scholarly journals Tissue-specific tagging of endogenous loci inDrosophila melanogaster

Biology Open ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Kate Koles ◽  
Anna R. Yeh ◽  
Avital A. Rodal
Keyword(s):  
Tissue Specific ◽  
Endogenous Loci

scholarly journals Tissue-specific tagging of endogenous loci inDrosophila melanogaster

2015 ◽  
Author(s):  
Kate Koles ◽  
Anna R Yeh ◽  
Avital A Rodal
Keyword(s):  
Protein Function ◽  
Fluorescent Protein ◽  
Endocytic Pathway ◽  
Expression Systems ◽  
Tissue Specific ◽  
Efficient Gene ◽  
Endogenous Protein ◽  
Endogenous Proteins ◽  
Endogenous Loci ◽  
Fluorescent Protein Tags

Fluorescent protein tags have revolutionized cell and developmental biology, and in combination with binary expression systems they enable diverse tissue-specific studies of protein function. However these binary expression systems often do not recapitulate endogenous protein expression levels, localization, binding partners, and developmental windows of gene expression. To address these limitations, we have developed a method called T-STEP (Tissue-SpecificTagging ofEndogenousProteins) that allows endogenous loci to be tagged in a tissue specific manner. T-STEP uses a combination of efficient gene targeting and tissue-specific recombinase-mediated tag swapping to temporally and spatially label endogenous proteins. We have employed this method to GFP tag OCRL (a phosphoinositide-5-phosphatase in the endocytic pathway) and Vps35 (a Parkinson’s disease-implicated component of the endosomal retromer complex) in diverse Drosophila tissues including neurons, glia, muscles, and hemocytes. Selective tagging of endogenous proteins allows for the first time cell type-specific live imaging and proteomics in complex tissues.

Expression of sunflower cytochrome c mRNA is tissue-specific and controlled by nitrate and light

1997 ◽  
Vol 99 (2) ◽  
pp. 342-347 ◽  
Author(s):  
Silvina A. Felitti ◽  
Raquel L. Chan ◽  
Gabriela Gago ◽  
Estela M. Valle ◽  
Daniel H. Gonzalez
Keyword(s):  
Cytochrome C ◽  
Tissue Specific

Tissue-specific expression patterns of nuclear receptors

2013 ◽  
Author(s):  
AL Bookout ◽  
Y Jeong ◽  
M Downes ◽  
RT Yu ◽  
RM Evans ◽  
...  
Keyword(s):  
Nuclear Receptors ◽  
Expression Patterns ◽  
Specific Expression ◽  
Tissue Specific ◽  
Tissue Specific Expression
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