scholarly journals Molecular profiling of stem cell-like female germ line cells in Drosophila delineates networks important for stemness and differentiation

Biology Open ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. bio046789 ◽  
Author(s):  
Manu D. Tiwari ◽  
Daniela M. Zeitler ◽  
Gunter Meister ◽  
Andreas Wodarz
Keyword(s):  
Stem Cell ◽  
Germ Line ◽  
Molecular Profiling ◽  
Female Germ Line

scholarly journals Isolation and Nature of Mouse Embryonic Stem Cell Line Efficiently Producing Female Germ-line Chimeras

1995 ◽  
Vol 66 (4) ◽  
pp. 361-367
Author(s):  
Masaki UCHIDA ◽  
Tomoyuki TOKUNAGA ◽  
Shinichi AIZAWA ◽  
Koji NIWA ◽  
Hiroshi IMAI
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Cell Line ◽  
Germ Line ◽  
Embryonic Stem Cell Line ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Line ◽  
Female Germ Line

scholarly journals spenito is required for sex determination in Drosophila melanogaster

2015 ◽  
Vol 112 (37) ◽  
pp. 11606-11611 ◽  
Author(s):  
Dong Yan ◽  
Norbert Perrimon
Keyword(s):  
Drosophila Melanogaster ◽  
Stem Cell ◽  
Alternative Splicing ◽  
Sex Determination ◽  
Nuclear Protein ◽  
Germ Line ◽  
Splicing Regulation ◽  
Protein Loss ◽  
Family Protein ◽  
Female Germ Line

Sex-lethal (Sxl) encodes the master regulator of the sex determination pathway in Drosophila and acts by controlling sex identity in both soma and germ line. In females Sxl maintains its own expression by controlling the alternative splicing of its own mRNA. Here, we identify a novel sex determination gene, spenito (nito) that encodes a SPEN family protein. Loss of nito activity results in stem cell tumors in the female germ line as well as female-to-male somatic transformations. We show that Nito is a ubiquitous nuclear protein that controls the alternative splicing of the Sxl mRNA by interacting with Sxl protein and pre-mRNA, suggesting that it is directly involved in Sxl auto-regulation. Given that SPEN family proteins are frequently mutated in cancers, our results suggest that these factors might be implicated in tumorigenesis through splicing regulation.

pitkinD, a Novel Gain-of-Function Enhancer of Position-Effect Variegation, Affects Chromatin Regulation During Oogenesis and Early Embryogenesis in Drosophila

Genetics ◽  
2001 ◽  
Vol 157 (3) ◽  
pp. 1227-1244 ◽  
Author(s):  
Steffi Kuhfittig ◽  
János Szabad ◽  
Gunnar Schotta ◽  
Jan Hoffmann ◽  
Endre Máthé ◽  
...  
Keyword(s):  
Germ Line ◽  
Position Effect ◽  
Early Embryogenesis ◽  
Position Effect Variegation ◽  
Chromatin Regulation ◽  
Gain Of Function ◽  
Position Effects ◽  
Dominant Female ◽  
Effect Variegation ◽  
Female Germ Line

Abstract The vast majority of the >100 modifier genes of position-effect variegation (PEV) in Drosophila have been identified genetically as haplo-insufficient loci. Here, we describe pitkinDominant (ptnD), a gain-of-function enhancer mutation of PEV. Its exceptionally strong enhancer effect is evident as elevated spreading of heterochromatin-induced gene silencing along euchromatic regions in variegating rearrangements. The ptnD mutation causes ectopic binding of the SU(VAR)3-9 heterochromatin protein at many euchromatic sites and, unlike other modifiers of PEV, it also affects stable position effects. Specifically, it induces silencing of white+ transgenes inserted at a wide variety of euchromatic sites. ptnD is associated with dominant female sterility. +/+ embryos produced by ptnD/+ females mated with wild-type males die at the end of embryogenesis, whereas the ptnD/+ sibling embryos arrest development at cleavage cycle 1-3, due to a combined effect of maternally provided mutant product and an early zygotic lethal effect of ptnD. This is the earliest zygotic effect of a mutation so far reported in Drosophila. Germ-line mosaics show that ptn+ function is required for normal development in the female germ line. These results, together with effects on PEV and white+ transgenes, are consistent with the hypothesis that the ptn gene plays an important role in chromatin regulation during development of the female germ line and in early embryogenesis.

Dedicated protection for the female germ line

2006 ◽  
Vol 8 (1) ◽  
pp. 8-8
Author(s):  
Magdalena Skipper
Keyword(s):  
Germ Line ◽  
Female Germ Line

Multiple products from the shavenbaby-ovo gene region of Drosophila melanogaster: relationship to genetic complexity

1994 ◽  
Vol 14 (10) ◽  
pp. 6809-6818
Author(s):  
M D Garfinkel ◽  
J Wang ◽  
Y Liang ◽  
A P Mahowald
Keyword(s):  
Drosophila Melanogaster ◽  
Germ Line ◽  
Gene Region ◽  
Multiple Products ◽  
Zinc Finger Motifs ◽  
Genetic Complexity ◽  
Complex Locus ◽  
Female Germ Line ◽  
Sensory Structures ◽  
Pole Cells

The Drosophila melanogaster shavenbaby (svb)-ovo gene region is a complex locus, containing two distinct but comutable genetic functions. ovo is required for survival and differentiation of female germ line cells and plays a role in germ line sex determination. In contrast, svb is required in both male and female embryos for the production of epidermal locomotor and sensory structures. Sequences required for the two genetic functions are partially overlapping. ovo corresponds to a previously described germ line-dependent 5.0-kb poly(A)+ mRNA that first appears in the germarium and accumulates in nurse cells during oogenesis. The 5.0-kb mRNA is stored in the egg, but it is rapidly lost in the embryos except for its continued presence in the germ line precursor pole cells. The ovo mRNA predicts a 1,028-amino-acid 110.6-kDa protein homologous with transcription factors. We have identified an embryonic mRNA, 7.1 kb in length, that contains exons partially overlapping those of the 5.0-kb poly(A)+ mRNA. The spatial distribution of this newly discovered transcript during midembryogenesis suggests that it corresponds to the svb function. The arrangement of exons common to the 5.0- and 7.1-kb mRNAs suggests that the Ovo and Svb proteins share DNA-binding specificity conferred by four Cys2-His2 zinc finger motifs but differ functionally in their capacity to interact with other components of the transcription machinery.

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