scholarly journals Role of murine macrophage in temporal regulation of cortisol- and serotonin-induced adipogenesis in pre-adipocytes when grown together

Biology Open ◽  
2018 ◽  
Vol 7 (8) ◽  
pp. bio034629 ◽  
Author(s):  
Sushri Priyadarshini ◽  
Biswaranjan Pradhan ◽  
Palok Aich
Keyword(s):  
Murine Macrophage ◽  
Temporal Regulation

scholarly journals BRN2 is a non-canonical melanoma tumor-suppressor

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Michael Hamm ◽  
Pierre Sohier ◽  
Valérie Petit ◽  
Jérémy H. Raymond ◽  
Véronique Delmas ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcription Factors ◽  
Tumor Suppressor ◽  
The Other ◽  
Pi3k Signaling ◽  
Melanoma Tumor ◽  
Temporal Regulation ◽  
Metastatic Colonization ◽  
Pou Domain

AbstractWhile the major drivers of melanoma initiation, including activation of NRAS/BRAF and loss of PTEN or CDKN2A, have been identified, the role of key transcription factors that impose altered transcriptional states in response to deregulated signaling is not well understood. The POU domain transcription factor BRN2 is a key regulator of melanoma invasion, yet its role in melanoma initiation remains unknown. Here, in a BrafV600EPtenF/+ context, we show that BRN2 haplo-insufficiency promotes melanoma initiation and metastasis. However, metastatic colonization is less efficient in the absence of Brn2. Mechanistically, BRN2 directly induces PTEN expression and in consequence represses PI3K signaling. Moreover, MITF, a BRN2 target, represses PTEN transcription. Collectively, our results suggest that on a PTEN heterozygous background somatic deletion of one BRN2 allele and temporal regulation of the other allele elicits melanoma initiation and progression.

scholarly journals Differential spatio-temporal regulation of MMPs in the 5xFAD mouse model of Alzheimer’s disease: evidence for a pro-amyloidogenic role of MT1-MMP

2014 ◽  
Vol 6 ◽  
Author(s):  
Nathalie A. Py ◽  
Amandine E. Bonnet ◽  
Anne Bernard ◽  
Yannick Marchalant ◽  
Eliane Charrat ◽  
...  
Keyword(s):  
Mouse Model ◽  
Temporal Regulation ◽  
5Xfad Mouse ◽  
Spatio Temporal

How the Hox gene Ultrabithorax specifies two different segments: the significance of spatial and temporal regulation within metameres

Development ◽  
1995 ◽  
Vol 121 (9) ◽  
pp. 2973-2982 ◽  
Author(s):  
J. Castelli-Gair ◽  
M. Akam
Keyword(s):  
Limb Development ◽  
Molecular Level ◽  
Early Embryo ◽  
Hox Gene ◽  
Temporal Regulation ◽  
Downstream Target ◽  
Embryo Cells ◽  
Multiple Threshold ◽  
Different Levels

In Drosophila, the Hox gene Ultrabithorax (Ubx) specifies the development of two different metameres--parasegment 5, which is entirely thoracic, and parasegment 6, which includes most of the first abdominal segment. Here we investigate how a single Hox gene can specify two such different morphologies. We show that, in the early embryo, cells respond similarly to UBX protein in both parasegments. The differences between parasegments 5 and 6 can be explained by the different spatial and temporal pattern of UBX protein expression in these two metameres. We find no evidence for multiple threshold responses to different levels of UBX protein. We examine in particular the role of Ubx in limb development. We show that UBX protein will repress limb primordia before 7 hours, when Ubx is expressed in the abdomen, but not later, when UBX is first expressed in the T3 limb primordium. The regulation of one downstream target of UBX, the Distalless gene, provides a model for this transition at the molecular level.

Differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes

1987 ◽  
Vol 87 (1) ◽  
pp. 113-119
Author(s):  
M.J. Smith ◽  
G.L. Koch
Keyword(s):  
Differential Expression ◽  
Surface Glycoprotein ◽  
Murine Macrophage ◽  
Intracellular Pool ◽  
Intracellular Membranes ◽  
Immunofluorescence Studies ◽  
Glycoprotein Antigen ◽  
Tumour Cell Lines ◽  
Punctate Pattern

A monoclonal antibody recognizing a novel murine macrophage glycoprotein antigen (MAG) was prepared by the hybridoma technique. Using live and permeabilized macrophages to estimate surface and total MAG, respectively, it was found that at least 50% of the total antigen was intracellular. In contrast, another macrophage surface glycoprotein antigen, Mac-1, was undetectable in the intracellular pool. Immunofluorescence studies confirmed the existence of a substantial intracellular pool of MAG antigen. Similar results were obtained with a panel of cultured tumour cell lines. In one such cell line, it was shown that surface MAG existed in a distinct punctate pattern indicative of microdomains, whereas surface Mac-1 antigen gave a uniform distribution. The possible role of the surface microdomains in the differential expression of the two surface glycoproteins in intracellular membranes is discussed.

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