scholarly journals Protein Kinase C-λ Knockout in Embryonic Stem Cells and Adipocytes Impairs Insulin-Stimulated Glucose Transport

2004 ◽  
Vol 18 (2) ◽  
pp. 373-383 ◽  
Author(s):  
Gautam Bandyopadhyay ◽  
Mary L. Standaert ◽  
Mini P. Sajan ◽  
Yoshinori Kanoh ◽  
Atsushi Miura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Glucose Transport ◽  
Embryonic Stem ◽  
Kinase C

scholarly journals Self-Renewal Versus Lineage Commitment of Embryonic Stem Cells: Protein Kinase C Signaling Shifts the Balance

Stem Cells ◽  
2011 ◽  
Vol 29 (4) ◽  
pp. 618-628 ◽  
Author(s):  
Debasree Dutta ◽  
Soma Ray ◽  
Pratik Home ◽  
Melissa Larson ◽  
Michael W. Wolfe ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Embryonic Stem ◽  
Lineage Commitment ◽  
Self Renewal ◽  
Kinase C

scholarly journals Protein Kinase C Signaling Transduces Endorphin-Primed Cardiogenesis in GTR1 Embryonic Stem Cells

2003 ◽  
Vol 92 (6) ◽  
pp. 617-622 ◽  
Author(s):  
Carlo Ventura ◽  
Elisabetta Zinellu ◽  
Emiliana Maninchedda ◽  
Marina Fadda ◽  
Margherita Maioli
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Embryonic Stem ◽  
Kinase C

scholarly journals Protein Kinase C Mediated Extraembryonic Endoderm Differentiation of Human Embryonic Stem Cells

Stem Cells ◽  
2012 ◽  
Vol 30 (3) ◽  
pp. 461-470 ◽  
Author(s):  
Xuezhu Feng ◽  
Jiuchun Zhang ◽  
Kimberly Smuga-Otto ◽  
Shulan Tian ◽  
Junying Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Embryonic Stem Cells ◽  
Protein Kinase ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Endoderm Differentiation ◽  
Extraembryonic Endoderm ◽  
Kinase C

Phorbol esters activate protein kinase C and glucose transport and can replace the requirement for growth factor in interleukin-3-dependent multipotent stem cells

1986 ◽  
Vol 84 (1) ◽  
pp. 93-104 ◽  
Author(s):  
A.D. Whetton ◽  
C.M. Heyworth ◽  
T.M. Dexter
Keyword(s):  
Stem Cells ◽  
Protein Kinase C ◽  
Growth Factor ◽  
Protein Kinase ◽  
Dna Synthesis ◽  
Glucose Transport ◽  
Hexose Transport ◽  
Cellular Survival ◽  
Interleukin 3 ◽  
Kinase C

Interleukin 3 (IL-3) promotes the survival, proliferation and development of progenitor cells from several distinct haemopoietic lineages and can also stimulate the self-renewal of stem cells. We have explored the mode of action of this growth factor in promoting survival and proliferation, using a multipotent haemopoietic stem cell line FDC-Mix 1. In the absence of IL-3 these cells died within 16–48 h. However, this requirement for IL-3 could be replaced by 12-O-tetradecanoylphorbol-13-acetate (TPA) plus Ca2+ ionophore, which promoted not only survival but also DNA synthesis with no concomitant loss of the multipotential nature of these cells. TPA and Ca2+ ionophore, respectively, could also interact synergistically with IL-3 to promote DNA synthesis. Both IL-3 and TPA stimulated the translocation of protein kinase C (PK-C) from the cytosol to a membrane-bound form in FDC-Mix 1 cells. Previously we suggested that IL-3 can activate the primary metabolism of IL-3-dependent cells so that increased glucose transport and glycolysis lead to maintenance of ATP levels and cellular survival. To investigate whether TPA and, or, Ca2+ ionophore could also influence cellular survival via an activation of glucose uptake we assessed the effects of these agents on hexose transport. TPA +/− Ca2+ ionophore activated hexose transport to the same degree as does IL-3 but these agents cannot superstimulate FDC-Mix 1 hexose transport in cells that already exhibit an activated transport system from preincubation with IL-3. We conclude that IL-3 maintains FDC-Mix 1 cells via its ability to activate PK-C and increase cytosolic levels of Ca2+, and that an IL-3-mediated activation of PK-C may promote cellular survival via its ability to enhance hexose uptake by phosphorylating the glucose transport protein.

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