scholarly journals Contrasting Effects of Two Alternative Splicing Forms of Coactivator-Associated Arginine Methyltransferase 1 on Thyroid Hormone Receptor-Mediated Transcription in Xenopus laevis

2007 ◽  
Vol 21 (5) ◽  
pp. 1082-1094 ◽  
Author(s):  
Hiroki Matsuda ◽  
Bindu D. Paul ◽  
Cheol Young Choi ◽  
Yun-Bo Shi
Keyword(s):  
Alternative Splicing ◽  
Thyroid Hormone ◽  
Xenopus Laevis ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Arginine Methyltransferase ◽  
Methyltransferase 1

scholarly journals Novel Functions of Protein Arginine Methyltransferase 1 in Thyroid Hormone Receptor-Mediated Transcription and in the Regulation of Metamorphic Rate in Xenopus laevis

2008 ◽  
Vol 29 (3) ◽  
pp. 745-757 ◽  
Author(s):  
Hiroki Matsuda ◽  
Bindu D. Paul ◽  
Cheol Young Choi ◽  
Takashi Hasebe ◽  
Yun-Bo Shi
Keyword(s):  
Thyroid Hormone ◽  
Xenopus Laevis ◽  
Thyroid Hormone Receptor ◽  
Protein Arginine Methyltransferase ◽  
Arginine Methyltransferase ◽  
Frog Oocyte ◽  
Protein Arginine Methyltransferase 1 ◽  
Intestinal Remodeling ◽  
Methyltransferase 1

ABSTRACT Protein arginine methyltransferase 1 (PRMT1) acts as a transcription coactivator for nuclear receptors through histone H4 R3 methylation. The in vivo function of PRMT1 is largely unknown. Here we investigated the role of PRMT1 in thyroid hormone (T3) receptor (TR)-mediated transcription in vivo during vertebrate development. By using intestinal remodeling during T3-dependent Xenopus laevis metamorphosis for in vivo molecular analysis, we first showed that PRMT1 expression was upregulated during metamorphosis when both TR and T3 were present. We then demonstrated a role for PRMT1 in TR-mediated transcription by showing that PRMT1 enhanced transcriptional activation by liganded TR in the frog oocyte transcription system and was recruited to the T3 response element (TRE) of the target promoter in the oocyte, as well as to endogenous TREs during frog metamorphosis. Surprisingly, we found that PRMT1 was only transiently recruited to the TREs in the target during metamorphosis and observed no PRMT1 recruitment to TREs at the climax of intestinal remodeling when both PRMT1 and T3 were at peak levels. Mechanistically, we showed that overexpression of PRMT1 enhanced TR binding to TREs both in the frog oocyte model system and during metamorphosis. More importantly, transgenic overexpression of PRMT1 enhanced gene activation in vivo and accelerated both natural and T3-induced metamorphosis. These results thus indicate that PRMT1 functions transiently as a coactivator in TR-mediated transcription by enhancing TR-TRE binding and further suggest that PRMT1 has tissue-specific roles in regulating the rate of metamorphosis.

scholarly journals The thyroid hormone receptor gene (c-erbA alpha) is expressed in advance of thyroid gland maturation during the early embryonic development of Xenopus laevis.

1991 ◽  
Vol 11 (10) ◽  
pp. 5079-5089 ◽  
Author(s):  
D E Banker ◽  
J Bigler ◽  
R N Eisenman
Keyword(s):  
Gene Expression ◽  
Thyroid Hormone ◽  
Thyroid Gland ◽  
Xenopus Laevis ◽  
Hormone Receptor ◽  
Hormone Receptors ◽  
Thyroid Hormone Receptor ◽  
Thyroid Hormone Receptors ◽  
Stages Of Development ◽  
Xenopus Development

The c-erbA proto-oncogene encodes the thyroid hormone receptor, a ligand-dependent transcription factor which plays an important role in vertebrate growth and development. To define the role of the thyroid hormone receptor in developmental processes, we have begun studying c-erbA gene expression during the ontogeny of Xenopus laevis, an organism in which thyroid hormone has well-documented effects on morphogenesis. Using polymerase chain reactions (PCR) as a sensitive assay of specific gene expression, we found that polyadenylated erbA alpha RNA is present in Xenopus cells at early developmental stages, including the fertilized egg, blastula, gastrula, and neurula. By performing erbA alpha-specific PCR on reverse-transcribed RNAs from high-density sucrose gradient fractions prepared from early-stage embryos, we have demonstrated that these erbA transcripts are recruited to polysomes. Therefore, erbA is expressed in Xenopus development prior to the appearance of the thyroid gland anlage in tailbud-stage embryos. This implies that erbA alpha/thyroid hormone receptors may play ligand-independent roles during the early development of X. laevis. Quantitative PCR revealed a greater than 25-fold range in the steady-state levels of polyadenylated erbA alpha RNA across early stages of development, as expressed relative to equimolar amounts of total embryonic RNA. Substantial increases in the levels of erbA alpha RNA were noted at stages well after the onset of zygotic transcription at the mid-blastula transition, with accumulation of erbA alpha transcripts reaching a relative maximum in advance of metamorphosis. We also show that erbA alpha RNAs are expressed unequally across Xenopus neural tube embryos. This differential expression continues through later stages of development, including metamorphosis. This finding suggests that erbA alpha/thyroid hormone receptors may play roles in tissue-specific processes across all of Xenopus development.

scholarly journals Unliganded thyroid hormone receptor is essential for Xenopus laevis eye development

2006 ◽  
Vol 25 (20) ◽  
pp. 4943-4951 ◽  
Author(s):  
Emmanuelle Havis ◽  
Sébastien Le Mevel ◽  
Ghislaine Morvan Dubois ◽  
De-Li Shi ◽  
Thomas S Scanlan ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Xenopus Laevis ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Eye Development

Expression of thyroid hormone receptor βA gene assayed by transgenic Xenopus laevis carrying its promoter sequences

2001 ◽  
Vol 181 (1-2) ◽  
pp. 97-110 ◽  
Author(s):  
Ken Oofusa ◽  
Osamu Tooi ◽  
Akihiko Kashiwagi ◽  
Keiko Kashiwagi ◽  
Yasuyuki Kondo ◽  
...  
Keyword(s):  
Thyroid Hormone ◽  
Xenopus Laevis ◽  
Hormone Receptor ◽  
Thyroid Hormone Receptor ◽  
Promoter Sequences
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