scholarly journals Fat facets and Liquid facets promote Delta endocytosis and Delta signaling in the signaling cells

Development ◽  
2004 ◽  
Vol 131 (21) ◽  
pp. 5355-5366 ◽  
Author(s):  
E. Overstreet
Keyword(s):  
Fat Facets ◽  
Liquid Facets

scholarly journals The function of the Drosophila fat facets deubiquitinating enzyme in limiting photoreceptor cell number is intimately associated with endocytosis

Development ◽  
2000 ◽  
Vol 127 (8) ◽  
pp. 1727-1736 ◽  
Author(s):  
A.L. Cadavid ◽  
A. Ginzel ◽  
J.A. Fischer
Keyword(s):  
Compound Eye ◽  
Cell Communication ◽  
Photoreceptor Cells ◽  
Cell Number ◽  
Deubiquitinating Enzyme ◽  
A Cell ◽  
Fat Facets ◽  
Liquid Facets ◽  
And Function ◽  
Communication Pathway

Fat facets is a deubiquitinating enzyme required in a cell communication pathway that limits to eight the number of photoreceptor cells in each facet of the Drososphila compound eye. Genetic data support a model whereby Faf removes ubiquitin, a polypeptide tag for protein degradation, from a specific ubiquitinated protein thus preventing its degradation. Here, mutations in the liquid facets gene were identified as dominant enhancers of the fat facets mutant eye phenotype. The liquid facets locus encodes epsin, a vertebrate protein associated with the clathrin endocytosis complex. The results of genetic experiments reveal that fat facets and liquid facets facilitate endocytosis and function in common cells to generate an inhibitory signal that prevents ectopic photoreceptor determination. Moreover, it is demonstrated that the fat facets mutant phenotype is extraordinarily sensitive to the level of liquid facets expression. We propose that Liquid facets is a candidate for the critical substrate of Fat facets in the eye.

scholarly journals The Ras Target AF-6 is a Substrate of the Fam Deubiquitinating Enzyme

1998 ◽  
Vol 142 (4) ◽  
pp. 1053-1062 ◽  
Author(s):  
Shinichiro Taya ◽  
Takaharu Yamamoto ◽  
Kyoko Kano ◽  
Yoji Kawano ◽  
Akihiro Iwamatsu ◽  
...  
Keyword(s):  
Cell Fate ◽  
Plasma Membranes ◽  
Critical Role ◽  
Amino Acid Sequences ◽  
Cell Contact ◽  
Deubiquitinating Enzyme ◽  
Intact Cells ◽  
Cell Adhesions ◽  
Fat Facets ◽  
Cell Cell

The Ras target AF-6 has been shown to serve as one of the peripheral components of cell–cell adhesions, and is thought to participate in cell–cell adhesion regulation downstream of Ras. We here purified an AF-6-interacting protein with a molecular mass of ∼220 kD (p220) to investigate the function of AF-6 at cell–cell adhesions. The peptide sequences of p220 were identical to the amino acid sequences of mouse Fam. Fam is homologous to a deubiquitinating enzyme in Drosophila, the product of the fat facets gene. Recent genetic analyses indicate that the deubiquitinating activity of the fat facets product plays a critical role in controlling the cell fate. We found that Fam accumulated at the cell–cell contact sites of MDCKII cells, but not at free ends of plasma membranes. Fam was partially colocalized with AF-6 and interacted with AF-6 in vivo and in vitro. We also showed that AF-6 was ubiquitinated in intact cells, and that Fam prevented the ubiquitination of AF-6.

RETRACTED: Stage- and sex-dependent expressions of Usp9x, an X-linked mouse ortholog of Drosophila Fat facets, during gonadal development and oogenesis in mice

2002 ◽  
Vol 2 (1-2) ◽  
pp. 87-91 ◽  
Author(s):  
Takashi Noma ◽  
Yoshiakira Kanai ◽  
Masami Kanai-Azuma ◽  
Maki Ishii ◽  
Masahiko Fujisawa ◽  
...  
Keyword(s):  
Gonadal Development ◽  
Fat Facets

Stage- and sex-dependent expressions of Usp9x, an X-linked mouse ortholog of Drosophila Fat facets, during gonadal development and oogenesis in mice

2002 ◽  
Vol 119 ◽  
pp. S91-S95 ◽  
Author(s):  
Takashi Noma ◽  
Yoshiakira Kanai ◽  
Masami Kanai-Azuma ◽  
Maki Ishii ◽  
Masahiko Fujisawa ◽  
...  
Keyword(s):  
Gonadal Development ◽  
Fat Facets

Fat facets does a Highwire act at the synapse

BioEssays ◽  
2002 ◽  
Vol 24 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Janice A. Fischer ◽  
Erin Overstreet
Keyword(s):  
Fat Facets

scholarly journals Identification of Genes That Interact With Drosophila liquid facets

Genetics ◽  
2006 ◽  
Vol 175 (3) ◽  
pp. 1163-1174 ◽  
Author(s):  
Suk Ho Eun ◽  
Kristi Lea ◽  
Erin Overstreet ◽  
Samuel Stevens ◽  
Ji-Hoon Lee ◽  
...  
Keyword(s):  
Liquid Facets

Control of Cell Fate by a Deubiquitinating Enzyme Encoded by the fat facets Gene

Science ◽  
1995 ◽  
Vol 270 (5243) ◽  
pp. 1828-1831 ◽  
Author(s):  
Y. Huang ◽  
R. T. Baker ◽  
J. A. Fischer-Vize
Keyword(s):  
Cell Fate ◽  
Deubiquitinating Enzyme ◽  
Fat Facets

scholarly journals Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice

Reproduction ◽  
2017 ◽  
Vol 154 (2) ◽  
pp. 135-143 ◽  
Author(s):  
Kasane Kishi ◽  
Aya Uchida ◽  
Hinako M Takase ◽  
Hitomi Suzuki ◽  
Masamichi Kurohmaru ◽  
...  
Keyword(s):  
Cell Death ◽  
Germ Cell ◽  
Apoptotic Cell ◽  
Cell Lineage ◽  
Conditional Knockout ◽  
Spermatogenic Cells ◽  
Male Mice ◽  
Conditional Deletion ◽  
Fat Facets

USP9X (ubiquitin-specific peptidase 9, X chromosome) is the mammalian orthologue of Drosophila deubiquitinase fat facets that was previously shown to regulate the maintenance of the germ cell lineage partially through stabilizing Vasa, one of the widely conserved factors crucial for gametogenesis. Here, we demonstrate that USP9X is expressed in the gonocytes and spermatogonia in mouse testes from newborn to adult stages. By using Vasa-Cre mice, germ cell-specific conditional deletion of Usp9x from the embryonic stage showed no abnormality in the developing testes by 1 week and no appreciable defects in the undifferentiated and differentiating spermatogonia at postnatal and adult stages. Interestingly, after 2 weeks, Usp9x-null spermatogenic cells underwent apoptotic cell death at the early spermatocyte stage, and then, caused subsequent aberrant spermiogenesis, which resulted in a complete infertility of Usp9x conditional knockout male mice. These data provide the first evidence of the crucial role of the spermatogonial USP9X during transition from the mitotic to meiotic phases and/or maintenance of early meiotic phase in Usp9x conditional knockout testes.

scholarly journals Genetic Analysis of the DrosophilaDNAprimGene: The Function of the 60-kD Primase Subunit of DNA Polymerase Opposes thefat facetsSignaling Pathway in the Developing Eye

Genetics ◽  
2000 ◽  
Vol 156 (4) ◽  
pp. 1787-1795 ◽  
Author(s):  
Xin Chen ◽  
Qinghong Li ◽  
Janice A Fischer
Keyword(s):  
Dna Polymerase ◽  
Dna Sequences ◽  
Compound Eye ◽  
Large Subunit ◽  
Cell Communication ◽  
Dna Primase ◽  
Morphological Defects ◽  
Dependent Cell ◽  
Fat Facets ◽  
Communication Pathway

AbstractThe Drosophila DNAprim gene encodes the large subunit (60 kD) of DNA primase, the part of DNA polymerase α that synthesizes RNA primers during DNA replication. The precise function of the 60-kD subunit is unknown. In a mutagenesis screen for suppressors of the fat facets (faf) mutant eye phenotype, we identified mutations in DNAprim. The faf gene encodes a deubiquitinating enzyme required specifically for patterning the compound eye. The DNA sequences of four DNAprim alleles were determined and these define essential protein domains. We show that while flies lacking DNAprim activity are lethal, flies with reduced DNAprim activity display morphological defects in their eyes, and unlike faf mutants, cell cycle abnormalities in larval eye discs. Mechanisms by which DNA primase levels might influence the faf-dependent cell communication pathway are discussed.

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