scholarly journals Adaptor Protein SH2-B Linking Receptor-Tyrosine Kinase and Akt Promotes Adipocyte Differentiation by Regulating Peroxisome Proliferator-Activated Receptor γ Messenger Ribonucleic Acid Levels

2007 ◽  
Vol 21 (5) ◽  
pp. 1120-1131 ◽  
Author(s):  
Daigo Yoshiga ◽  
Naoichi Sato ◽  
Takehiro Torisu ◽  
Hiroyuki Mori ◽  
Ryoko Yoshida ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
Receptor Tyrosine Kinase ◽  
Ribonucleic Acid ◽  
Adipocyte Differentiation ◽  
Adaptor Protein ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Messenger Ribonucleic Acid

scholarly journals Is the Mitochondrial Function of Keloid Fibroblasts Affected by Cytoglobin?

2021 ◽  
Vol 28 (2) ◽  
pp. 39-47
Author(s):  
Sri Widia A Jusman ◽  
Isma Nur Azzizah ◽  
Mohamad Sadikin ◽  
Novi Silvia Hardiany
Keyword(s):  
Mitochondrial Biogenesis ◽  
Mitochondrial Function ◽  
Ribonucleic Acid ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Messenger Ribonucleic Acid ◽  
Keloid Fibroblast ◽  
Excessive Proliferation ◽  
Keloid Fibroblasts

Background: A keloid is a benign skin tumour characterised by excessive proliferation of fibroblasts, a process that requires a sufficient amount of energy. The energy needs are associated with adequate oxygen (O2) flow and well-functioning mitochondria. It is known that cytoglobin (CYGB) has a function in O2 distribution. The aim of the present study was to explore whether the inhibition of CYGB expression caused impaired mitochondrial function of keloid fibroblasts. Methods: An in vitro study was conducted on a keloid fibroblast derived from our previous study. The study was carried out in the laboratory of the Biochemistry & Molecular Biology Department, Faculty of Medicine, Universitas Indonesia (FMUI), from July to December 2018. CYGB expression was inhibited by small interfering ribonucleic acid (siRNA) and CYGB. Analysis of mitochondrial function was observed through peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α), a mitochondrial biogenesis marker and the activity of the succinate dehydrogenase (SDH) enzyme in mitochondria. Results: The CYGB gene and protein were downregulated after treatment with CYGB siRNA. Inhibition of CYGB expression with siRNA also tended to decrease the levels of PGC-1α messenger ribonucleic acid (mRNA) and protein, as well as SDH enzyme activity. Conclusion: Inhibition of CYGB expression with siRNA tended to decrease mitochondrial biogenesis and function. This may be useful for understanding the excessive proliferation of fibroblasts in keloids and for development of treatment for keloids.

scholarly journals Expression of Peroxisome Proliferator-Activated Receptor α Messenger Ribonucleic Acid and Protein in Human and Rat Testis

Endocrinology ◽  
1999 ◽  
Vol 140 (7) ◽  
pp. 2968-2975 ◽  
Author(s):  
Rüdiger Schultz ◽  
Wei Yan ◽  
Jorma Toppari ◽  
Alfred Völkl ◽  
Jan-Åke Gustafsson ◽  
...  
Keyword(s):  
Ribonucleic Acid ◽  
Peroxisome Proliferator ◽  
Peroxisome Proliferator Activated Receptor ◽  
Messenger Ribonucleic Acid ◽  
Rat Testis
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