scholarly journals Xenotransplantation of Human glioblastoma in Zebrafish larvae: in vivo imaging and proliferation assessment

Biology Open ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. bio043257 ◽  
Author(s):  
Luis A. Vargas-Patron ◽  
Nathalie Agudelo-Dueñas ◽  
Jorge Madrid-Wolff ◽  
Juan A. Venegas ◽  
John M. González ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Human Glioblastoma ◽  
Zebrafish Larvae

In vivo imaging of Mauthner axon regeneration, remyelination and synapses re-establishment after laser axotomy in zebrafish larvae

2018 ◽  
Vol 300 ◽  
pp. 67-73 ◽  
Author(s):  
Bing-bing Hu ◽  
Min Chen ◽  
Rong-chen Huang ◽  
Yu-bin Huang ◽  
Yang Xu ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Axon Regeneration ◽  
Zebrafish Larvae

In Vivo Imaging of Mitochondria in Intact Zebrafish Larvae

2014 ◽  
pp. 151-164 ◽  
Author(s):  
Dominik Paquet ◽  
Gabriela Plucińska ◽  
Thomas Misgeld
Keyword(s):  
In Vivo Imaging ◽  
Zebrafish Larvae

Combined in vivo imaging and omics approaches reveal metabolism of icaritin and its glycosides in zebrafish larvae

2011 ◽  
Vol 7 (7) ◽  
pp. 2128 ◽  
Author(s):  
Zhen Hua Li ◽  
Deepa Alex ◽  
Shiu On Siu ◽  
Ivan Keung Chu ◽  
Joerg Renn ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Zebrafish Larvae

MicroPET/CT In Vivo Imaging of Orthotopic Human Glioblastoma Multiforme (hGBM) Murine Model

2010 ◽  
Vol 78 (3) ◽  
pp. S166-S167
Author(s):  
S.S. Park ◽  
J. Chunta ◽  
I. Siiner-Grills ◽  
A.A. Martinez ◽  
G.D. Wilson ◽  
...  
Keyword(s):  
Glioblastoma Multiforme ◽  
Murine Model ◽  
In Vivo Imaging ◽  
Human Glioblastoma ◽  
Human Glioblastoma Multiforme

Fluorescent proteins for in vivo imaging, where's the biliverdin?

2020 ◽  
Vol 48 (6) ◽  
pp. 2657-2667
Author(s):  
Felipe Montecinos-Franjola ◽  
John Y. Lin ◽  
Erik A. Rodriguez
Keyword(s):  
Hydrogen Peroxide ◽  
In Vivo Imaging ◽  
Near Infrared ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescent Imaging ◽  
Infrared Light ◽  
Red Fluorescent Protein ◽  
Color Palette

Noninvasive fluorescent imaging requires far-red and near-infrared fluorescent proteins for deeper imaging. Near-infrared light penetrates biological tissue with blood vessels due to low absorbance, scattering, and reflection of light and has a greater signal-to-noise due to less autofluorescence. Far-red and near-infrared fluorescent proteins absorb light >600 nm to expand the color palette for imaging multiple biosensors and noninvasive in vivo imaging. The ideal fluorescent proteins are bright, photobleach minimally, express well in the desired cells, do not oligomerize, and generate or incorporate exogenous fluorophores efficiently. Coral-derived red fluorescent proteins require oxygen for fluorophore formation and release two hydrogen peroxide molecules. New fluorescent proteins based on phytochrome and phycobiliproteins use biliverdin IXα as fluorophores, do not require oxygen for maturation to image anaerobic organisms and tumor core, and do not generate hydrogen peroxide. The small Ultra-Red Fluorescent Protein (smURFP) was evolved from a cyanobacterial phycobiliprotein to covalently attach biliverdin as an exogenous fluorophore. The small Ultra-Red Fluorescent Protein is biophysically as bright as the enhanced green fluorescent protein, is exceptionally photostable, used for biosensor development, and visible in living mice. Novel applications of smURFP include in vitro protein diagnostics with attomolar (10−18 M) sensitivity, encapsulation in viral particles, and fluorescent protein nanoparticles. However, the availability of biliverdin limits the fluorescence of biliverdin-attaching fluorescent proteins; hence, extra biliverdin is needed to enhance brightness. New methods for improved biliverdin bioavailability are necessary to develop improved bright far-red and near-infrared fluorescent proteins for noninvasive imaging in vivo.

In vivo imaging of beta-amyloid load in transgenic rodents with [F-18]FDDNP microPET

2005 ◽  
Vol 25 (1_suppl) ◽  
pp. S588-S588
Author(s):  
Vladimir Kepe ◽  
Gregory M Cole ◽  
Jie Liu ◽  
Dorothy G Flood ◽  
Stephen P Trusko ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Beta Amyloid ◽  
Amyloid Load

In vivo imaging of liver injury and regeneration by functional two-photon microscopy

2016 ◽  
Vol 54 (12) ◽  
pp. 1343-1404
Author(s):  
A Ghallab ◽  
R Reif ◽  
R Hassan ◽  
AS Seddek ◽  
JG Hengstler
Keyword(s):  
Liver Injury ◽  
In Vivo Imaging ◽  
Two Photon Microscopy ◽  
Two Photon
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