Upstream stimulatory factor, a basic-helix-loop-helix-zipper protein, regulates the activity of the alpha-glycoprotein hormone subunit gene in pituitary cells

1995 ◽  
Vol 9 (3) ◽  
pp. 278-291 ◽  
Author(s):  
S. M. Jackson
Keyword(s):  
Pituitary Cells ◽  
Subunit Gene ◽  
Upstream Stimulatory Factor ◽  
Glycoprotein Hormone ◽  
Basic Helix Loop Helix ◽  
Helix Loop Helix ◽  
Stimulatory Factor

scholarly journals Cell-Specific Expression of the Mouse Glycoprotein Hormone α-Subunit Gene Requires Multiple Interacting DNA Elements in Transgenic Mice and Cultured Cells

1998 ◽  
Vol 12 (5) ◽  
pp. 622-633 ◽  
Author(s):  
Michelle L. Brinkmeier ◽  
David F. Gordon ◽  
Janet M. Dowding ◽  
Thomas L. Saunders ◽  
Susan K. Kendall ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Pituitary Cells ◽  
Subunit Gene ◽  
High Level Expression ◽  
Glycoprotein Hormone ◽  
Α Subunit ◽  
Peripheral Tissues ◽  
Cell Specificity ◽  
Dna Elements ◽  
High Level

Abstract The glycoprotein hormone α-subunit gene is expressed and differentially regulated in pituitary gonadotropes and thyrotropes. Previous gene expression studies suggested that cell specificity may be regulated by distinct DNA elements. We have identified an enhancer region between −4.6 and −3.7 kb that is critical for high level expression in both gonadotrope and thyrotrope cells of transgenic mice. Fusion of the enhancer to −341/+43 mouseα -subunit promoter results in appropriate pituitary cell specificity and transgene expression levels that are similar to levels observed with the intact −4.6 kb/+43 construct. Deletion of sequences between− 341 and −297 resulted in a loss of high level expression and cell specificity, exhibited by ectopic transgene activation in GH-, ACTH-, and PRL-producing pituitary cells as well as in other peripheral tissues. Consistent with these results, transient cell transfection studies demonstrated that the enhancer stimulated activity of a− 341/+43 α-promoter in both αTSH and αT3 cells, but it did not enhance α-promoter activity significantly in CV-1 cells. Removal of sequences between −341 and −297 allowed the enhancer to function in heterologous cells. Loss of high level expression and cell specificity may be due to loss of sequences required for binding of the LIM homeoproteins or the α-basal element 1. These data demonstrate that the enhancer requires participation by both proximal and distal sequences for high level expression and suggests that sequences from− 341 to −297 are critical for restricting expression to the anterior pituitary.

scholarly journals The Basic Helix-Loop-Helix, Leucine Zipper Transcription Factor, USF (Upstream Stimulatory Factor), Is a Key Regulator of SF-1 (Steroidogenic Factor-1) Gene Expression in Pituitary Gonadotrope and Steroidogenic Cells

1998 ◽  
Vol 12 (5) ◽  
pp. 714-726 ◽  
Author(s):  
Adrienne N. Harris ◽  
Pamela L. Mellon
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Transcriptional Regulator ◽  
Orphan Nuclear Receptor ◽  
Upstream Stimulatory Factor ◽  
Specific Expression ◽  
Steroidogenic Factor 1 ◽  
Helix Loop Helix ◽  
E Box ◽  
Stimulatory Factor

Abstract Tissue-specific expression of the mammalian FTZ-F1 gene is essential for adrenal and gonadal development and sexual differentiation. The FTZ-F1 gene encodes an orphan nuclear receptor, termed SF-1 (steroidogenic factor-1) or Ad4BP, which is a primary transcriptional regulator of several hormone and steroidogenic enzyme genes that are critical for normal physiological function of the hypothalamic-pituitary-gonadal axis in reproduction. The objective of the current study is to understand the molecular mechanisms underlying transcriptional regulation of SF-1 gene expression in the pituitary. We have studied a series of deletion and point mutations in the SF-1 promoter region for transcriptional activity in αT3–1 and LβT2 (pituitary gonadotrope), CV-1, JEG-3, and Y1 (adrenocortical) cell lines. Our results indicate that maximal expression of the SF-1 promoter in all cell types requires an E box element at −82/−77. This E box sequence (CACGTG) is identical to the binding element for USF (upstream stimulatory factor), a member of the helix-loop-helix family of transcription factors. Studies of the SF-1 gene E box element using gel mobility shift and antibody supershift assays indicate that USF may be a key transcriptional regulator of SF-1 gene expression.

scholarly journals An upstream stimulatory factor (USF) binding motif is critical for rat preprotachykinin-A promoter activity in PC12 cells

1995 ◽  
Vol 310 (2) ◽  
pp. 401-406 ◽  
Author(s):  
J M Paterson ◽  
C F Morrison ◽  
S C Mendelson ◽  
J McAllister ◽  
J P Quinn
Keyword(s):  
Promoter Activity ◽  
Cell Model ◽  
Nuclear Extract ◽  
Regulatory Proteins ◽  
Binding Motif ◽  
Upstream Stimulatory Factor ◽  
Promoter Fragment ◽  
Helix Loop Helix ◽  
E Box ◽  
Stimulatory Factor

We demonstrate the presence of a functional E box motif in the proximal rat preprotachykinin-A (rPPT) promoter. This element (spanning nucleotides -67 to -47) exhibits the sequence 5′-CACGTG-3′ which is recognized and bound by the basic helix-loop-helix family of regulatory proteins. We also show that at least one of the factors bound to this rPPT promoter element in both HeLa and PC12 nuclear extract is the ubiquitously expressed transcription factor, the upstream stimulatory factor (USF). Mutation of this element by insertion of a 10 bp linker into the E box motif, in an rPPT promoter fragment spanning -865 to +92, destroys the ability of this promoter fragment to support reporter gene expression in a PC12 cell model of rPPT promoter activity. The data indicate that this rPPT E box element is likely to function as an important cis-regulatory domain in the rPPT promoter.

scholarly journals The upstream stimulatory factor binds to and activates the promoter of the rat class I alcohol dehydrogenase gene

1991 ◽  
Vol 266 (23) ◽  
pp. 15457-15463 ◽  
Author(s):  
J.J. Potter ◽  
D. Cheneval ◽  
C.V. Dang ◽  
L.M. Resar ◽  
E. Mezey ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Class I ◽  
Upstream Stimulatory Factor ◽  
Dehydrogenase Gene ◽  
Stimulatory Factor
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