scholarly journals The role of integrin β1 in the heterogeneity of human embryonic stem cells culture

Biology Open ◽  
2018 ◽  
Vol 7 (11) ◽  
pp. bio034355 ◽  
Author(s):  
Ade Kallas-Kivi ◽  
Annika Trei ◽  
Artjom Stepanjuk ◽  
Katrin Ruisu ◽  
Keiu Kask ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Integrin Β1

scholarly journals 236. Role of P53 on T Lymphopoiesis from Human Embryonic Stem Cells

2016 ◽  
Vol 24 ◽  
pp. S92-S93
Author(s):  
Hiroshi Kohara ◽  
Jiyuan Liao ◽  
Shohei Miyamoto ◽  
Yoko Nagai ◽  
Tomotoshi Marumoto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
T Lymphopoiesis

A PRC2-Dependent Repressive Role of PRDM14 in Human Embryonic Stem Cells and Induced Pluripotent Stem Cell Reprogramming

Stem Cells ◽  
2013 ◽  
Vol 31 (4) ◽  
pp. 682-692 ◽  
Author(s):  
Yun-Shen Chan ◽  
Jonathan Göke ◽  
Xinyi Lu ◽  
Nandini Venkatesan ◽  
Bo Feng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Pluripotent Stem Cell ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Cell Reprogramming ◽  
Induced Pluripotent

scholarly journals Role of MicroRNAs 99b, 181a, and 181b in the Differentiation of Human Embryonic Stem Cells to Vascular Endothelial Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (4) ◽  
pp. 643-654 ◽  
Author(s):  
Nicole M. Kane ◽  
Lynsey Howard ◽  
Betty Descamps ◽  
Marco Meloni ◽  
John McClure ◽  
...  
Keyword(s):  
Stem Cells ◽  
Endothelial Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Vascular Endothelial Cells ◽  
Embryonic Stem ◽  
Vascular Endothelial

scholarly journals Regulated expression of microRNAs-126/126* inhibits erythropoiesis from human embryonic stem cells

Blood ◽  
2011 ◽  
Vol 117 (7) ◽  
pp. 2157-2165 ◽  
Author(s):  
Xinqiang Huang ◽  
Eric Gschweng ◽  
Ben Van Handel ◽  
Donghui Cheng ◽  
Hanna K. A. Mikkola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Protein Tyrosine Phosphatase ◽  
Embryoid Body ◽  
Tyrosine Phosphatase ◽  
Embryonic Stem ◽  
Hematopoietic Differentiation ◽  
Protein Tyrosine

Abstract MicroRNAs (miRs) play an important role in cell differentiation and maintenance of cell identity, but relatively little is known of their functional role in modulating human hematopoietic lineage differentiation. Human embryonic stem cells (hESCs) provide a model system to study early human hematopoiesis. We differentiated hESCs by embryoid body (EB) formation and compared the miR expression profile of undifferentiated hESCs to CD34+ EB cells. miRs-126/126* were the most enriched of the 7 miRs that were up-regulated in CD34+ cells, and their expression paralleled the kinetics of hematopoietic transcription factors RUNX1, SCL, and PU.1. To define the role of miRs-126/126* in hematopoiesis, we created hESCs overexpressing doxycycline-regulated miRs-126/126* and analyzed their hematopoietic differentiation. Induction of miRs-126/126* during both EB differentiation and colony formation reduced the number of erythroid colonies, suggesting an inhibitory role of miRs-126/126* in erythropoiesis. Protein tyrosine phosphatase, nonreceptor type 9 (PTPN9), a protein tyrosine phosphatase that is required for growth and expansion of erythroid cells, is one target of miR-126. PTPN9 restoration partially relieved the suppressed erythropoiesis caused by miRs-126/126*. Our results define an important function of miRs-126/126* in negative regulation of erythropoiesis, providing the first evidence for a role of miR in hematopoietic differentiation of hESCs.

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