smooth muscle calponin results in impaired blood vessel maturation in the tumor?host microenvironment

2007 ◽  
Vol 98 (5) ◽  
pp. 757-763 ◽  
Author(s):  
Hisako Yamamura ◽  
Noriko Hirano ◽  
Hidenori Koyama ◽  
Yoshiki Nishizawa ◽  
Katsuhito Takahashi
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Vessel Maturation
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