scholarly journals Sonic hedgehog-dependent synthesis of laminin  1 controls basement membrane assembly in the myotome

Development ◽  
2009 ◽  
Vol 136 (20) ◽  
pp. 3495-3504 ◽  
Author(s):  
C. Anderson ◽  
S. Thorsteinsdottir ◽  
A.-G. Borycki
Keyword(s):  
Basement Membrane ◽  
Sonic Hedgehog ◽  
Membrane Assembly ◽  
Laminin 1

scholarly journals Inhibition of laminin alpha 1-chain expression leads to alteration of basement membrane assembly and cell differentiation.

1996 ◽  
Vol 133 (2) ◽  
pp. 417-430 ◽  
Author(s):  
A De Arcangelis ◽  
P Neuville ◽  
R Boukamel ◽  
O Lefebvre ◽  
M Kedinger ◽  
...  
Keyword(s):  
Cell Differentiation ◽  
Basement Membrane ◽  
Regulatory Element ◽  
Digestive Enzyme ◽  
Eukaryotic Expression ◽  
Basement Membranes ◽  
Chain Gene ◽  
Membrane Assembly ◽  
Development And Differentiation ◽  
Laminin 1

The expression of the constituent alpha 1 chain of laminin-1, a major component of basement membranes, is markedly regulated during development and differentiation. We have designed an antisense RNA strategy to analyze the direct involvement of the alpha 1 chain in laminin assembly, basement membrane formation, and cell differentiation. We report that the absence of alpha 1-chain expression, resulting from the stable transfection of the human colonic cancer Caco2 cells with an eukaryotic expression vector comprising a cDNA fragment of the alpha 1 chain inserted in an antisense orientation, led to (a) an incorrect secretion of the two other constituent chains of laminin-1, the beta 1/gamma 1 chains, (b) the lack of basement membrane assembly when Caco2-deficient cells were cultured on top of fibroblasts, assessed by the absence of collagen IV and nidogen deposition, and (c) changes in the structural polarity of cells accompanied by the inhibition of an apical digestive enzyme, sucrase-isomaltase. The results demonstrate that the alpha 1 chain is required for secretion of laminin-1 and for the assembly of basement membrane network. Furthermore, expression of the laminin alpha 1-chain gene may be a regulatory element in determining cell differentiation.

scholarly journals Laminin  1 globular domains 4-5 induce fetal development but are not vital for embryonic basement membrane assembly

2005 ◽  
Vol 102 (5) ◽  
pp. 1502-1506 ◽  
Author(s):  
S. Scheele ◽  
M. Falk ◽  
A. Franzen ◽  
F. Ellin ◽  
M. Ferletta ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Fetal Development ◽  
Membrane Assembly ◽  
Laminin 1

scholarly journals Laminin

2004 ◽  
Vol 164 (7) ◽  
pp. 959-963 ◽  
Author(s):  
Takako Sasaki ◽  
Reinhard Fässler ◽  
Erhard Hohenester
Keyword(s):  
Basement Membrane ◽  
Membrane Assembly ◽  
Structural Methods ◽  
Laminin 1

Laminin-1 is emerging as the key molecule in early embryonic basement membrane assembly. Here we review recent insights into its functions gained from the synergistic application of genetic and structural methods.

Deficiency of β1 Integrins in Teratoma Interferes with Basement Membrane Assembly and Laminin-1 Expression

1998 ◽  
Vol 238 (1) ◽  
pp. 70-81 ◽  
Author(s):  
Takako Sasaki ◽  
Erik Forsberg ◽  
Wilhelm Bloch ◽  
Klaus Addicks ◽  
Reinhard Fässler ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Membrane Assembly ◽  
Β1 Integrins ◽  
Laminin 1

scholarly journals 13-P008 Sonic hedgehog-dependent control of basement membrane assembly in the myotome

2009 ◽  
Vol 126 ◽  
pp. S196-S197
Author(s):  
Claire Anderson ◽  
Solveig Thorsteinsdottir ◽  
Anne-Gaëlle Borycki
Keyword(s):  
Basement Membrane ◽  
Sonic Hedgehog ◽  
Membrane Assembly

Basement membrane assembly, stability and activities observed through a developmental lens

2004 ◽  
Vol 22 (7) ◽  
pp. 521-538 ◽  
Author(s):  
Peter D. Yurchenco ◽  
Peter S. Amenta ◽  
Bruce L. Patton
Keyword(s):  
Basement Membrane ◽  
Membrane Assembly

scholarly journals Role of Laminin Terminal Globular Domains in Basement Membrane Assembly

2007 ◽  
Vol 282 (29) ◽  
pp. 21437-21447 ◽  
Author(s):  
Karen K. McKee ◽  
David Harrison ◽  
Stephanie Capizzi ◽  
Peter D. Yurchenco
Keyword(s):  
Basement Membrane ◽  
Membrane Assembly

"BM180": a novel basement membrane protein with a role in stimulus-secretion coupling by lacrimal acinar cells

1996 ◽  
Vol 270 (6) ◽  
pp. C1743-C1750 ◽  
Author(s):  
G. W. Laurie ◽  
J. D. Glass ◽  
R. A. Ogle ◽  
C. M. Stone ◽  
J. R. Sluss ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Membrane Protein ◽  
Acinar Cells ◽  
Basement Membranes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Regulated Secretion ◽  
Basement Membrane Protein ◽  
Stimulus Secretion Coupling ◽  
Lacrimal Acinar Cells ◽  
Laminin 1

Regulated secretion requires the developmental coupling of neuronal or hormonal stimuli to an exocytotic response, a multistep pathway whose appearance may be linked with cellular adhesion to the newly formed exocrine cell basement membrane. We screened for adhesion-associated coupling activity using lacrimal acinar cells and have identified “BM180”, a novel basement membrane protein enriched in guanidine HCl extracts of lacrimal and parotid exocrine secretory glands. BM180 resides primarily in a previously inexamined lower molecular-mass basement membrane peak (peak 2) that contains cell adhesion activity inhibitable with the anti-BM180 monoclonal antibody 3E12. Removal of peak 2 by gel filtration or preincubation of basement membrane with 3E12 decreased regulated peroxidase secretion by one-half without affecting constitutive secretion or the amount of cellular peroxidase available for release. Adding back peak 2 restored regulated secretion in a dose-dependent and 3E12-inhibitable manner and suggested a synergistic relationship between BM180 and laminin 1. BM180 has a mobility of 180 and 60 kDa in the absence or presence of dithiothreitol, respectively, and shows no immunological identity by competitive enzyme-linked immunosorbent assay with laminin 1, collagen IV, entactin, fibronectin, BM-40, perlecan, or vitronectin. We propose that BM180 is an important resident of certain glandular basement membranes where it interacts with the cell surface, thereby possibly signaling the appearance of a transducing element in the stimulus-secretion coupling pathway.

scholarly journals Matrix assembly, regulation, and survival functions of laminin and its receptors in embryonic stem cell differentiation

2002 ◽  
Vol 157 (7) ◽  
pp. 1279-1290 ◽  
Author(s):  
Shaohua Li ◽  
David Harrison ◽  
Salvatore Carbonetto ◽  
Reinhard Fässler ◽  
Neil Smyth ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Cell Mass ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Embryoid Bodies ◽  
Embryonic Stem Cell Differentiation ◽  
Companion Matrix ◽  
Β1 Integrin ◽  
Matrix Assembly ◽  
Membrane Assembly

Laminin-1 is essential for early embryonic basement membrane assembly and differentiation. Several steps can be distinguished, i.e., the expression of laminin and companion matrix components, their accumulation on the cell surface and assembly into basement membrane between endoderm and inner cell mass, and the ensuing differentiation of epiblast. In this study, we used differentiating embryoid bodies derived from mouse embryonic stem cells null for γ1-laminin, β1-integrin and α/β-dystroglycan to dissect the contributions of laminin domains and interacting receptors to this process. We found that (a) laminin enables β1-integrin–null embryoid bodies to assemble basement membrane and achieve epiblast with β1-integrin enabling expression of the laminin α1 subunit; (b) basement membrane assembly and differentiation require laminin polymerization in conjunction with cell anchorage, the latter critically dependent upon a heparin-binding locus within LG module-4; (c) dystroglycan is not uniquely required for basement membrane assembly or initial differentiation; (d) dystroglycan and integrin cooperate to sustain survival of the epiblast and regulate laminin expression; and (e) laminin, acting via β1-integrin through LG1–3 and requiring polymerization, can regulate dystroglycan expression.

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