scholarly journals The YPWM motif links Antennapedia to the basal transcriptional machinery

Development ◽  
2008 ◽  
Vol 135 (9) ◽  
pp. 1669-1679 ◽  
Author(s):  
F. Prince ◽  
T. Katsuyama ◽  
Y. Oshima ◽  
S. Plaza ◽  
D. Resendez-Perez ◽  
...  
Keyword(s):  
Basal Transcriptional Machinery ◽  
Transcriptional Machinery

scholarly journals Interaction and functional collaboration of p300 and C/EBPbeta.

1997 ◽  
Vol 17 (11) ◽  
pp. 6609-6617 ◽  
Author(s):  
S Mink ◽  
B Haenig ◽  
K H Klempnauer
Keyword(s):  
Binding Site ◽  
Protein Interactions ◽  
Ectopic Expression ◽  
Dominant Negative ◽  
Creb Binding Protein ◽  
Adenovirus E1a ◽  
Basal Transcriptional Machinery ◽  
P300 Binding ◽  
Transcriptional Machinery ◽  
Interaction Map

Transcriptional coactivators such as p300 and CREB-binding protein (CBP) function as important elements in the transcription factor network, linking individual transactivators via protein-protein interactions to the basal transcriptional machinery. We have investigated whether p300 plays a role in transactivation mediated by C/EBPbeta, a conserved member of the C/EBP family. We show that C/EBPbeta-dependent transactivation is strongly inhibited by adenovirus E1A but not by E1A mutants defective in p300 binding. Ectopic expression of p300 reverses the E1A-dependent inhibition and increases the transactivation potential of C/EBPbeta. Furthermore, we show that C/EBPbeta and p300 interact with each other and demonstrate that the sequences responsible for interaction map to the E1A binding region of p300 and the amino terminus of C/EBPbeta. Finally, we show that the minimal C/EBPbeta binding site of p300 acts as a dominant-negative inhibitor of C/EBPbeta. These observations identify p300 as a bona fide coactivator for C/EBPbeta. C/EBPbeta is highly expressed in the myelomonocytic lineage of the hematopoietic system and cooperates with Myb to activate mim-1, a gene specifically expressed during myelomonocytic differentiation. Recent evidence has shown that Myb recruits CBP (and presumably p300) as a coactivator and, in contrast to C/EBPbeta, interacts with the CREB binding site of p300-CBP. We show that p300 not only stimulates the activity of Myb and C/EBPbeta individually but also increases the synergy between them. Thus, our results reveal a novel function of p300: in addition to linking specific transcription factors to the basal transcriptional machinery, p300 also mediates the cooperation between transactivators interacting with different domains of p300.

Caenorhabditis elegans unc-37/groucho Interacts Genetically With Components of the Transcriptional Mediator Complex

Genetics ◽  
2002 ◽  
Vol 160 (2) ◽  
pp. 799-803
Author(s):  
Hong Zhang ◽  
Scott W Emmons
Keyword(s):  
Caenorhabditis Elegans ◽  
Sensory Neurons ◽  
Chromatin Structure ◽  
Synthetic Lethality ◽  
Mediator Complex ◽  
Developmental Processes ◽  
Basal Transcriptional Machinery ◽  
Transcriptional Machinery

Abstract Groucho functions as a general corepressor by modulating chromatin structure and has a widespread role in many developmental processes. Here we show that Groucho may also interact with the basal transcriptional machinery. Mutations in Caenorhabditis elegans groucho interact with mutations in components of the transcriptional Mediator complex, resulting in synthetic lethality and loss of male sensory neurons.

scholarly journals Responses of Four Yeast Genes to Changes in the Transcriptional Machinery Are Determined by Their Promoters

2002 ◽  
Vol 12 (21) ◽  
pp. 1828-1832 ◽  
Author(s):  
Jason X Cheng ◽  
Monique Floer ◽  
Paul Ononaji ◽  
Gene Bryant ◽  
Mark Ptashne
Keyword(s):  
Transcriptional Machinery ◽  
Yeast Genes

scholarly journals Elements of Transcriptional Machinery Are Compatible among Plants and Mammals

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e53737 ◽  
Author(s):  
Annette Wolf ◽  
Nina Akrap ◽  
Berenice Marg ◽  
Helena Galliardt ◽  
Martyna Heiligentag ◽  
...  
Keyword(s):  
Transcriptional Machinery
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