scholarly journals Survival of glucose phosphate isomerase null somatic cells and germ cells in adult mouse chimaeras

Biology Open ◽  
2016 ◽  
Vol 5 (5) ◽  
pp. 596-610 ◽  
Author(s):  
Margaret A. Keighren ◽  
Jean H. Flockhart ◽  
John D. West
Keyword(s):  
Germ Cells ◽  
Adult Mouse ◽  
Somatic Cells ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate

Germ cell nuclei of male fetal mice can support development of chimeras to midgestation following serial transplantation

Development ◽  
1995 ◽  
Vol 121 (3) ◽  
pp. 779-783 ◽  
Author(s):  
Y. Kato ◽  
Y. Tsunoda
Keyword(s):  
Germ Cells ◽  
Nuclear Transfer ◽  
Yolk Sac ◽  
Glucose Phosphate Isomerase ◽  
Cell Nuclei ◽  
Cell Stage ◽  
Glucose Phosphate ◽  
Fetal Mice ◽  
Serial Nuclear Transfer

Chimeric embryos between fertilized eggs from F1 (C57BL × CBA) and 15.5-16.5 days post coitum (dpc) male fetal germ cells (FGCs) from CD-1 strain (glucose phosphate isomerase, Gpi-1a/a) mice were produced by nuclear transfer. Briefly, a single FGC was fused with enucleated oocytes and activated, and the reconstituted oocytes were cultured to the 2-cell stage. The nucleus from the reconstituted 2-cell embryos was then transferred into an enucleated blastomere of the same stage embryos derived from F1 mice to produce chimeric embryos. The reconstituted 2-cell embryos, which synchronously divided to the 4-cell stage after treatment with nocodazole, were further cultured in vitro. Compacted morula and blastocysts were transferred to the uteri of pseudopregnant female mice. Some recipients were allowed to develop to term and the others were killed at mid gestation to analyze the contribution of donor FGC-derived cells. Survival to term was low with no chimeric animals. Glucose phosphate isomerase (GPI) analysis at midgestation revealed that some conceptuses had chimerism in the fetuses, trophoblast and yolk sac at day 10.5 of pregnancy. The contribution of donor cells was 37–47%, 19–65% and 12–63%, respectively. It was concluded that the nucleus from 15.5-16.5 dpc male fetal germ cells had the potency to develop into fetus, trophoblast and yolk sac after serial nuclear transfer with oocytes and fertilized embryos. The reason for the low viability of chimeric embryos is discussed.

scholarly journals GC-1 spg cells are most similar to Leydig cells, a testis somatic cell-type, and not germ cells

2021 ◽  
Author(s):  
Andrew R Norman ◽  
Lauren Byrnes ◽  
Jeremy R Reiter
Keyword(s):  
Germ Cell ◽  
Somatic Cell ◽  
Cell Line ◽  
Germ Cells ◽  
Leydig Cells ◽  
Adult Mouse ◽  
Somatic Cells ◽  
Cell Type ◽  
Immortalized Cell ◽  
Somatic Cell Type

GC-1 spg is an immortalized cell line derived from an adult mouse testis and reported to be most similar to spermatocytes, a male germ cell-type. However, immunofluorescence indicates that GC-1 spg cells express WT1, a marker of testis somatic cells, and do not express markers of germ cells. Transcriptomic profiling indicate GC-1 cells are most similar to Leydig cells. Therefore, we conclude that GC-1 spg cells are most similar to testis somatic cells.

scholarly journals GPI expression in female germ cells of the mouse

1981 ◽  
Vol 37 (3) ◽  
pp. 303-309 ◽  
Author(s):  
Anne McLaren ◽  
Mia Buehr
Keyword(s):  
Structural Gene ◽  
Germ Cells ◽  
Gene Complex ◽  
Glucose Phosphate Isomerase ◽  
Specific Expression ◽  
Isomerase Activity ◽  
Glucose Phosphate ◽  
Form Part ◽  
Genetically Determined

SUMMARYThe genetically determined oocyte-specific expression of glucose-phosphate isomerase activity in the mouse is first apparent at 6 to 7 days after birth, and occurs in XO as well as in XX oocytes. The regulator locus that controls oocyte-specific expression shows the same linkage relations as the structural gene, suggesting that both form part of a Gpi-1 gene complex.

Characterization of the 5′ end of the gene for human glucose phosphate isomerase (GPI)

Genomics ◽  
1990 ◽  
Vol 7 (4) ◽  
pp. 638-643 ◽  
Author(s):  
James I.H. Walker ◽  
Pelin Faik ◽  
Michael J. Morgan
Keyword(s):  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate

scholarly journals Müllerian Inhibiting Substance Is Required for Germ Cell Proliferation during Early Gonadal Differentiation in Medaka (Oryzias latipes)

Endocrinology ◽  
2007 ◽  
Vol 149 (4) ◽  
pp. 1813-1819 ◽  
Author(s):  
Eri Shiraishi ◽  
Norifumi Yoshinaga ◽  
Takeshi Miura ◽  
Hayato Yokoi ◽  
Yuko Wakamatsu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Sex Differentiation ◽  
Somatic Cells ◽  
Oryzias Latipes ◽  
Cell Number ◽  
Gonadal Differentiation ◽  
Loss Of Function ◽  
Germ Cell Proliferation

Müllerian inhibiting substance (MIS) is a glycoprotein belonging to the TGF-β superfamily. In mammals, MIS is responsible for the regression of Müllerian ducts in the male fetus. However, the role of MIS in gonadal sex differentiation of teleost fish, which have no Müllerian ducts, has yet to be clarified. In the present study, we examined the expression pattern of mis and mis type 2 receptor (misr2) mRNAs and the function of MIS signaling in early gonadal differentiation in medaka (teleost, Oryzias latipes). In situ hybridization showed that both mis and misr2 mRNAs were expressed in the somatic cells surrounding the germ cells of both sexes during early sex differentiation. Loss-of-function of either MIS or MIS type II receptor (MISRII) in medaka resulted in suppression of germ cell proliferation during sex differentiation. These results were supported by cell proliferation assay using 5-bromo-2′-deoxyuridine labeling analysis. Treatment of tissue fragments containing germ cells with recombinant eel MIS significantly induced germ cell proliferation in both sexes compared with the untreated control. On the other hand, culture of tissue fragments from the MIS- or MISRII-defective embryos inhibited proliferation of germ cells in both sexes. Moreover, treatment with recombinant eel MIS in the MIS-defective embryos dose-dependently increased germ cell number in both sexes, whereas in the MISRII-defective embryos, it did not permit proliferation of germ cells. These results suggest that in medaka, MIS indirectly stimulates germ cell proliferation through MISRII, expressed in the somatic cells immediately after they reach the gonadal primordium.

scholarly journals Fertility of mice receiving vitrified adult mouse ovaries

Reproduction ◽  
2006 ◽  
Vol 131 (4) ◽  
pp. 681-687 ◽  
Author(s):  
Toshio Hani ◽  
Takanori Tachibe ◽  
Saburo Shingai ◽  
Nobuo Kamada ◽  
Otoya Ueda ◽  
...  
Keyword(s):  
Germ Cells ◽  
Adult Mouse ◽  
Green Fluorescence Protein ◽  
Green Fluorescence ◽  
Experimental Animals ◽  
Immature Mouse ◽  
Estrous Cyclicity ◽  
Fluorescence Protein ◽  
High Degree ◽  
Old Mice

Cryopreservation of the ovaries is a useful technology for preservation of germ cells from experimental animals, because if the female founder is infertile or has mutated mitochondrial DNA, preservation of female germ cells is necessary. Although it is possible to cryopreserve immature mouse ovaries with a high degree of viability by vitrification with a mixture of several cryoprotectants, the viability of cryopreserved adult mouse ovaries is still unknown. Here, we investigated the viability of mouse ovaries at various ages after cryopreservation by vitrification techniques. Donor ovaries were collected from 10-day-, 4-week-, 10-week- and 7-month-old, female, nulliparous, green fluorescence protein (GFP)-transgenic mice and cryopreserved by vitrification. The vitrified-warmed ovaries were orthotopically transplanted to 4- or 10-week-old mice. GFP-positive pups were obtained in all experimental groups. In the 4-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 44%, 9%, 12% and 4% respectively. In the 10-week-old recipients, the percentages of GFP-positive pups among the total pups from recipients transplanted with ovaries of 10-day-, 4-week-, 10-week- and 7-month-old donors were 36%, 16%, 2% and 9% respectively. Furthermore, GFP-positive pups also were obtained from recipients transplanted with ovaries of donors without normal estrous cyclicity. Our results indicate that cryopreservation of mouse ovaries by vitrification is a useful method for the preservation of female germ cells from mice of various ages.

Neonatal administration of diethylstilbestrol has adverse effects on somatic cells rather than germ cells

2006 ◽  
Vol 22 (4) ◽  
pp. 746-753 ◽  
Author(s):  
Kyu-Bom Koh ◽  
Yoshiro Toyama ◽  
Masatoshi Komiyama ◽  
Tetsuya Adachi ◽  
Hideki Fukata ◽  
...  
Keyword(s):  
Adverse Effects ◽  
Germ Cells ◽  
Somatic Cells ◽  
Neonatal Administration

Comparison of human and murine isolates of Schistosoma mansoni from Richard-Toll, Senegal, by isoelectric focusing

1997 ◽  
Vol 71 (2) ◽  
pp. 175-181 ◽  
Author(s):  
M. Sène ◽  
P. Brémond ◽  
J.P. Hervé ◽  
V.R. Southgate ◽  
B. Sellin ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Acid Phosphatase ◽  
Lactate Dehydrogenase ◽  
Schistosoma Mansoni ◽  
Isoelectric Focusing ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate ◽  
Enzyme Systems ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Arvicanthis Niloticus

AbstractStudies on human and murine isolates of Schistosoma mansoni, from Richard-Toll, Senegal, were carried out by isoelectric focusing in polyacrylamide gels. Seven enzyme systems; lactate dehydrogenase (LDH), malate dehydrogenase (MDH), glucose-6-phosphate dehydrogenase (G6PD), acid phosphatase (AcP), hexokinase (HK), glucose phosphate isomerase (GPI), and phosphoglucomutase (PGM), were used to compare the two isolates. All systems tested, apart from LDH, were found to be polymorphic for both isolates. Interestingly, one phenotype is more frequent than the remainder. The results show that there is no significant genetic variation between the S. mansoni isolates from man and the rodents, Arvicanthis niloticus and Mastomys huberti.

Glucose Phosphate Isomerase Deficiency as a Cause of Hydrops Fetalis

1987 ◽  
Vol 316 (5) ◽  
pp. 258-261 ◽  
Author(s):  
Yaddanapudi Ravindranath ◽  
Donald E. Paglia ◽  
Indira Warrier ◽  
William Valentine ◽  
Misae Nakatani ◽  
...  
Keyword(s):  
Hydrops Fetalis ◽  
Glucose Phosphate Isomerase ◽  
Glucose Phosphate
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