scholarly journals Systematic Determination of Differential Gene Expression in the Primate Corpus Luteum during the Luteal Phase of the Menstrual Cycle

2008 ◽  
Vol 22 (5) ◽  
pp. 1260-1273 ◽  
Author(s):  
Randy L. Bogan ◽  
Melinda J. Murphy ◽  
Richard L. Stouffer ◽  
Jon D. Hennebold
Keyword(s):  
Gene Expression ◽  
Menstrual Cycle ◽  
Corpus Luteum ◽  
Differential Gene Expression ◽  
Luteal Phase ◽  
Systematic Determination ◽  
Differential Gene

scholarly journals 213 Transcriptome profiling of olfactory epithelium in normal cycling and acyclic gilts

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 125-125
Author(s):  
Dan J Nonneman ◽  
Aaron M Dickey ◽  
Clay A Lents
Keyword(s):  
Gene Expression ◽  
Differential Gene Expression ◽  
Olfactory Epithelium ◽  
Luteal Phase ◽  
Follicular Phase ◽  
Hormonal Control ◽  
Differentially Expressed ◽  
Receptor Protein ◽  
Genome Wide Association Studies ◽  
Differential Gene

Abstract A significant proportion of gilts that enter the herd never farrow a litter and are culled because of anestrus or failure to conceive. Genome-wide association studies for pubertal traits have identified loci involved in neuronal and olfactory pathways, and olfaction is critical for expression of reproductive behavior in mammalian females. We evaluated major olfactory epithelium (MOE) for differential gene expression in nonpubertal, behavioral anestrus and normal cycling early follicular and luteal phase gilts (n = 8/group; average age of 259 days). An average of 50 million paired-end RNA-seq reads were collected from each of the 32 RNA libraries and mapped to Sscrofa 11.1. Differential gene expression was determined using DESeq2. A total of 18,484 genes were expressed with a mean normalized expression value greater than 5. Only four genes were differentially expressed between nonpubertal or behavioral anestrus MOE and their cycling controls (early follicular and luteal, respectively). Comparing cycling follicular and luteal phase gilts showed that 1146 genes were more highly expressed in MOE from follicular phase gilts, whereas 1351 genes were more highly expressed in MOE from luteal phase gilts. Pathways for transmembrane receptor protein tyrosine kinase/growth factor signaling, cell junction and epithelium development were overrepresented in follicular phase MOE and cell cycle, chromatin remodeling, inflammatory response and sensory perception were overrepresented in luteal phase MOE. While 1348 locus IDs were identified for olfactory receptors in MOE, only 160 were expressed at an appreciable level (base mean > 5) in MOE and 16 were more highly expressed in MOE from luteal phase than follicular phase gilts. While few genes were differentially expressed in MOE between prepubertal and anestrus gilts and cycling gilts at the same ovarian stage, the comparison between ovarian stages indicates that MOE gene expression is under hormonal control. USDA is an equal opportunity provider and employer.

Differential Gene Expression in Oviducts Collected During Proestrus from Ewes Fasted During the Luteal Phase of the Estrous Cycle.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 109-109
Author(s):  
Kathleen J. Austin ◽  
Kristi M. Cammack ◽  
Edward A. Van Kirk ◽  
Gary E. Moss ◽  
Brenda M. Alexander
Keyword(s):  
Gene Expression ◽  
Estrous Cycle ◽  
Differential Gene Expression ◽  
Luteal Phase ◽  
Differential Gene
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